Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG2c

Retroviral expression vector of mouse Immunoglobulin G2c (IgG2c).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14506_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14506_A6Lea.seq
>D04073A6_A6Le_1_pBABE5'_D01_10_ABI24.ab1     1 GTTTTATATG GCCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG    61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT   121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC   181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA   241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG   301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC   361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCACAC   421 AGGACCTCAC CATGGGATGG AGCTGTATCA TGCTCTTCTT GGCAGCAACA GCTACAGGTG   481 TCCACTCCCA GGTCCAACTG CAGCAGCCTG GGGCTGAGCT TGTGAAGCCT GGGGCTTCAG   541 TGAAGCTGTC CTGCAAGGCT TCTGGCTACA CCTTCACCAG CTACTTGATG CACTGGGTGA   601 AGCAGAGGCC TGGACGAGGC CTTGAGTGGA TTGGAAGGAT TGATCCTAAT AGTGGTGGTA   661 CTAAGTACAA TGAGAAGTTC AAGAGCAAGG CCACACTGAC TGTAGACAAA CCCTCCAGCA   721 CAGCCTACAT GCAGCTCAGC AGCCTGACAT CTGAGGACTC TGCGGTCTAT TATTGTGCAA   781 GATATGATTA CTACGGTAGT AGCTACTTTG ACTACTGGGG CCAAGGCACC ACTCTCACAG   841 TCTCGAGTGC CAAAACAACA GCCCCATCGG TCTATCCACT GGCCCCTGTG TGTGGAGGTA   901 CAACTGGCTC CTCGGTGACT CTAGGATGCC TGGGTCAAGG GGTTATTTCC CTGAGCCAGT   961 GACCTTGACC TGGAACTCTG GGATCCCTGT CCAGTGGTGT GCACACCCTT CCCAGCTCTC  1021 CCTGCAGTCT GGGCCTCTAC ACCCTCAGCA GCTTCAGTGA CTGTAAACCT CGAACACCTG  1081 GCCCAGCCAG ACAATCACCT GCAATGTGGC CACCGGCCAG CAGCAACAAG TGGACCAAGA 1141 AAAATTGGAA GCCCAGAGGG TGCCCCATT //
Primer: IRES-R Sequence file: RDB14506_A6Leb.seq
>D04073A6_A6Le_1_IRES-R_F02_17_ABI24.ab1     1 GCATAGCGGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA    61 ATTTACGTAG CGGCCGCCTA GGCTCCCTGC CCAATCATGT TTCTGTAGTC AGGGGAGATC   121 TTCTGCTTCA GCTCCACCAC AGAGGAGAAG ATCCACTTTA CCTTGAAGAG TGTGACAGAG   181 GCGCTGTAAC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT TGTCCAGAGC   241 CCGTCCAGCT CCCCATCCTG GGCCTCAGTA CAGACATCAT CCAGGTCTAG CCCCAGAGAC   301 CGGGAGATGG TCTTAGTCGT AAGGTGATTG TGCAGACCCT CGTGGACCAC TGAGCAGGCG   361 AAAAGACTTC CTCTTTCCCA AGTGCTCTTT TGTACTCTGA GCTTGCTGTA CATGAAGTAA   421 GAACCATCAA AGTCCAGGAC TGTTGCGGTG TTCTTGTAGT TTTGCTCTGT ACGCCCATTG   481 CTGGTCCAGT CCACAGCAAT TTCGGCAGGT AAGAAGCCTG TGATCATGCA GGTCAGACTG   541 AACTCTTTCT TAGTCATCTC TTCTGCTGGT GGAGGCAAGA CATATACCTG TGGAGCTCTT   601 ACTGGCCCTC TGGGTTTTGA GATGGTTTTC TCGATGGGGG ATGGGAGGGC TCTGTTGTTG   661 ACCTTGCATT TGAACTCCTT GCCACTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG   721 ACCACCCGGA GAGTACTGTT GTAATCCTCT CTATGGGTTT GTGTCTGAGC TGTGTGTACT   781 TCCACGTTGT TCACAAACCA GCTGATCTGG ACGTCTGGGT CATCCTCGCT CACATCCACC   841 ACCACACATG TGACCATGGG GCTCAGGGAG ATCATGAGTA CATCCTTGAT CTTTGGAGGG   901 AAGATGAAGA CGGATGGTCC ACCCAAGAGG TCTGGAGCTG CGCATGGGGG ACACTCTTTG   961 AGTGGAGGAC AGGGGTTCTG TGTTATGGGC ACTCTGGCTC AATTTTCTTG TCCACTTTTG  1021 GTGCTGCTTG CCGGTGGGCC ACATTGGCAG TGATGTCTGC TGGGCCAGTG TCGAAGCTAC  1081 AGTCACTGAG CTGCTGAGCT GTAAGCCAGA CTGCAGGAAG CTTGAGTGTG CACACCCACT 1141 TGGACACAG //
Primer: IRES-F Sequence file: RDB14506_A6Lec.seq
>D04073A6_A6Le_1_IRES-F_C09_09_ABI24.ab1     1 GGCAGTCTTA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA   121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA   181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA   241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC   301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA   361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA   421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT   481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA   541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT   601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA   661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC   721 CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC   781 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC GGCCGCCAGC   841 ACAGTGGTCG ACGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAACG GGGGGAATGA   901 AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA   961 AAAATACATA AACTGAGAAT AGAGAAGTTT CAGATCAACG TCAGGAACAG ATGGGAACAG  1021 CTGATATGGG CCAAACAGGG ATATCTGTGT TAGGCAGATC TGCCCCGGGC TCAGCTAGAC 1081 AGATGGAACA GCTGATTATG GACCAAACCA TGA //
Primer: Amp_R Sequence file: RDB14506_A6Led.seq
>D04073A6_A6Le_1_Amp_R_D09_12_ABI24.ab1     1 CTTCGTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT   181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG   241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG   601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC   661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT   721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA   781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT   841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG   901 AACTGACGAG TTCGGAACAC CCGGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCTGGG   961 GCCGTTTTTT GTGGCCCGAC CTGAGTCCAA AAATCCCGAT CGTTTTGGAC TTCTTTGGTG  1021 CACCCCCCCT TAGAGAAGGG ATATTGTGGA TTCTGTTAGA GACCGAGACT AAACCAGTCG 1081 CTTCGATCTG AATTTTGCTT CCGATTGGGG AACCGAC //
Primer: SV40pA3'element Reverse Sequence file: RDB14506_A6Lee.seq
>D04073A6_A6Le_1_SV40pA3element Reverse_E09_15_ABI24.ab1     1 GTCGTCGGGG GGGCGGAGCC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG    61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT   121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC   181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG   241 CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT AAATACTTAA GAAAAAAAAC   301 CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG CTGACGGGTA GTCAATCACT   361 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT   421 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG   481 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC   541 GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC AGGTCCTTGG GGCACCCTGG   601 AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC CGCATCTGGG GACCATCTGT   661 TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC   721 AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT   781 GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT GATCTGAACT TCTCTATTCT   841 CAGTTATGTA TTTTTTCCAT GCCTTGCAAA ATGGCGTTAC TTAAGCTAGC TTGCCAAACC   901 TACAGGTGGG GGTCTTTTCA TTCCCCCCCC TTTTTTCTGG AGACTAAATA AAAATCTTTT   961 TAATTTTTAT CGTCGACCCA CTGTGCTGGG CGGGCCGGGC CGCTTTTACT TTGTACAGCT  1021 CGTCCCATGG CCGAGAGTGA TCCCCAGCCG GGCGGTCACG AACTTCCAGC AGGACCAATG 1081 TGAATCGCGC TTCTCGATTG GGGGATCTTT TGGCCTCCAA TGGGTCCGGG A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl