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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-mREV-erb alpha

Expression vector of mouse REV-erb alpha.

Catalog number RDB14183
Resource name pcDNA3-mREV-erb alpha
Alternative name 41
Clone info. Expression vector of mouse REV-erb alpha.
Comment Expression was confirmed by the depositor with real-time quantitative RT-PCR. NM_145434 8CDS 75-1922)
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse REV-ERB alpha cDNA
Depositor|Developer Takumi, Toru |
Other clones in our bank
External Database
mouse REV-ERB alpha
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
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          RDB14183 pcDNA3-mREV-erb alpha DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3-mREV-erb alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14183).

          Reference section:

          Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14183_A6F3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB14183_A6F3a.seq check
          >D03420B6_A6F3_1_CMV-Forward_A05_02.ab1
              1 NNNNNNNNNN NNNNNTNNNN NNNNNGGGCG GTANGCGTGT NCGGNGGGNN GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACTAGTAA CGGCCGCCAG
            181 TGTGCTGGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CGGGAATTCG ATTCATGACG
            241 ACCCTGGACT CCAATAACAA CACAGGTGGT GTTATCACCT ACATTGGCTC TAGTGGCTCC
            301 TCCCCGAGCC GGACCAGCCC GGAGTCCCTC TACAGTGACA GCTCCAATGG CAGCTTCCAG
            361 TCCCTGACTC AAGGTTGTCC CACATACTTC CCACCATCAC CTACTGGCTC CCTCACCCAG
            421 GACCCTGCCC GCTCTTTTGG CAGTGCGCCA CCCAGTCTCA GTGATGATAG CTCCCCTTCT
            481 TCTGCATCAT CGTCATCCTC TTCATCCTCC TCCTCCTTCT ATAACGGGAG CCCCCCAGGA
            541 AGTCTACAAG TGGCCATGGA AGACAGCAGC CGAGTGTCCC CCAGCAAGGG CACAAGCAAC
            601 ATTACCAAGC TGAATGGCAT GGTGCTACTG TGTAAGGTGT GTGGGGACGT GGCCTCAGGC
            661 TTCCACTATG GAGTGCACGC CTGTGANGGC TGCAAGGGCT TTTTTCGCCG GANCATCCAA
            721 CAGAATATCN AGTACAAACG GTGTCTGAAA AACGAAAACT GCTCCATCGT TCGCATCAAT
            781 CGCAACCGCT GCCAGCANTG TCGCNTCAAG AANTGTCTCT NCGTTGGCAT GTCTAGAGAT
            841 GCTGNGCGTT TNNGNCNNAT NCCCCNAGAN ANANANNNNA NCGNNNNCNN GNNGANNTGC
          901 NNNNCGCNAT NNNNNNNNNC NANNANCCAN CTNNNCNNCN NNNNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'
          Sequence file: RDB14183_A6F3b.seq check
          >D03420B6_A6F3_1_BGH_rev2_B05_05.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNG GGNNAACAAC AGNTGGCTGG CNACTAGAAG
             61 GCACAGTCGA GGCTGATCAG CGAGCTCTAG CATTTAGGTG ACACTATAGA ATAGGGCCCT
            121 CTAGATGCAT GCTCGAGCGG CCGCGAATTC ACTAGTGATT TCACTGGGCG TCCACCCGGA
            181 AGGACAGCAG CTTCTCGGAA TGCATGTTGT TCAGGGTCCG CAGGTCCGGC AGCTTGAGCA
            241 GCAGCTTGGT GAAGCGGGAA GTCTCCGAGG GCCGGTTCTT CAGCACCAGA GCCCGAAGAG
            301 CCCGCAGCAG CGTCTCCTGG AGCTGCTCCA CCGAAGCGGA ATTCTCCATT CCCGAGCGGT
            361 CTGCAGAGAC AAGCACCACC GCGGTGAATA GGCCCAGCTC CTCCTCGGTA AGTGCCAGGG
            421 AGTTGAGCTT CTCGCTGAAG TCAAACATGG CATTGAGCAG GTCGCCCATG CCCATGGCAC
            481 CGAGCTCCTG CAGACTGTAG GTTGTGCGGC TCAGGAACAT CACTGTCTGG TCCTTCACGT
            541 TGAACAACGA TGCAAAGCGC ACCATCAGCA CCTCAAAGGT GCCAGCCTTA AGCAGGGTCA
            601 CCTGGTCGTG CTGAGAAAGG TCACGGAAGC CTGGGATGTG TTTGGCAAAC TCTACCACCT
            661 CCCGAACGGC AGGCGTGAAG CTCATAGAGA AGTCTTCCCA GATCTCCTGC ACAGTCCGGC
            721 CGCTGCGTCC ATGGGGATAC ATGTTCATGG GACATGCNAG CAGAACATTC TTGGTGTTGC
            781 CTTGCCGTAN ANTGTTGGCA NGTGCCTCCC CTTCTGGGGC CGAATATACG TGGGTGGGGC
            841 ACAGACGGNG CCNATTGCTG TTANGTTGGT GGCANCTGNG GNGNGTNNNN NNGAANNNCC
          901 NNNTANGCGG GTANNANNNA NNGNCCNNNN NNTAGANNNN TNCNNNGNNN NNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB14183_A6F3c.seq check
          >D03420B6_A6F3_1_SV40pro_F_C05_08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT TTGGNNGCCT ANGCTTTTGC
             61 NNNNNGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
            121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
            181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
            241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
            301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
            361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
            421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
            481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
            541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
            601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
            661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTNCC GAATATCATG
            721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
            781 TATCAGGACA TAGCGTTGGC TACCCGNGAT ATTGCTGAAN ANCTTGNCGG CNAATGGGGC
            841 TGNNCGCTTC CTCGNGCTTT ACNGNATCGC CNCTCCNGAT NNNCANCGCN TCNNNNNNNA
            901 TCNNCNTTNN NNNCGANTTC NTNNNGANCN NGNNTCNGGG GGNTNCNAAA TGNNNNNNNN
          961 NN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl