Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG3

Retroviral expression vector of mouse Immunoglobulin G3 (IgG3).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14503_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14503_A6Lea.seq
>D04073A3_A6Le_1_pBABE5'_A06_03_ABI24.ab1     1 GCGATATGGA CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC    61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC   121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA   181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT   241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC   301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC   361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCCAGTGTGG TGGTACGGGA ATTCACACAG   421 GACCTCACCA TGGGATGGAG CTGTATCATG CTCTTCTTGG CAGCAACAGC TACAGGTGTC   481 CACTCCCAGG TCCAACTGCA GCAGCCTGGG GCTGAGCTTG TGAAGCCTGG GGCTTCAGTG   541 AAGCTGTCCT GCAAGGCTTC TGGCTACACC TTCACCAGCT ACTTGATGCA CTGGGTGAAG   601 CAGAGGCCTG GACGAGGCCT TGAGTGGATT GGAAGGATTG ATCCTAATAG TGGTGGTACT   661 AAGTACAATG AGAAGTTCAA GAGCAAGGCC ACACTGACTG TAGACAAACC CTCCAGCACA   721 GCCTACATGC AGCTCAGCAG CCTGACATCT GAGGACTCTG CGGTCTATTA TTGTGCAAGA   781 TATGATTACT ACGGTAGTAG CTACTTTGAC TACTGGGGCC AAGGCACCAC TCTCACAGTC   841 TCGAGTGCTA CAACAACAGC CCCATCTGTC TATCCCTTGG TCCCTGGCTG CGGTGACACA   901 TCTGGATCCT CGGTGACACT GGGATGCCTT GTCAAAGGCT ACTTCCCTGA GCCGGTAACT   961 GTAAAATGGA ACTATGGAGC CCTGTCCAGC GGTGTGCGCA CAGTCTCATC TGTACTGCAG  1021 TCTGGGATTC TATTCCCCTC AGCAGCTTGG TGACTGTACC CCTCCAGCAC CTGGCCCCAG 1081 CAGACTGGTC AATCTGCCAA CGTAGCTCCA CCCCAGGCCA GCCAAAGG //
Primer: IRES-R Sequence file: RDB14503_A6Leb.seq
>D04073A3_A6Le_1_IRES-R_C02_08_ABI24.ab1     1 GCGTCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA    61 ATTTACGTAG CGGCCGCCTA GGCACCTTGT CCAATCATGT TCCTGTAGTC AGGGATGGCC   121 GTCTGCTTCA CCTGCACCAC TGAGGAGAAG ATCCACTTCA CCTTGAAGAG GGTGACAGAG   181 GCGCTGTAGC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT CGTCCAGAGC   241 CCGTCCAGCT CCCCATCCTG GGCCTCAGCA CAGGTCTCAT TCAGTTCCAG CTCAGGGGAG   301 CGAGACAGGT TCTTCTGTGT GTGGTGGTTA TGGAGAGCCT CATGCACCAC GGAGCAGGTA   361 AAAATTTCTC CTTGCAACCA ACTGTCTGTA TCCACAGTGA GCTTGCTGTA GAGGAAGTAG   421 GTCCCATCCG AGTCCAGGAT GGGTGGAGTG TTCTTGTAAT CCTGCTCCAG TTCTCCGTTC   481 CTTTCCCACT CCACACTGAT GGCTTCAGAG AAGAAGTTGG TGACCAGGCA GGTCAAACTA   541 ACCTTCTTCT TGGACATTTG TTCACGAGGT GGGGGTATGG TGTATACTTG AGGTGTCTGG   601 GCTCTTCCTT TGGGTTTTGA GATGGTTCTC TCGATGGGGG CTGGGAGGGC TTTGTTGTTG   661 ACCTTGCATT TGAACTCCTT GCCCCTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG   721 ACCACTCGGA AGGTACTGTT GTACTGAGCT TCACGGGGCT GCGTCCAGGC TGTGTGTACT   781 TCTTTGTTGT CCACAAACCA GCTGACATGG ACATCTGGGT CATCCTCGCT CACATCCACC   841 ACCACACACG TAACCTTGGG GGTTAGGGAG ATCATGAGTG CATCCTTGGG CTTTGGGGGG   901 GAAGATGAAG ACGGATGGTC CACCCAAGAT GTTACCAGGG TGGGCATGAA GAACCCTGGG   961 GGGGGTACTG GGCTTGGTAT TCTAGGCTCG ATTCTCTTGA TCAACTCAGT CTGGCTGGCT  1021 GGGTGGCTAC GGTGGCAGAT GGACAGTCTG CTTGATAGTT GCTTGAGGTA CAGTCACAGC  1081 TGCTGAGGAA TAGACCAGAC TGCAGTACGA TGAACTGTGG CGCACACGCT GGACAAGGGC 1141 CTTCCCT //
Primer: IRES-F Sequence file: RDB14503_A6Lec.seq
>D04073A3_A6Le_1_IRES-F_C06_09_ABI24.ab1     1 CTTCAAACCT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG    61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG   121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC   241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC   301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT   361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG   421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG   481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA   541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA   601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC   661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC   721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG   781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGGCCGC   841 CAGCACAGTG GTCGACGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGGA   901 ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTTGCAAGGG   961 CATGGGAAAA ATACATAACT GAGAATAGAG AAAGTTCCAG GATCAAGGTC AGGAACAGGA  1021 TGGGAACAGC CTGAATATGG GGCCAACAGA TATCTGTGGT AGCAGTTCCT TGCCCCGGCT 1081 CAAGGCTAGG ACCAGAGTGG CAACAGCCTG AAATATAATT GGGACCTCAA AC //
Primer: Amp_R Sequence file: RDB14503_A6Led.seq
>D04073A3_A6Le_1_Amp_R_D06_12_ABI24.ab1     1 CTCGTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG    61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG   181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA   241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC   301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG   361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA   421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC   481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG   541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT   601 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG   661 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT   721 TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG   781 GTAAGCTGGC CAGCAACTTA TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT   841 ATGCGCCTGC GTCGGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA   901 ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCTGGGGC   961 CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTTGGGACT CTTTGGGTGC  1021 AACCCCCCCT TTAGAAGAGG ATATGTTGGA TCCTTGTTAA GAGACGAAGG AACTAAACAG 1081 ATCCGGCCTT CGATCCTGAA GTTTTTGGCA TTTTCCAGT //
Primer: SV40pA3'element Reverse Sequence file: RDB14503_A6Lee.seq
>D04073A3_A6Le_1_SV40pA3element Reverse_E06_15_ABI24.ab1     1 GTCGCAGAGG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT    61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC   121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG   181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT   241 GGCCGATTCA TTAATGCAAC TATGGCCATT TAATGTAAAT ACTTAAGAAA AAAAACCAAA   301 TTAATTTTGA TACATGCTGC ATGTGAAAGA CCCCCGCTGA CGGGTAGTCA ATCACTCAGA   361 GGAGACCCTC CCAAGGAACA GCGAGACCAC AAGTCGGATG CAACTGCAAG AGGGTTTATT   421 GGATACACGG GTACCCGGGC GACTCAGTCA ATCGGAGGAC TGGCGCCCCG AGTGAGGGGT   481 TGTGGGCTCT TTTATTGAGC TCGGGGAGCA GAAGCGCGCG AACAGAAGCG AGAAGCGAAC   541 TGATTGGTTA GTTCAAATAA GGCACAGGGT CATTTCAGGT CCTTGGGGCA CCCTGGAAAC   601 ATCTGATGGT TCTCTAGAAA CTGCTGAGGG CTGGACCGCA TCTGGGGACC ATCTGTTCTT   661 GGCCCTGAGC CGGGGCAGGA ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT   721 GTTCCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA TATCCTGTTT   781 GGCCCATATT CAGCTGTTCC ATCTGTTCCT GACCTTGATC TGAACTTCTC TATTCTCAGT   841 TATGTATTTT TTCCATGCCT TGCAAAATGG CGTTACTTAA GCTAGCTTGC CAAACCTACA   901 GGTGGGGGTC TTTCATTCCC CCCCTTTTTT CTGGAGACTA AATAAAATCT TTTTATTTTT   961 ATCGTCGACC CACTGTGCTG GGCGGCCCGG CCCGCTTTTA CTTTGTACAG CTCGTCCATG  1021 CCGAGAGTTG ATCCCGGCGC GGTCACGAAC TCCAAGCAGA CCATGTGATT CGCGCTTCTC 1081 GTTGGGGATC TTTGGCTCAG GTCGGAACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl