Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBabe-hSTING(N154S)

Retroviral expression vector of human STING, N154S mutant.

Catalog number RDB14354
Resource name pBabe-hSTING(N154S)
Clone info. Mammalian retroviral vector for expressing human STING (mutation, N154S). The disease (SAVI) patients have a point mutation of STING (V147L, N154S or V155M). These STING variants exhibit a gain-of-function phenotype and are able to stimulate the production of an IFN beta reporter construct.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
Other clones in our bank

External Database
human TMEM173

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14354 pBabe-hSTING(N154S) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14354_A6J7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14354_A6J7a.seq check
          >D03866A4_A6J7_1_pBABE5'_D07_04_ABI08.ab1
              1 GGGGTCGGAT CAGTGTGGTG GTACGTAGGA ATTCGCCGCC ACCATGCCCC ACTCCAGCCT
             61 GCATCCATCC ATCCCGTGTC CCAGGGGTCA CGGGGCCCAG AAGGCAGCCT TGGTTCTGCT
            121 GAGTGCCTGC CTGGTGACCC TTTGGGGGCT AGGAGAGCCA CCAGAGCACA CTCTCCGGTA
            181 CCTGGTGCTC CACCTAGCCT CCCTGCAGCT GGGACTGCTG TTAAACGGGG TCTGCAGCCT
            241 GGCTGAGGAG CTGCGCCACA TCCACTCCAG GTACCGGGGC AGCTACTGGA GGACTGTGCG
            301 GGCCTGCCTG GGCTGCCCCC TCCGCCGTGG GGCCCTGTTG CTGCTGTCCA TCTATTTCTA
            361 CTACTCCCTC CCAAATGCGG TCGGCCCGCC CTTCACTTGG ATGCTTGCCC TCCTGGGCCT
            421 CTCGCAGGCA CTGAACATCC TCCTGGGCCT CAAGGGCCTG GCCCCAGCTG AGATCTCTGC
            481 AGTGTGTGAA AAAGGGAATT TCAGCGTGGC CCATGGGCTG GCATGGTCAT ATTACATCGG
            541 ATATCTGCGG CTGATCCTGC CAGAGCTCCA GGCCCGGATT CGAACTTACA ATCAGCATTA
            601 CAACAACCTG CTACGGGGTG CAGTGAGCCA GCGGCTGTAT ATTCTCCTCC CATTGGACTG
            661 TGGGGTGCCT GATAACCTGA GTATGGCTGA CCCCAACATT CGCTTCCTGG ATAAACTGCC
            721 CCAGCAGACC GGTGACCATG CTGGCATCAA GGATCGGGTT TACAGCAACA GCATCTATGA
            781 GCTTCTGGAG AACGGGCAGC GGGCGGGCAC CTGTGTCCTG GAGTACGCCA CCCCCTTGCA
            841 GACTTTGTTT GCCATGTCAC AATACAGTCA AGCTGGCTTT AGCCGGGAGG ATAGGCTTGA
            901 GCAGGCCAAA CTCTTCTGCC GGACACTTGA GGACATCCTG GCAGATGCCC CTGAGTCTCA
            961 GAACAACTGC CGCCTCATTG CCCTACCAGG AACCTGCAGA TGACAGCAGC TTCTCGCTGT
           1021 CCAGGAGTCT CCGCACTTGC GCAGAGAAAA GGAGAGTACT GTGGGCAGCT GGAGACTCAG
          1081 CGGTGCAGTA CCTCACGAAT GTCCAGAGCC ATGAGATCCC T
          //
          Primer: PuroR-rev
          Sequence file: RDB14354_A6J7b.seq check
          >D03866A4_A6J7_1_PuroR-rev_D06_04_ABI08.ab1
              1 GCATTGAGCT TTTGCAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG
             61 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG
            121 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC
            181 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG
            241 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC
            301 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGGG TCGAGTCAAG
            361 AGAAATCCGT GCGGAGAGGG AGGGGCTTTT CCATTCCACT GATGAGGAGC TCAGGCTCTT
            421 GGGACATCGT GGAGGTACTG GGCACCGCTG AGGTCTTCAA GCTGCCCACA GTAACCTCTT
            481 CCTTTTCCTC CTGCCGCAGG TGCCGGAGAA CCTCCTGGGA CAGCGAGAAG CTGCTGTCAT
            541 CTGCAGGTTC CTGGTAGGCA ATGAGGCGGC AGTTGTTCTG AGACTCAGGG GCATCTGCCA
            601 GGATGTCCTC AAGTGTCCGG CAGAAGAGTT TGGCCTGCTC AAGCCTATCC TCCCGGCTAA
            661 AGCCAGCTTG ACTGTATTGT GACATGGCAA ACAAAGTCTG CAAGGGGGTG GCGTACTCCA
            721 GGACACAGGT GCCCGCCCGC TGCCCGTTCT CCAGAAGCTC ATAGATGCTG TTGCTGTAAA
            781 CCCGATCCTT GATGCCAGCA TGGTCACCGG TCTGCTGGGG CAGTTTATCC AGGAAGCGAA
            841 TGTTGGGGTC AGCCATACTC AGGTTATCAG GCACCCCACA GTCCAATGGG AGGAGAATAT
            901 ACAGCCGCTG GCTCACTGCA CCCCGTAGCA GTTGTTGTAA TGCTGATTGT AAGTTCGAAT
            961 CCCGGGCCTG GAGCTCTGGC AGGATCAGCC GCAGATATCC GATGTAATAT GACCATGCCA
           1021 GCCCATGGGC CACGCTGAAT TCCCTTTTTC ACACACTGCA GAGATCTCAG CTGGGGCAGC
           1081 CTTGAGGCCA GAGATGTTCA GTGCCTGCGA GAGCAGGAGC CAGCCATCAA GTGAAGGCGG
          1141 GCGACCGCCA ATTTGGGGAA GA
          //
          Primer: Amp_R
          Sequence file: RDB14354_A6J7c.seq check
          >D03866A4_A6J7_1_Amp_R_D03_12_ABI24.ab1
              1 ATCTGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGCAGCC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
            181 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
            241 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
            301 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
            361 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
            421 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG
            481 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
            541 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
            601 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
            661 GGCAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
            721 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
            781 AACTGACGAG TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG
            841 CCGTTTTTGT GGCCCGACCT GAGGAAAGGG AGTCGATGTG GAATCCGACC CCGTCAGGAT
            901 ATGTGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCTCCGTCT GAATTTTTGC
            961 TTTCGGTTTG GAAACCGAAG CCGCGCGTCT TGTCTGCTGC AGCGCTGCAG CATCGTTCTG
           1021 TGTGTCTCTG TCTGACTGTG TTTTCTGTAT TTTGTCTGAA AAATTAGGAC AGACCTGATA
          1081 CCACTCCTTA GTTGACTTAA GTCACTGGAA AAGAATGTTC GAGCCGGGAA TCGGCT
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14354_A6J7d.seq check
          >D03866A4_A6J7_1_SV40pro_F_V2_D05_11_ABI24.ab1
              1 GGCAGCTTTT TTGGAGCTAG GCTTTTGCAA AAGCTTACCA TGACCGAGTA CAAGCCCACG
             61 GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC
            121 GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC
            181 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG
            241 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG
            301 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA
            361 CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC
            421 GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA
            481 GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC
            541 CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA
            601 CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG
            661 CGCCCGACCG AAAGGAGCGC ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT
            721 CTCCAGAAAA AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA
            781 ACGCCATTTT GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG
            841 TCAGGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC
            901 TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGGCCAA ACAGGATATC
            961 TGTGGGTAAG CAGTTCCCTG CCCCGGGCTC AGGCCCAAGA CAGATGGGTC CCCAGATGCG
           1021 GGTCCCAGCC CTCAGCAGTT CTAGAGGACC ATTCAGATGT TTCAGGTGCC CCAACGACTG
          1081 AAATGACCTG TGCTTATTTG ACTAACCAAT TCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl