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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgA

Retroviral expression vector of mouse Immunoglobulin A (IgA).

Catalog number RDB14507
Resource name pMXs-IRES-GFP_IgA
Clone info. Retroviral expression vector of mouse Immunoglobulin A (IgA), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Igha cDNA
Depositor|Developer Kitamura, Daisuke |
Other clones in our bank

External Database
mouse Igha

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) を引用し、寄託者への謝辞の表明を必要とする。
          付加的提供条件:
          pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14507 pMXs-IRES-GFP_IgA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14507_A6Lep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14507_A6Lea.seq check
          >D04073A7_A6Le_1_pBABE5'_A10_01_ABI24.ab1
              1 GCGATATGGA CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC
             61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC
            121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA
            181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
            241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC
            301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
            361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCCAGTGTGG TGGTACGGGA ATTCACACAG
            421 GACCTCACCA TGGGATGGAG CTGTATCATG CTCTTCTTGG CAGCAACAGC TACAGGTGTC
            481 CACTCCCAGG TCCAACTGCA GCAGCCTGGG GCTGAGCTTG TGAAGCCTGG GGCTTCAGTG
            541 AAGCTGTCCT GCAAGGCTTC TGGCTACACC TTCACCAGCT ACTTGATGCA CTGGGTGAAG
            601 CAGAGGCCTG GACGAGGCCT TGAGTGGATT GGAAGGATTG ATCCTAATAG TGGTGGTACT
            661 AAGTACAATG AGAAGTTCAA GAGCAAGGCC ACACTGACTG TAGACAAACC CTCCAGCACA
            721 GCCTACATGC AGCTCAGCAG CCTGACATCT GAGGACTCTG CGGTCTATTA TTGTGCAAGA
            781 TATGATTACT ACGGTAGTAG CTACTTTGAC TACTGGGGCC AAGGCACCAC TCTCACAGTC
            841 TCGAGTGAGT CTGCGAGAAA TCCCACCATC TACCCACTGA CACTCCCACG AGCTCTGTCA
            901 AGTGACCCAG TGATAATCGG GCTGCCTGAT TCACGATTAC TTCCCTTCCC GGCACGATGA
            961 ATGTGACCTG GGGAAAAGAG TGGAAGGATA TAACCACCGT AAACTTCCCA CCTGCCCTGG
           1021 GCCTCTGGGG GAGGTACACC CATGAAGCAG CCAGCTGACC CTGCCAGCTG TCGGAGTGCC
          1081 AGAAGAGATT CGTGCAATGT TTCGGTGCAA ACAATGAGAC CTCCCT
          //
          Primer: IRES-R
          Sequence file: RDB14507_A6Leb.seq check
          >D04073A7_A6Le_1_IRES-R_G02_20_ABI24.ab1
              1 GCATCAGCGG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
             61 ATTTACGTAG CGGCCGCTCA GTACTGGGGG ACCTCTTTGC TGCCAAACGG GCCTCGAACA
            121 GTTGTAACAG TGAGTGCTGT GCTGTAGAAC AAGCTCAGTA GGAAGAGGGT GAGGAAGGTC
            181 ACAGTGGTGG GCCACAGGCT GGCACCTGGG GCCTCTTCCT CCAGGATGTC TTCTGACTGG
            241 TCCAGTAGCA CATAGGAAAG TGGCTCTTGA CGTTCCGACA GACGGTCGAT GGTCTTCTGG
            301 GTGAAGTTCA TGGGCAAGGC CTCGTGGCCC ACCATGCAGG AGTACTGGTC ACCCTGTTTC
            361 CAGAGTTCAG CTGATACACG CAACACGCTT GTCACCAGGT AGGTGGTGGC TCCCTCGCCT
            421 GGCTCCTTTA GGGGCTCAAA CACTAGGTAG CTTTCTGGGG ACAGCTCCTC ATTTCCATGC
            481 AGCCATCGCA CCAGCACTTC TTTAGGGTTG AAAGCTCGCA CCAGGCATGT CAGGGACACG
            541 AGCTCATTCA GGGCCAGCTC CTCCGACGGC GGCGGTAGCA GGTGGACCTG GGGTGGGAAG
            601 GTGTTCACTG TGATTTTGGC AATTGTGCCA GTTAAGGTGT CAGACTCAGG ATGGGTAACT
            661 GTGCACTTGA ATGATGCGCC ACTGTTCCAG CGCTCAGCAC AGCCAGGCAG GACGCTGGAC
            721 ACACTGTAGC AGCCGCAGGA ATTCTGCACA GCTTTCTTCT GCACTGCATC CTTCCCAGTG
            781 GAGGGCTCCC AGGTGAAGAC AGCTCCCTCA GGATTTCTCA GGCCATTCAG AGTACATGTG
            841 AGGCTGGCAT CTGAACCCAG GAGCAGGTCC TCAAGAGCTG GCCGCTGCAG TGACAGGCTG
            901 GGATGGCAGG AAGGAGGACA AGGAGGACAA GGAGGAGGAG GACCAGAGCA CTTCACATCC
            961 AATTCTTGGA CGGCGTTAGA GTCATGTTGC ACGGAACATT TCACGGGATT CTCCTTCTGG
           1021 CACTCGACAG CTGGCAGGTC AACTGCTGCT CATGGTGTAC CCTCCCTCCA GAGCAAGGCA
           1081 AGTGAGGTTA CGTGTATATC GTTCCACTCT TTCCCAAGTC ACATTCATCG TTGCCGGAAA
          //
          Primer: IRES-F
          Sequence file: RDB14507_A6Lec.seq check
          >D04073A7_A6Le_1_IRES-F_C10_07_ABI24.ab1
