Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-mCherry_IgE

Retroviral expression vector of mouse Immunoglobulin E (IgE).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions for distribution	Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
付加的提供条件	pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14510_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14510_A6Lea.seq
>D04073B1_A6Le_1_pBABE5'_F04_16_ABI24.ab1     1 GTCAATATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC    61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC   121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA   181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT   241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC   301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC   361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCCAGTGTGG TGGTACGGGA ATTCACACAG   421 GACCTCACCA TGGGATGGAG CTGTATCATG CTCTTCTTGG CAGCAACAGC TACAGGTGTC   481 CACTCCCAGG TCCAACTGCA GCAGCCTGGG GCTGAGCTTG TGAAGCCTGG GGCTTCAGTG   541 AAGCTGTCCT GCAAGGCTTC TGGCTACACC TTCACCAGCT ACTTGATGCA CTGGGTGAAG   601 CAGAGGCCTG GACGAGGCCT TGAGTGGATT GGAAGGATTG ATCCTAATAG TGGTGGTACT   661 AAGTACAATG AGAAGTTCAA GAGCAAGGCC ACACTGACTG TAGACAAACC CTCCAGCACA   721 GCCTACATGC AGCTCAGCAG CCTGACATCT GAGGACTCTG CGGTCTATTA TTGTGCAAGA   781 TATGATTACT ACGGTAGTAG CTACTTTGAC TACTGGGGCC AAGGCACCAC TCTCACAGTC   841 TCGAGTTCTA TCAGGAACCC TCAGCTCTAC CCCTTGAAGC CCTGTAAAGG CACTGCTTCC   901 ATGACCCTGG GCTGCCTGGT AAAGGGACTA CTTCCCTGGT CCTGTGACTG TGACCTGGTA   961 TTCAGACTCC CTGAACATGA GCACTGTGAA CTTCCCTGCC CTTTGGGTTC TGACTCAAGT  1021 CACCCACCCA AGCCCAAGTG ACCCAGCTGG GGGCAAGTCA GCCAAGGACT CACATGCCAC 1081 GTGGACACAT TCTTCAATCA ATTCCAACGA AGGTAAGGAA CTATTCCCTA GTTTCGGA //
Primer: IRES-R Sequence file: RDB14510_A6Leb.seq
>D04073B1_A6Le_1_IRES-R_B03_06_ABI24.ab1     1 GCGTCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA    61 ATTTACGTAG CGGCCGCCTA TGCCCTGGTC TGGAAGATGT TGGCATAGTC TTGGAAGGTC   121 TGGGGTGTAT CCTGCATCGG TGTGGAGAAA ACCCACTTCA CCTTGAGGAC GGTGACAGTG   181 GCCCCATAGC TCACACTGAG CAGGAACAGG GTGATGAAGA CACAAATACT GGTCCACAGC   241 TCTTCCAGCT CCTCGCCCTC CACCTCTTCA ATACATAGGT CCTGGAGGTC TAGCTCAAGG   301 CTCGTGGATA TTGTTTTCTC CAGTTTCCTG GGTTTCTGAA GTGCCTCATG GATCACTTGG   361 CAGGTGAACT GTTTTCTCTG TGTCCAGAGT GTCTTGGCGA CCTCTAGGCG ACTGAAGATG   421 AAAAAGCCTT GATTGGAGCC ATTGGATTTC AGGGGTGTTG TGGTACTGTG ATGGCTGTTT   481 GAGATCAGTT TGCCATCCTC CAGCCACTGC ACAGAGATAT CCTCAGGGAA GAAGTTCTGG   541 ATCAAGCAGG TGAGTGTGCG TTTGTCCTCG CTCTCCTCCT CTGGTGGTGG GAACACATAT   601 ACCTCGGGGG CTGAGCGCTG GCCTGGGGTC TTGGTGATGG AACGCACAAT GGGCTTGGGA   661 AAATCAGGGT GGTCCACTAT GCACTGATAG CCGTAGCCTT CAATCCAGTC CTTGGCAACT   721 ACAGGCAGGA TGGAGGTGAT ACTGGTTGTG GCGTTATGGT GGTGCTTAGT GTACCACTGG   781 GATGCTGAGA CTGGAGTCTT CTTCTCTTGG TTCCACGTCA CATTGACATT CTTCTCGCTT   841 TCCAGGTCCA CCACCAGACA GGTAAGCTTG GGAGCACCGT TTTGATACAG GTCCAGGGGG   901 CTGGGTGGGA TCAGGTAGGT AATCACACCC CGTGGCTCAT GATCTGGGCA TCTCCGAGTG   961 TGGGCCAAAT AGTCTACGCC TTGGGAGGTG ACCTTTGCAG TGAAGGTGCT TTCAGACATC  1021 CATTGCTGCT CAGTGATGTG AGTTTACTGC AGTAGAGCTA GTTGCCTCCT CCTGATAGAC  1081 AGTTGTGCAG TGTATCAGCT ATCTTCGAAT CGTCATAGCA GCCTGACGAA GACATCAATT 1141 TAAGGAATGG GTGGCTCAAT A //
Primer: IRES-F Sequence file: RDB14510_A6Lec.seq
>D04073B1_A6Le_1_IRES-F_H04_22_ABI24.ab1     1 GGCAAGTCTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG   121 AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT   181 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA   241 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC   301 TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG   361 ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG   421 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA   481 AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA   541 TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA   601 TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT   661 ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA   721 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC   781 ACTCCACTCT CGGCATGGAC GAGCTGTACA AGTAAGCGGC CCGATAAAAT AAAAGATTTT   841 ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGTTTGG CAAGCTAGCT   901 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA   961 TCAAGGGTCA GAACAGATGG GACAGCTGAA TATGGGCCCA AACAGGATAT CTGTGGTAAG  1021 CAGTTTCCTG CCCCGGCTTC AGATCAAGAC AGATGGACAG CCTGGATATG GGTCAAACAG  1081 ATATTCTGTG TTAGCCAGGT ACTTGCCGAC TCAGGTCAAG ACGATGACCC CAGATTGCCC 1141 GG //
Primer: Amp_R Sequence file: RDB14510_A6Led.seq
>D04073B1_A6Le_1_Amp_R_F05_17_ABI24.ab1     1 CTTTGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA   241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC   301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC   361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG   421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT   481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC   541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA   601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT   661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA   721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG   781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT   841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG   901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG   961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCCA AAAATCCCGA TCGTTTTGGG ACTCTTTGGG  1021 TGCACCCCCC CTTTAGAAGG AAGGATATGT GGGTTCTGTT AAGAGACGAA CACCTAACAG 1081 ACCCGGCCTT CGGTCTGGAG TTTTTGCTTT CGGCTTTGGG GAACTGA //
Primer: SV40pA3'element Reverse Sequence file: RDB14510_A6Lee.seq
>D04073B1_A6Le_1_SV40pA3element Reverse_G05_20_ABI24.ab1     1 GAGTCAGGGG GGCGGACCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA   301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA   361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA   421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG   481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA   541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA   601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC   661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG   721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT   781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA   841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA   901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAT CTTTTTATTT   961 TTATCGGGCC CGCTTTACTT TGTACAGCTC GTCCCATGCC CGAGAGGTGG AGTGGCGGGC  1021 CCCTCCGGGC GGCGTTTCGT ACTGTTTCCC ACGATTGGTT GTTAGTTCCT TCGATTGTGG 1081 GAGTTGAATG ATCACTTGGA ATTGTTGAAC GATTGTTAAA CGTCGTCCCG T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl