Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgE

Retroviral expression vector of mouse Immunoglobulin E (IgE).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14508_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14508_A6Lea.seq
>D04073A8_A6Le_1_pBABE5'_A11_02_ABI24.ab1     1 GCGAATATGG ACATATGAGA TCTTATATGG GGCACCCCCG CCCCTTGTAA ACTTCCCTGA    61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA   121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG   181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC   241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC   301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC   361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCCAGTGTG GTGGTACGGG AATTCACACA   421 GGACCTCACC ATGGGATGGA GCTGTATCAT GCTCTTCTTG GCAGCAACAG CTACAGGTGT   481 CCACTCCCAG GTCCAACTGC AGCAGCCTGG GGCTGAGCTT GTGAAGCCTG GGGCTTCAGT   541 GAAGCTGTCC TGCAAGGCTT CTGGCTACAC CTTCACCAGC TACTTGATGC ACTGGGTGAA   601 GCAGAGGCCT GGACGAGGCC TTGAGTGGAT TGGAAGGATT GATCCTAATA GTGGTGGTAC   661 TAAGTACAAT GAGAAGTTCA AGAGCAAGGC CACACTGACT GTAGACAAAC CCTCCAGCAC   721 AGCCTACATG CAGCTCAGCA GCCTGACATC TGAGGACTCT GCGGTCTATT ATTGTGCAAG   781 ATATGATTAC TACGGTAGTA GCTACTTTGA CTACTGGGGC CAAGGCACCA CTCTCACAGT   841 CTCGAGTTCT ATCAGGAACC CTCAGCTCTA CCCCTTGAAG CCCTGTAAAG GCACTGCTTC   901 CATGACCCTG GGCTGCCTGG TAAAGGACTA CTTCCCTGGT CCTGTGACTG TGACCTGGTA   961 TTCAGACTCC CTGAACATGA GCACTGTGAA CTTCCCTGCC CTTGGATTCT GAACTCAAAG  1021 TCACCACCCA GCCAAGTGAC CAGCTGGGGC AAGGTTCAGC TAGGACTTTC ACATGGCCAC 1081 GTGACACATC GTTCATTCAA TCCACGAAAG TAGGAACTTA ATCCTTAGAT CG //
Primer: IRES-R Sequence file: RDB14508_A6Leb.seq
>D04073A8_A6Le_1_IRES-R_H02_23_ABI24.ab1     1 GCATCAGCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG    61 GAATTTACGT AGCGGCCGCC TATGCCCTGG TCTGGAAGAT GTTGGCATAG TCTTGGAAGG   121 TCTGGGGTGT ATCCTGCATC GGTGTGGAGA AGACCCACTT CACCTTGAGG ACGGTGACAG   181 TGGCCCCATA GCTCACACTG AGCAGGAACA GGGTGATGAA GACACAAATA CTGGTCCACA   241 GCTCTTCCAG CTCCTCGCCC TCCACCTCTT CAATACATAG GTCCTGGAGG TCTAGCTCAA   301 GGCTCGTGGA TATTGTTTTC TCCAGTTTCC TGGGTTTCTG AAGTGCCTCA TGGATCACTT   361 GGCAGGTGAA CTGTTTTCTC TGTGTCCAGA GTGTCTTGGC GACCTCTAGG CGACTGAAGA   421 TGAAAAAGCC TTGATTGGAG CCATTGGATT TCAGGGGTGT TGTGGTACTG TGATGGCTGT   481 TTGAGATCAG TTTGCCATCC TCCAGCCACT GCACAGAGAT ATCCTCAGGG AAGAAGTTCT   541 GGATCAAGCA GGTGAGTGTG CGTTTGTCCT CGCTCTCCTC CTCTGGTGGT GGGAACACAT   601 ATACCTCGGG GGCTGAGCGC TGGCCTGGGG TCTTGGTGAT GGAACGCACA ATGGGCTTGG   661 GAAAATCAGG GTGGTCCACT ATGCACTGAT AGCCGTAGCC TTCAATCCAG TCCTTGGCAA   721 CTACAGGCAG GATGGAGGTG ATACTGGTTG TGGCGTTATG GTGGTGCTTA GTGTACCACT   781 GGGATGCTGA GACTGGAGTC TTCTTCTCTT GGTTCCACGT CACATTGACA TTCTTCTCGC   841 TTTCCAGGTC CACCACCAGA CAGGTAAGCT TGGGAGCACC GTTTTGATAC AGGTCCAGGG   901 GGCTGGGTGG GATCAGGTAG GTAATCACAC CCCGTGGCTC ATGATCTGGG CATCTCCGAG   961 TGTGGGCCAA ATAGTCTACG CCTTGGGGAG GTGACCTTGC AGGTGAAGGT GCTTTCAGAC  1021 ATCCATTGCT GCTCAGTGAT GTTGAGTTAC TGCAGTAGAG CTAGTTTGCT CCTCCTGAAT  1081 AGACAGTTGT GCAGTGTATC AGTTATCTTC GATCGTCATT AAGCCTAGCT GACCGAAGAA 1141 CATCCAATTT AGGATGTGTG //
