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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFPC1-mouse Rab10-T23N - constitutive negative

Expression vector of EGFP-tagged mouse Rab10 constitutive negative (CN) mutant.

Catalog number RDB14278
Resource name pEGFPC1-mouse Rab10-T23N - constitutive negative
Clone info. Expression vector of EGFP-tagged mouse Rab10 constitutive negative (CN) mutant; T23N. CN mutant mimics the GDP-bound form.
Comment Reference sequence is NM_016676. Expression was confirmed by the depositor with fluorescent microscopy.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab10

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al., J. Cell Sci. 125, 5177-5187, 2012); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
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          RDB14278 pEGFPC1-mouse Rab10-T23N - constitutive negative DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDb14278_A6H4p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDb14278_A6H4a.seq check
          1
          Primer: ME-1290RV
          Sequence file: RDb14278_A6H4b.seq check
          1
          Primer: SV40pro_F_V2
          Sequence file: RDb14278_A6H4c.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          2023.04.10

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