              1 GGAAAGGCTT TATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
             61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
            121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
            181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
            241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
            301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
            361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
            421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
            481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
            541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
            601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
            661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
            721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
            781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
            841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTTATTTAGT CTCCAGAAAA AGGGGGGGGA
            901 ATGAAAGACC CCCACCTGTA GGTTTTGGCA AGCTAGCTTA AAGTTAACGC CCATTTTTGC
            961 AAGGGCATGG AAAAAATACA TTAACTTGAG AAATTAGAGA AAGTTTCAGA TTCACGGTTC
           1021 AGGACAGATG GGAAACAGCT GAATATGGCC AACAGGATTA TCTTGTGGTA AGCAGTTCCT
          1081 TGCCCCCGCC TCAGCTAGAA CAGATGGAAC AAGCCTTGAA TTATTGGGGC CCCA
          //
          Primer: Amp_R
          Sequence file: RDB14507_A6Led.seq check
          >D04073A7_A6Le_1_Amp_R_D10_10_ABI24.ab1
              1 CTCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
            181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
            241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
            301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
            361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
            421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
            481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
            541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
            601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
            661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
            721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
            781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
            841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
            901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG
            961 GCCGTTTTTG TGGCCCGACC TGAGTCCAAA ATCCCGATCG TTTTGGGACT CTTTGGTGCA
           1021 CCCCCCTTAG AAGGATGGAT ATGTGATTCT GGTTAGGAGA CCGAGATCTA AACAGTACTC
          1081 CTTCCGTCTG AATTTTTTGC CTTTCCGCTT TGGGGAACCG AGC
          //
          Primer: SV40pA3'element Reverse
          Sequence file: RDB14507_A6Lee.seq check
          >D04073A7_A6Le_1_SV40pA3element Reverse_E10_13_ABI24.ab1
              1 GTTCGCTGGG GGGGCGGGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
            121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
            181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
            241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
            301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
            361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
            421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
            481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
            541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
            601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
            661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
            721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
            781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
            841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
            901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
            961 TTTATCGTCG GACCCACTGT GCTGGGCGGG CCCGGCCGCT TTACTTGTAC AGCTCGTCCA
           1021 TGCCCGAGAG TGATCCCAGC GGCGGTCACG ACCTCAGCAG ACCATGTGAA TCGCCGCTTC
          1081 TTCGCTGGGA TCTTGGCTTC AACGCTGACT GGAATGCTTC AAGGCTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl