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pSUPER-retro-puro-DsRed

Retroviral packaging vector for shRNA of DsRed fragment.

Catalog number RDB14240
Resource name pSUPER-retro-puro-DsRed
Clone info. Retroviral Packaging Vector for shRNA of DsRed fragment.
Comment Expression was confirmed by the depositor with RNAi assay.
Vector backbone pSUPER.retro.puro (Plasmid)
Size of vector backbone 7.3 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cell).
Gene/insert name Discosoma sp red fluorescent protein (RFP) cDNA
Depositor|Developer Ui-Tei, Kumiko |
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External Database
Discosoma sp red fluorescent protein (RFP)

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., Nucleic Acids Res. 43 (20): 9856-9873, 2015). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., Nucleic Acids Res. 43(20):9856-9873, 2015)。教育研究目的での使用に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB14240 pSUPER-retro-puro-DsRed Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

            2022.05.17

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