Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-myc-GFP-TNRC6A

Expression vector of myc-GFP-tagged human TNRC6A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14214_A6Fup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14214_A6Fua.seq
>D03279A2_A6Fu_1_CMV-Forward_A07_01.ab1     1 ACGTAATAAC CATCCGCCCC ATTGAACGCA AAATGGGGCG GTAGGCGGTG TACGGTGGGA    61 AGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG   121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GCCCACCATG GAACAAAAAC   181 TCATCTCAGA AGAGGATCTG TCCGGCGGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG   241 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT   301 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA   361 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT   421 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG   481 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG   541 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT   601 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG   661 TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAAGG TGAACTTCAA AGATCCGCCA   721 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG   781 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA   841 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAA   901 TCACTTCTCG GCAATGGACG AAGCTGTACA AGGTCCCGGG CGGCAAAGCT TTAATGGGAT   961 GCCTGGATTC CTGCCTTCCC AGTTCTGGAA ATCAGAAGAA GAAACAATCG ACTATCATGG  1021 GCTTTCAAGG AAACCACAGG GTGGGTGGAA AAAAAAGATG GGCCCCTTTC GGGAAATAGC  1081 AACTGGGAAA CCTTTGGGGT TCCCCCAAAA ACCAAGCTTT GGGTAGCGTT GGGGATAACC 1141 CAGCCAGAGC CCATAATAAA TA //
Primer: TNRC6A_GW1 Sequence file: RDB14214_A6Fub.seq
>D03279A2_A6Fu_1_TNRC6A_GW1_A04_01.ab1     1 AAAAAATGCG GTGCTATGGT TCTATTACTC TGGAGACAAA TGTTCAGGCC CTAATGGCCA    61 AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT GGCGTAAATG GTCCTATGGG   121 CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG TGGGAATCTG GTGCAGCAAA   181 CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT TCTGGAGGAA GTCGAAGAGG   241 ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC AGCGTTGAGT GGAACAAACT   301 GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT GGTAAGACGT TTACAAATGG   361 ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA TCTCAGACAA ATGAGCAAAG   421 CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT GGTAGTACAG AAAGCACTGG   481 ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA GACAGAAGAA AAATTGATCA   541 GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA GATCCACGTG TCCTGTCCAA   601 CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC TGGGATACAG AAACATCACC   661 TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG GGAAGCTCTG CAACACAGAC   721 TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT GGTAATGATA CCTCATCTGT   781 ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA GATTCCAAAG GCTCAAACTG   841 CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG GTCAAGAGCA ATCAGTGGGG   901 GGAAATTGCA AGAGGAGAAA GCTGCATGGA AATGACTCGC AAAAGAATAA ACAGGGATGG   961 GGGTGATGGG ACAAAAATCA AGCCAAAGGT GGTCCTGTTT CTGCCAGTGA TACTGGGGAG 1021 AACCTTCAGG ATATCATGGG TGAAGGCCCA ATAAGAAAAT CCAGCCCTTC AAG //
Primer: TNRC6A_GW2 Sequence file: RDB14214_A6Fuc.seq
>D03279A2_A6Fu_1_TNRC6A_GW2_H04_22.ab1     1 CCACAACCAC GGCCAGGGGG GGGGTGGGAA GATGATTCTG CTGCTACAGG AATGGTCAAG    61 AGCAATCAGT GGGGGAATTG CAAAGAGGAG AAGGCTGCAT GGAATGACTC GCAAAAGAAT   121 AAACAGGGAT GGGGTGATGG ACAAAAATCA AGCCAAGGGT GGTCTGTTTC TGCCAGTGAT   181 AACTGGGGAG AAACTTCAAG GAATAACCAT TGGGGTGAGG CCAATAAGAA ATCCAGCTCA   241 GGAGGTAGTG ACAGTGACAG GTCCGTTTCC GGTTGGAACG AACTTGGTAA AACTAGTTCT   301 TTCACTTGGG GAAACAACAT AAATCCAAAT AATTCATCAG GATGGGATGA ATCTTCTAAA   361 CCTACTCCTT CCCAGGGATG GGGAGACCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA   421 GATTCGTCAA AGCCAGTCAG CTCTCCAGAC TGGAACAAGC AACAAGACAT TGTTGGATCT   481 TGGGGAATCC CACCAGCTAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG GGGACCTATA   541 CCAGCCCCAG CAAAAGAAGA AGAACCCACA GGCTGGGAGG AACCATCCCC AGAATCTATA   601 CGTCGCAAAA TGGAGATTGA TGATGGAACT TCAGCTTGGG GAGATCCAAG CAAATACAAC   661 TACAAAAATG TGAACATGTG GAACAAAAAC GTCCCAAATG GCAACAGCCG TTCAGACCAG   721 CAAAGCACAG GTACATCAGC TGCTAACGCC TGGCAAGTGC CATCTCAAAC AAAGAGGCAA   781 GCAGTGGCTC TGGCTGGGGG TGAGCCCTGG GGGGGAGCCT TTCTACTCCA GCCACAACTG   841 TGGATAATGG TACTTCAGCA TGGGGGTAAG CCCATAGACA GTGGGTCCCA GCTGGGGGGG   901 AACCCATTGC TGCGGCATCC AGCACATCCA CGTGGGGCTC CAGCTCTGTT GGGTCCACAA   961 GCATTAAGCA AATCTGGGCC AAAATCTATG CAAGATGGCT GGGTGTGGGT GGATGATATG  1021 CCAATTGCCT GGGAAATCGC CCCACTGGCT GGGAAGAGGA AAGAGGATGT GGAGATTGGA  1081 ATGTGGAATA GTTAATTCCA TCTCAAGAAG CTTGACTTCA TTCTTTAATG GCCACCATAT  1141 CCAAAGGAAA ATGTCATCGA AGGTCTTTGA ATGGCAAAAG GAAAAAGGAC ATAGGGGTTG 1201 A //
Primer: TNRC6A_NES-NLS-GW3 Sequence file: RDB14214_A6Fud.seq
>D03279A2_A6Fu_1_TNRC6A_NES-NLS-GW3_D07_10.ab1     1 TGGGCTGGCT GATATATTGA TGGGGAGCTT CATGCTTTGA CTGGACATAA ACTGCATGTT    61 TTGGGATTCA TCTGCTACAA TGCCATCCTT ATCAAAATAA GGATTGCGCT CCATGGAAGA   121 CTCTTTGGAA ATCTGAGGCC GAGAACCAGG GCCTTTCCCG ACCGTTCGAT TGTAATCACC   181 AATATTTAGG CTATGTTTAT CTATCTCCAT TCGTTTGTCT TGTAACATTC CTCCAGAAAG   241 GTCCATCTTA TTGCTTTGTA CATTTTCCTC TGGTGAGTCT CTCGAAAAAC TGATGTTTGA   301 AAACTGTTTA ACAAATGGAT TTATCCACGC TTCTTCTTGT TTGTTTCCAC CTTTCATCAT   361 TCCCCTTTCC CTTCTCCTTT TTTTGCCACT CAGACCCTTC GATGACATTT TCTTTGTATA   421 TGGTGGCCAA TTTAAAGATG AGTTAAGCTC TTGAGATGAA TTACTATTCC ACATTCCAAT   481 CTCCACATCC TCTTCCTCTT CCCAGCCAGT GGGGCGATTT CCAGGCAATG GCATATCATC   541 ACCACACCAG CCATCTTGCA TAGATTTTGG CCCAGATTTG CTTAATGCTT GTGGACCAAC   601 AGAGCTGGAG CCCCACGTGG ATGTGCTGGA TGCCGCAGCA ATGGGTTCCC CCCAGCTGGG   661 ACCACTGTCT ATGGGCTTAC CCCATGCTGA AGTACCATTA TCCACAGTTG TGGCTGGAGT   721 AGAAGGCTCC CCCCAGGGCT CACCCCAGCC AGAGCCACTG CTTGCCTCTT TGTTTGAGAT   781 GGCACTTGCA GGCGTTAGCA GCTGATGTAC CTGTGCTTGC TGGTCTGAAC GGCTGTTGCC   841 ATTTGGGACG TTTTTTGTTC CACATGTTCA CATTTTTTGT AGTTGTATTT GCTTTGGATC   901 TCCCCAAGCT GAAGTTCCAT CATCAATCTT CCATTTTTGC GACGTATAGA TTCTGGGGGA   961 TGGTTCCTTC CCAGCCCTGT GGGTTTCTTC TTTCTTTTTG CTGGGGGCTG GGTATAAGGT  1021 TCCCCCCCAG CCCAGCCTGT ACCCAAGGAG GTTTTGGCCC TGTAGCCTTG ATGGGGATTT 1081 CGTCAAGAAT CCCACATTGT CTGATGCCTT GATTCAGTTC TTTGGAGA //
Primer: EGFP-C Sequence file: RDB14214_A6Fue.seq
>D03279A2_A6Fu_1_EGFP-C_B01_04.ab1     1 CAGGCATCTC GGGCATGGAC GAGCTGTACA GTCCGGCGGC AAGCTTATGG ATGCTGATTC    61 TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG GCTTCAGGGA ACACAGGTGG   121 TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC CAAAACAAGT TTGTAGTTGG   181 TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG CCATGGGGTT TTTCCCATGG   241 AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT GAAAGCAAAT CTGAAAGTAG   301 CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA TCAAATGGAG GGTTAAATCC   361 AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA GTATTAGAGA ACAATGGACT   421 TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT AATATTCAGT GCAGTACTAT   481 AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG AGTGGTGGTT CTACCCATGG   541 TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA AGTGGTACAC AGAAGGTTTC   601 ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT GGACCAAATA ACACTACTAA   661 CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG AATAATGAGC TGCCTAGTAG   721 TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT CAGATGCAGC TCCATCAGTA   781 TGAATGGCAC TTCCCTTTCT CACCTTAGCA ATGGGAGAGT CAAAAAGTGG GAGGCTCTTA   841 TGGTACTACA TGGGGTGCCC TATGGGTTCT AATTTACTCT GGGAGACAAA TGTTCAGCCC   901 TAAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT ATGCAGCTGT GTAAATGGTC   961 CTATGGGCAC TACCTTTCCA GGTAACACAA ACAAAGGAGT GGTGTGTGGA TCTGTCCAGC 1021 ACTTCCAGAG TAACTCATGG ATGATGCGCA ATCTGA //
Primer: BGH_rev2 Sequence file: RDB14214_A6Fuf.seq
>D03279A2_A6Fu_1_BGH_rev2_C04_07.ab1     1 CTATCGGATA GGAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CATGGACTCT CCACCCCCAC   121 CCAGGTGGTC AACAGAAAGA AAAGCGTTAA TGGGAGATGG GCTGCTAATT CCTCGGGGGT   181 CGCTGCTGCT TGGGGGACCC CACAGGCTTG TGGAATAATG CGGGGTCCCC CAGAGTGAAG   241 TGCCGTGAAG GTCTCCACAG TTAGTTCCCG ACAGCCCAGC ACCATTCCAG TGATTGAGAT   301 CGGTCCGGCT GGAGAATGAG TGGGAACAGT CGAGGGAGCC CAGCCGGCTC TGGCTGGACC   361 CGAGAGACTG CCAGCCGGGA GAAGGGGTCA GAGACTGGCT TTGTGCAAAG AAACGACTGA   421 TCTCCTCTTC ACTGGCAAAC TCAGCAAGAA TAGTAGTGTT CCCCAGTACA CACATGTGCA   481 GAGACTTTTG TGCCTTCACT ACCTCTTCTT TTGAACTGTA GCGGACCAGA GCATTTCCGT   541 GAGGGAGGTT CAGGTGGAAT GTGATCAGCG GGCCGTGCTG CATGCACAGA GTGCGCAGAG   601 TTGAGCCATC GATCTGAGGT GTAAGGTTTT TTAGAACAAG CCAATTTGTT ATTCTCCCTG   661 AGCTGCTCTC ACCCCAGCTG GAACCTGGGG TATATCTGGC GTCAGAATTT CCCCATCCTC   721 CACCTATACG AAGGGGAGAA TTATCCCACG TAGACAAGGG TGGCTTCTGA CCAGTCAGTC   781 CCGGAGGTGG GCGGGACGGA GCAGTGATGT TTTTTAGGTG GCAAAGGGAC CTTCCACAGC   841 TCATGAGCCA GAGAGGTGTT TGTAACTGAA CCAGGAGACC ATGTCAATTT GGAATCACTA   901 TTTCTGGCTG AAGTGCTTTG TGCTGTACTG CTGAGGGGAA CGTTGTAGTT TGGAGCACGA   961 ATGGATGACC AGGGCACTAG TTGAAGCAGC GTATGTTTCA CGATGAAGAA TGACCCACTG  1021 TTTCTGGTCC ATGAAGTTGA TTCAAACCTT GCCGCACAGT ATAAGTGCCA AAGGACGGAT 1081 TTTAGTGAAC CACTG //
Primer: SV40pro_F Sequence file: RDB14214_A6Fug.seq
>D03279A2_A6Fu_1_SV40pro_F_D04_10.ab1     1 CCTGGTCTGG GCTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT   121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC   661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC   781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC   841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC   901 CTTCTTGACG AGTTCTTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCC GACCAAGCGA   961 CGCCCAACCC TGCCAATCAC GAGATTTCGA TTTCACCCGC CCGGCGTTCT ATGAAAGGAT  1021 GGGCTTCGGA TCGATTTTCA GGACGTCGGC TGGATGAATC CATCCAGTCG GCGGGGGGAA 1081 TCGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl