Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFPC1-mouse Rab8A-Q67L - constitutive active

Expression vector of EGFP-tagged mouse Rab8A constitutive active (CA) mutant.

Catalog number RDB14275
Resource name pEGFPC1-mouse Rab8A-Q67L - constitutive active
Clone info. Expression vector of EGFP-tagged mouse Rab8A constitutive active (CA) mutant; Q67L. CA mutant mimics the GTP-bound form.
Comment Reference sequence is S53270. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab8A cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab8A

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14275 pEGFPC1-mouse Rab8A-Q67L - constitutive active DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14275_A6H4p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP,insert 5'(Q67L)
          Sequence file: RDB14275_A6H4a.seq check
          >D03692A5_A6H4_2_EGFP-C2_G08_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNTCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT
             61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGCGAAGAC CTACGATTAC
            121 CTGTTCAAGC TGCTGCTGAT CGGGGACTCG GGGGTAGGGA AGACCTGTGT CCTGTTCCGC
            181 TTCTCCGAGG ACGCCTTCAA CTCCACATTC ATCTCTACCA TAGGAATTGA CTTTAAAATT
            241 AGGACCATAG AGCTCGATGG CAAGAGGATT AAACTGCAGA TATGGGACAC AGCCGGCCTG
            301 GAGCGGTTTC GAACAATCAC GACAGCCTAC TACAGGGGTG CCATGGGTAT CATGCTGGTC
            361 TACGACATTA CCAATGAGAA GTCCTTTGAC AACATCCGGA ATTGGATTCG GAACATTGAA
            421 GAGCATGCCT CTGCAGACGT GGAGAAGATG ATACTGGGGA ATAAGTGTGA TGTGAATGAC
            481 AAGAGACAGG TGTCCAAGGA ACGGGGAGAA AAGCTGGCAC TCGACTATGG GATCAAGTTC
            541 ATGGAGACCA GTGCAAAGGC CAACATCAAT GTGGAGAATG CATTTTTCAC TCTTGCCAGG
            601 GATATCAAAG CAAAAATGGA CAAAAAATTG GAAGGGAACA GCCCGCAGGG GAGCAGCCAT
            661 GGAGTCAAGA TCACAGTGGA GCAGCAGAAG ANGANCAGCT TCTTCCGGTG CAGTCTCCTG
            721 TGAAATCACT AGTGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGNTCTA
            781 GATAACTGAT CATAATCAGC CNTACCACAT TTGTAGAGGT TTTACTTGCT NTAAAAAACC
            841 TCCCACACCT NCCCCTGAAC CNGAAACATA AAATGAATGN NNTTGNTGNT GTTAACTTGT
            901 TNANTGNAGC TTATNATGGT TANNAATAAA GCNANAGCAT CNNAAATTTC NCAANNNAAG
          961 NATTTTTTTT CNNNGNNNTN NNNNNGNNGN NTNNNCNNAN NNNNNNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'(Q67L)
          Sequence file: RDB14275_A6H4b.seq check
          >D03692A5_A6H4_2_SV40polyA-R2_H08_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNAACN AGTTANCNAN NACNATTGCA TTCNTTTTAT GTTTCNGGTT
             61 CNGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
            181 ATTCACTAGT GATTTCACAG GAGACTGCAC CGGAAGAAGC TGGTCCTCTT CTGCTGCTCC
            241 ACTGTGATCT TGACTCCATG GCTGCTCCCC TGCGGGCTGT TCCCTTCCAA TTTTTTGTCC
            301 ATTTTTGCTT TGATATCCCT GGCAAGAGTG AAAAATGCAT TCTCCACATT GATGTTGGCC
            361 TTTGCACTGG TCTCCATGAA CTTGATCCCA TAGTCGAGTG CCAGCTTTTC TCCCCGTTCC
            421 TTGGACACCT GTCTCTTGTC ATTCACATCA CACTTATTCC CCAGTATCAT CTTCTCCACG
            481 TCTGCAGAGG CATGCTCTTC AATGTTCCGA ATCCAATTCC GGATGTTGTC AAAGGACTTC
            541 TCATTGGTAA TGTCGTAGAC CAGCATGATA CCCATGGCAC CCCTGTAGTA GGCTGTCGTG
            601 ATTGTTCGAA ACCGCTCCAG GCCGGCTGTG TCCCATATCT GCAGTTTAAT CCTCTTGCCA
            661 TCGAGCTCTA TGGTCCTAAT TTTAAAGTCA ATTCCTATGG TAGAGATGAA TGTGGAGTTG
            721 AAGGNGTCCT CGGANAANCG GAACAGGACA CAGGTCTTCN CTACCCCCGA GTCNNNGATC
            781 AGCAGCAGCT TGAACAGGTA ATCGTANGTC TTCGNCNTNG NTCTGANTCC GGANTTGTAC
            841 AGCTCGTCCA TGCCGANAGT GATNCCGGCG GCGGNCACNA ACTNCANCAN GANCNTGTGA
            901 TCGCNCTTCT CNNTGGGGNC TTTNCTCNNG NNNNNNTGGG NGCNCNNNNA NNNGNNNGNC
            961 NGNNNNCANC NNNGGNNCNN NNNNCNNNGG GGGNNNNCTG NTNNNNNNNG NNNNGNNANN
          1021 NNNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: .CMV Pro,EGFP
          Sequence file: RDB14275_A6H4c.seq check
          >D03692A5_A6H4_2_CMV-Forward_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNTGNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
            121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
            181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
            241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
            301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
            361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
            421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
            481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
            541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
            601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
            661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
            721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
            781 TTCGTGACCG CCGCCGGGNT CACTCTCGGC ATGGNCGANC TGTACAAGTC CNNANTCANA
            841 TCCATGGNGA ANANCTACGA TTACCNNNTC AAGCTGCTGC TNNTCGGGGA NTCNGGGNNA
          901 NGNAANANCN NNGNCCNNNT NCGNNTCTNN NNNNANNNNN NNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB14275_A6H4d.seq check
          >D03692A5_A6H4_2_SV40pro_F_V2_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNNG CTTTTGCAAG NNCGATCNAG AGNNAGGNNG AGGNNCGTTT
             61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
            601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN
            721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
            781 TTCCTCNTGC TTTACGGTAT CGCCGCTCCN GATTCGCANC GCATCGCCTT CTATCGCCTT
            841 CTTGACGANN NCTTCTGANC GGGANTCTGG GGTTCGAAAT GNNNNANCNA NCGACGCCCA
            901 ACCNNNCNTC NCNANANTTC NNNTNCNNCN NCNNCNTTCN ANNAANNNNN NGGNCNNNNN
          961 NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl