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pAcGFP1-N1-Dsup

Expression vector of GFP-fused Ramazzottius varieornatus Dsup.

Catalog number RDB14552
Resource name pAcGFP1-N1-Dsup
Clone info. Expression vector of GFP (AcGFP)-fused Ramazzottius varieornatus Dsup under the CMV promoter for transient expression in mammalian cells.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAcGFP1-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus Dsup cDNA
Reference of insert sequence LC050827 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu | Hashimoto, Takuma |
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External Database
Ramazzottius varieornatus Dsup
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016)。
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            RDB14552 pAcGFP1-N1-Dsup Under QC test. Please contact us. DNA solution

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            How to cite this biological resource

            Materials & Methods section:

            The pAcGFP1-N1-Dsup was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14552).

            Reference section:

            Hashimoto, T., Horikawa, D.D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin-I, T., Minakuchi, Y., Ohishi, K., Motoyama, A., Aizu, T., Enomoto, A., Kondo, K., Tanaka, S., Hara, Y., Koshikawa, S., Sagara, H., Miura, T., Yokobori, S., Miyagawa, K., Suzuki, Y., Kubo, T., Oyama, M., Kohara, Y., Fujiyama, A., Arakawa, K., Katayama, T., Toyoda, A., Kunieda, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Hashimoto, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274. [link to RRC of NBRP]

            2023.12.13

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