Primer: IRES-F Sequence file: RDB14508_A6Lec.seq
>D04073A8_A6Le_1_IRES-F_C11_08_ABI24.ab1     1 GCCATTCCTT TCTGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA   841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGGAAT   901 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTTAAGT AACGCCATTT TTGCAAGGGC   961 ATGGAAAAAT ACATAACTGA GAATAGAGAA AGTTCAGATC CAAGGGTCAG AACAAGATGG  1021 GAACAGCCTG AATATGGGCC AACAGGATAT CTGTGGGTAG GCAGTTCCTG CCCCGGCTCA 1081 AGCAGAACCA GATGAACAGC TGGAATATGG GTCCAAAACA AGGGAT //
Primer: Amp_R Sequence file: RDB14508_A6Led.seq
>D04073A8_A6Le_1_Amp_R_D11_11_ABI24.ab1     1 CTTTTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT   181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG   241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG   601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC   661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT   721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA   781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT   841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG   901 AACTGACGAG TTCGGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTCGGGGG   961 GGCCGTTTTT GTGGGCCCGA CCTGAGTCCC AAAAAATCCC GATCGTTTTG GACTCTTTTG  1021 GTTGCACCCC CCCTTAGAGG AGGATATGTT GGTTCCTGTT AAGAGACGAG AACTAAACAG 1081 TCCGGCCTTC GTCTGGATTT TTGGCCTTTC GGTTTTTGGG GAACCCGA //
Primer: SV40pA3'element Reverse Sequence file: RDB14508_A6Lee.seq
>D04073A8_A6Le_1_SV40pA3element Reverse_E11_14_ABI24.ab1     1 GTAGGTTTGG GGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT    61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG   121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG   181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC   241 CCGCGCGTTG GCCGATTCAT TAATGCAACT ATGGCCATTT AATGTAAATA CTTAAGAAAA   301 AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC CCCCGCTGAC GGGTAGTCAA   361 TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA   421 GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCCCCGA   481 GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA   541 GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCAGGTC CTTGGGGCAC   601 CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC TGGACCGCAT CTGGGGACCA   661 TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT GTTTGGCCCA   721 TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGG CAGGAACTGC TTACCACAGA   781 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCCT GACCTTGATC TGAACTTCTC   841 AACCCCCCCT CCCACTCCAC ATAGAGATTT TTTTCCCCCC CCCGCGAAAA AAGGGGGGTT   901 ATATATATAC AAAGCTGCCA GGCGGGGGGT CGTTTCGGTG TCCCCCCCTT TTTTTCCTGG   961 GAGAACTAAA TAAAAATCCT TTTTATTTTT TATCCGTTCG ACCAACTGTT GCCTGGGCGG  1021 GCCGGGCCCG CCTTTTACCT TTGTACCAGC CTTCGGTTCC CAATGGCCCG AGAAGGTGGA  1081 ATCCCCCAGG CCGGGCGGGG TCCACCGGAA CTTCCAATCA GGAACCCCAT GGGTGAATCC 1141 GCCGGCTTTC TCTCCGTTTG GGGGGGACCT TTGTGCCCTC TCACGGGGGC CTCGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl