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pGEX-6P-3/GST-TAT-ODD/3-0-Casp3

Bacterial expression vector of protein prodrug TOP3 that is specifically processed and activated in hypoxia-inducible factor-active cells.

Catalog number RDB14403
Resource name pGEX-6P-3/GST-TAT-ODD/3-0-Casp3
Alternative name GST-TOP3
Clone info. Bacterial expression vector of GST tagged TAT-ODD-procaspase-3 (TOP3), a fusion protein prodrug containing TAT-PTD, ODD, and procaspase-3 specifically processed and activated in hypoxia‐inducible factor‐active cells. The recombinant protein showed prospective effects in hypoxic cells in vivo as well as in vitro.
Comment Expression was confirmed by the depositor. cDNA fragment 2,046 to 2,216 of NM_001530.3 (HIF1A ODD/3-0) and 264 to 1,097 of NM_004346.3 (CASP3) were cloned. Known differences: T to C substitution at 876 of NM_004346.3 (S205P); T to C substitutuion at 1,061 of NM_004346.3 (V266, synonymous).
Vector backbone pGEX-6P-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human HIF1A cDNA human CASP3 cDNA
Depositor|Developer Kizaka-Kondoh, Shinae |
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External Database
human HIF1A human CASP3

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016) RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016)。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。

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        RDB14403 pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content HIF-1 signaling pathway (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14403_A6Kpp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: M13R
        Sequence file: RDB14403_A6Kpa.seq check
        >14403_14403_A6Kp_1_M13R_H03_08_ABI08.ab1
            1 GACTGTGGGC GGTCGGTTTT AAACGTCGTG ACTGGGAAAC CCTGGCGTTA CCCAACTTAA
           61 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
          121 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC
          181 ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT
          241 CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA
          301 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT
          361 CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG
          421 CGTTGGAATT AGCTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC
          481 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
          541 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
          601 TGAAATGAGC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT
          661 AACAATTTCA CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG
          721 GGCCTTGTGC AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT
          781 TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG
          841 TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC
          901 ATACGTTATA TAGCTGACAG GCACAACATG TTGGGTGGTT TGTCCAAAGA GCGTGCAGAG
          961 ATTTCATGCT TGAAGGGAGC GGTTTGGGAT ATAGATACGT GTTTCGAGGA GTGCATATAG
         1021 TTAAGGACTT GCGCTCTCCA AAGTTGAATT TTCTAGCAAG CCTTACTTGG AATTGCCTGA
        1081 ATTGTCGAGA TCGTTATGGT CATAAACATA GTTATAAATG GGTTGAT
        //
        Primer: pGEX-F2
        Sequence file: RDB14403_A6Kpb.seq check
        >RDB14403_14403_A6Kp_1_pGEX-F2_A12_03_ABI24.ab1
            1 TCCTATTTGG TTTGGTTTTT AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTAACT
           61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
          121 GCGACCATCC TCCAAAATCG GATCTGGAAG TTCTGTTCCA GGGGCCCCTG GGATCCCCGA
          181 ATTCCCAGCT TGACATGGCG CCTAAGAAGA AGAGGAAGAG ATCATATGGT CGTAAGAAAC
          241 GTCGCCAACG TCGCCGAAGA TCTAACCCAT TTTCTACTCA GGACACAGAT TTAGACTTGG
          301 AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC TTCGATCAGT
          361 TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA AGCACAGTTA
          421 CAGTATTCCA GCAGGTACCA ATGGAGAACA CTGAAAACTC AGTGGATTCA AAATCCATTA
          481 AAAATTTGGA ACCAAAGATC ATACATGGAA GCGAATCAAT GGACTCTGGA ATATCCCTGG
          541 ACAACAGTTA TAAAATGGAT TATCCTGAGA TGGGTTTATG TATAATAATT AATAATAAGA
          601 ATTTTCATAA AAGCACTGGA ATGACATCTC GGTCTGGTAC AGATGTCGAT GCAGCAAACC
          661 TCAGGGAAAC ATTCAGAAAC TTGAAATATG AAGTCAGGAA TAAAAATGAT CTTACACGTG
          721 AAGAAATTGT GGAATTGATG CGTGATGTTT CTAAAGAAGA TCACAGCAAA AAGGAGCAGT
          781 TTTGTTTGTG TGCTTCTGAG CCATGGTGAA GAAGGAATAA TTTTTTGGAA CAAATGGACC
          841 TGTTGACCTG AAAAAAATAA CAAACTTTTT CAGAGGGGAT CGTTGTAGAA GTCTAACTGG
          901 AAAACCCAAA CTTTTCATTA TTCAGGCCTG CCGTGGTACA GAACTGGACT GTGGCATTGA
          961 GACAGACAGT GGTTGTTGAT GATGACATGG CGTGTCAATA AAAATACCAG GTGGAGGCCC
         1021 GACCTTCTTG TTATGCATAC TTCCCACCAG CACCTGGGTT ATTAATCCCT TTGGCGGAAA
         1081 TTCCAAAGGG ATTGGCTCCT GTCATCCCAG TTCGCTTGTG CCATGCTGAA CAGTATGCCG
        1141 AACCAAGCCT TTTAAAATTT TTAATGTCAA CA
        //
        Primer: pGEX-R
        Sequence file: RDB14403_A6Kpc.seq check
        >14403_14403_A6Kp_1_pGEX-R_A03_03_ABI24.ab1
            1 TTCCATTTAC GGGAAAAGGC GCGAAGGCAG ATCGTCAGTC AGTCACGGAT GCGGCCGCTC
           61 GAGTCGACTT AGTGATAAAA ATAGAGTTCT TTTGTGAGCA TGGAGACAAT ACATGGAATC
          121 TGTTTCTTTG CATGAAAAGT AGCGTCAAAG GAAAAGGACT CAAATTCTGT TGCCACCTTT
          181 CGGTTAACCC GGGTAAGAAT GTGCATAAAT TCAAGCTTGT CGGCATACTG TTTCAGCATG
          241 GCACAAAGCG ACTGGATGAA CCAGGAGCCA TCCTTTGAAT TTCGCCAAGG ATAATAACCA
          301 GGTGCTGTGG AGTATGCATA CAAGAAGTCG GCCTCCACTG GTATTTTATG ACACGCCATG
          361 TCATCATCAA CACCACTGTC TGTCTCAATG CCACAGTCCA GTTCTGTACC ACGGCAGGCC
          421 TGAATAATGA AAAGTTTGGG TTTTCCAGTT AGACTTCTAC AACGATCCCC TCTGAAAAAG
          481 TTTGTTATTT TTTTCAGGTC AACAGGTCCA TTTGTTCCAA AAATTATTCC TTCTTCACCA
          541 TGGCTCAGAA GCACACAAAC AAAACTGCTC CTTTTGCTGT GATCTTCTTT AGAAACATCA
          601 CGCATCAATT CCACAATTTC TTCACGTGTA AGATCATTTT TATTCCTGAC TTCATATTTC
          661 AAGTTTCTGA ATGTTTCCCT GAGGTTTGCT GCATCGACAT CTGTACCAGA CCGAGATGTC
          721 ATTCCAGTGC TTTTATGAAA ATTCTTATTA TTAATTTATT ATACATAAAC CCAATCTCAG
          781 GATAATCCAT TTTATAAACT GTTGTCCAGG GATATTCCAG AGTCCATTGA TTCGCTTCCA
          841 TGTATGATCT TTGGGTTCCA AAATTTTTAA TGGATTTTGA ATCCACTGAA GTTTTCAGTG
          901 TTCTCCATTG GGTACCTGCT GGGAATACTG TAACTGTGCT TTGAGGACTT GCGCTTTCAA
          961 GGGCTTGTCG GAACTGCTTT TCTAATGGTG ACAACTTGAT CCAAGGAAAC CGTAAACTTG
         1021 GAAAGTCTTC CATCCCATTT GGGAATATAA GGAAGCCTAA ACATTCTCCC AAGTTCTAAA
         1081 TCCTGTGTCC CTTGAAGTAC AAAATGGGTT AGAATCTTTC TGCCGAACCT TGCCGCAACG
         1141 GATCTTACGA CCATATAGTG AATCCTCCTT CCTCCTTCCT GTCTAGGCCG ACCGCTCAAG
        1201 TGTTCTACAG ATCTGATGGA GTA
        //
        Primer: M13_-40
        Sequence file: RDB14403_A6Kpd.seq check
        >14403_14403_A6Kp_1_M13_-40_B03_06_ABI24.ab1
            1 ACCGCCAGTC ATCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
           61 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
          121 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
          181 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
          241 GCTAGCGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC
          301 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC
          361 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC
          421 ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC
          481 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT
          541 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC
          601 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA
          661 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC
          721 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA
          781 GAGACATCAA AGAAATAACG CCGGAAACAT TAGTGCAGGC CAGCTTCCAA CAAGCAATGG
          841 CATCCCTGGT CATCCCAGCG GGATAGTTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA
        901 AGATTGTGGC ACCGCCCGCT TTACAGGCTT CGACGCCGCC TTTCGTTCTA ACCATC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl