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pBabe-hSTING(C88/91S)

Retroviral expression vector of human STING, C88/91S mutant.

Catalog number RDB14352
Resource name pBabe-hSTING(C88/91S)
Clone info. Mammalian retroviral vector for expressing human STING (dual mutation, C88S/C91S). Cysteine residues are the site of protein palmitoylation. The double mutation of Cysteine results in loss of the palmitoylation.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
Other clones in our bank

External Database
human TMEM173

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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          RDB14352 pBabe-hSTING(C88/91S) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14352_A6J7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14352_A6J7a.seq check
          >D03866A2_A6J7_1_pBABE5'_B01_04_ABI24.ab1
              1 GTGTGTTCTG GAACAAGTGT GGTGGTACGT AGGAATTCGC CGCCACCATG CCCCACTCCA
             61 GCCTGCATCC ATCCATCCCG TGTCCCAGGG GTCACGGGGC CCAGAAGGCA GCCTTGGTTC
            121 TGCTGAGTGC CTGCCTGGTG ACCCTTTGGG GGCTAGGAGA GCCACCAGAG CACACTCTCC
            181 GGTACCTGGT GCTCCACCTA GCCTCCCTGC AGCTGGGACT GCTGTTAAAC GGGGTCTGCA
            241 GCCTGGCTGA GGAGCTGCGC CACATCCACT CCAGGTACCG GGGCAGCTAC TGGAGGACTG
            301 TGCGGGCCAG CCTGGGCAGC CCCCTCCGCC GTGGGGCCCT GTTGCTGCTG TCCATCTATT
            361 TCTACTACTC CCTCCCAAAT GCGGTCGGCC CGCCCTTCAC TTGGATGCTT GCCCTCCTGG
            421 GCCTCTCGCA GGCACTGAAC ATCCTCCTGG GCCTCAAGGG CCTGGCCCCA GCTGAGATCT
            481 CTGCAGTGTG TGAAAAAGGG AATTTCAACG TGGCCCATGG GCTGGCATGG TCATATTACA
            541 TCGGATATCT GCGGCTGATC CTGCCAGAGC TCCAGGCCCG GATTCGAACT TACAATCAGC
            601 ATTACAACAA CCTGCTACGG GGTGCAGTGA GCCAGCGGCT GTATATTCTC CTCCCATTGG
            661 ACTGTGGGGT GCCTGATAAC CTGAGTATGG CTGACCCCAA CATTCGCTTC CTGGATAAAC
            721 TGCCCCAGCA GACCGGTGAC CATGCTGGCA TCAAGGATCG GGTTTACAGC AACAGCATCT
            781 ATGAGCTTCT GGAGAACGGG CAGCGGGCGG GCACCTGTGT CCTGGAGTAC GCCACCCCCT
            841 TGCAGACTTT GTTTGCCATG TCACAAATAC AGTCAAAGCT GGCTTTAGCC GGGAGGATAG
            901 GCTTGAGCAG GCCAAAACTC TTTCTGCCGG ACACTTGAGG ACATCCCTGG CAGATGCCCC
            961 TGAGTCTCAG AACAACTGCC GGCCTTCATT TGCCTACCAG GAACCCTGCA GAATGACAGC
           1021 AGCTTTCTCG CTGGTCCCAA GAGATTCTTC CGGCACCCTG CGGCAGGAGG AAAATGAGAG
           1081 GGTTAACTGT GGGGCCAGCC TGGAGACTTC AACGTTGCCC AAGTATACCC TCCCACGGAA
          1141 TGGATTCCCA
          //
          Primer: PuroR-rev
          Sequence file: RDB14352_A6J7b.seq check
          >D03866A2_A6J7_1_PuroR-rev_B06_02_ABI08.ab1
              1 GCTTAGACTT TTTGCAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC
             61 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC
            121 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT
            181 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG
            241 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT
            301 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGGGT CGAGTCAAGA
            361 GAAATCCGTG CGGAGAGGGA GGGGCTTTTC CATTCCACTG ATGAGGAGCT CAGGCTCTTG
            421 GGACATCGTG GAGGTACTGG GCACCGCTGA GGTCTTCAAG CTGCCCACAG TAACCTCTTC
            481 CTTTTCCTCC TGCCGCAGGT GCCGGAGAAC CTCCTGGGAC AGCGAGAAGC TGCTGTCATC
            541 TGCAGGTTCC TGGTAGGCAA TGAGGCGGCA GTTGTTCTGA GACTCAGGGG CATCTGCCAG
            601 GATGTCCTCA AGTGTCCGGC AGAAGAGTTT GGCCTGCTCA AGCCTATCCT CCCGGCTAAA
            661 GCCAGCTTGA CTGTATTGTG ACATGGCAAA CAAAGTCTGC AAGGGGGTGG CGTACTCCAG
            721 GACACAGGTG CCCGCCCGCT GCCCGTTCTC CAGAAGCTCA TAGATGCTGT TGCTGTAAAC
            781 CCGATCCTTG ATGCCAGCAT GGTCACCGGT CTGCTGGGGC AGTTTATCCA GGAAGCGAAT
            841 GTTGGGGTCA GCCATACTCA GGTTATCAGG CACCCCACAG TCCAATGGGA GGAGAATATA
            901 CAGCCGCTGG CTCACTGCAC CCCGTAGCAG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
            961 CCGGGCCTGG AGCTCTGGCA GGATCAGCCC GCAGATATCC GATGTAATAT GACCATGCCC
           1021 AGCCCATGGG CCACGTTGAA ATTCCCTTTT TTCACAACAC TGCAGAGATC TCAGCTGGGG
           1081 GCCAGGCCTT GGAGCCAGAG ATGTCAGTGA CTTGCCAGAG CCAGAGGGCA AGCATCCAAG
          1141 TGACGGCGCG ACGCAATTGG CAGGGAGCTT AGAT
          //
          Primer: Amp_R
          Sequence file: RDB14352_A6J7c.seq check
          >D03866A2_A6J7_1_Amp_R_B03_06_ABI24.ab1
              1 CTCCCTTTTT ACATATTTAT TGAAGGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
             61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
            121 GTGCCACCTG CAGCCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC
            181 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT
            241 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC
            301 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC
            361 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC
            421 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA
            481 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG
            541 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT
            601 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC
            661 CGGGAGGCAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG ATTGTCTAGT GTCTATGACT
            721 GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT
            781 GGTGGAACTG ACGAGTTCTG AACACCCGGC CGCAACCCTG GGAGACGTCC CAGGGACTTT
            841 GGGGGGCCGT TTTTGTGGCC CGACCTGAGG AAGGGGAGTC GATGTGGGAA TCCGACCCCG
            901 TCAGGATATG TGGTTCTGGG TAGGAGACGA GAAACCTAAA ACAGGTTCCC GCCTCCGTCT
            961 GAATTTTTGC TTTCGGTTTG GAACCGAAAG CCGCGCGTCT TGTCTGCTGC AGCGCTGCAG
           1021 CATCGTTTCT GTGTTGGTCT CTGTTCTGGA CTGGTGTTTC TGGTATTTGT CTGAAAATTT
           1081 AGGCAGACTT GTTTACCACT TCCTTAGGTT GACCTAAGGT CACTGGAAAG AAATGGTTCG
          1141 TAAGCCGG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14352_A6J7d.seq check
          >D03866A2_A6J7_1_SV40pro_F_V2_B05_05_ABI24.ab1
              1 GAGCGGCTTT TTTGGAGCTA GGCTTTTGCA AAAGCTTACC ATGACCGAGT ACAAGCCCAC
             61 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT
            121 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC
            181 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
            241 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
            301 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
            361 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
            421 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
            481 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
            541 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
            601 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA
            661 GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG
            721 TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT
            781 AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG
            841 GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
            901 CTGCCCCGGC TCAGGGCCAA GAACAGATGG AAACAGCTGA AATATGGGCC CAAACAGGAT
            961 ATCTGTGGGT AAAGCAGTTT CCTGCCCCCG GGCTCAGGCC CAAGAACAGA TGGGTCCCCC
           1021 AGATGCGGGT CCAAGCCCTC CAGCAGTTTT CTAGAGAATC ATCAGATGGT TTCCAGGGTG
          1081 CCTCAGGAAC TTGAATGAAC CTTGTTGCCG TTAATTTTGA ACCTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl