Resource data sheet
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pcDNA3.1_V5-NECAB3_WT (#RDB14306)

Expression vector of V5-tagged human NECAB3 gene; wild type.

Clone info. Expression vector of V5-tagged human NECAB3 gene; wild type, see Fig. 5A for details.
Comment Expression was confirmed by the depositor with immunoprecipitation and real-time PCR.
Vector backbone pcDNA3 derivative (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human NECAB3 cDNA
Depositor|Developer Sakamoto, Takeharu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, H.J. et al., Sci. Rep. 6: 22784, 2016)
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakaoka, H.J. et al., Sci. Rep. 6: 22784, 2016)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14306 pcDNA3.1_V5-NECAB3_WT Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1_V5-NECAB3_WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14306).

Reference section:

Nakaoka, H.J., Hara, T., Yoshino, S., Kanamori, A., Matsui, Y., Shimamura, T., Sato, H., Murakami, Y., Seiki, M., Sakamoto, T., NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells. Sci. Rep, 6: 22784 (2016).
PMID 26948053. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakaoka, H.J., NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells. Sci. Rep, 6: 22784 (2016).
PMID 26948053. [link to RRC of NBRP]