RDB14569 - pCMV-HA-Bach2 wt

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pCMV-HA-Bach2 wt

Expression vector of HA-tagged mouse Bach2, wild type.

Catalog number	RDB14569
Resource name	pCMV-HA-Bach2 wt
Clone info.	Expression vector of HA-tagged mouse Bach2, wild type.
Comment	Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone	pCMV-HA (plasmid)
Size of vector backbone	3.8 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Bach2 cDNA
Depositor\|Developer	Yamashita, Masakatsu \|

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mouse Bach2

External Database
Mouse Bach2

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14569	pCMV-HA-Bach2 wt	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14569_A7B6p1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14569_A7B6a.seq
>D04207A1_A7B6_1_CMV Forward_A12_01_ABI08.ab1 1 ACGTTCAACT CCGGCCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT 121 TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC 181 TAGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC 241 AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT 301 ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG 361 GGCCCACCAT GTACCCATAC GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA 421 TTCGGTCGAC CATGTCTGTG GATGAGAAGC CTGGCTCCCC CATGTATGTA TATGAGTCCA 481 CAGTCCACTG TGCCAACATC CTCCTGGGCC TCAATGACCA ACGGAAGAAG GACATTCTCT 541 GTGACGTGAC GCTGATCGTG GAGAGGAAGG AGTTCCGAGC CCACCGGGCT GTGCTGGCCG 601 CATGCAGTGA ATACTTCTGG CAAGCACTGG TTGGACAGAC GAAAGATGAC TTGGTGGTCA 661 GCTTGCCTGA GGAGGTCACG GCCAGGGGCT TTGGGCCACT GCTACAGTTT GCCTACACTG 721 CCAAGCTGTT ACTCAGCAGA GAGAACATCC GGGAGGTCAT CCGCTGTGCT GAGTTCCTGC 781 GCATGCACAA TCTGGAGGAC TCCTGCTTCA GCTTCCTGCA GACCCAGCTC CTGAACAGGG 841 AGGATGGCCT GTTTGTTTGC AGAAAGGACA GTGCGTGCCA GCGCCCCCAA GAGGGACCAT 901 GGGGAATTCT GCAGGGAGAA GAGGAGGAAG AAGAGGAAAC AATGGACTCA GAGACAGCTA 961 GGGATGGCTT GCGCCACAGA CCAGATGCTC CCAGACCCCA TCAGCTTTGA GGCCACTGCC 1021 ATCCCAGTAG CAGAGAAAGA GAAGTCCTGC TCCCTGAGTC TTGAGTACCC ACAGAACACC 1081 AGAACTCGAA GGTGCATTGA CCAGTACCCT AGGAATATTA //
Primer: pAxCALNLF1 Sequence file: RDB14569_A7B6b.seq
>D04207A1_A7B6_1_pAxCALNLF1_B12_02_ABI08.ab1 1 CACCAGGTTA TCTTTATCAT GTCTGGATCC CCGCGGCCGC CTAGGCATAA TCTTTCCTGG 61 GCTGTTCGTC CGTTGTACAC TTCTCAGTCA TTTCCTGGCA GAAGTCCACC GTCACAGACT 121 GGCTCCTGGC TTCTAGGGGA GGTCCTCGTT CTGAGAATGT TCCAGGGTCA CTGCCTTCCA 181 ACCTCCTCCC AGAGGTACAA TTCTCAGAGG TGTTGCTGGG CACCCAGGGG AGCCCGGGGG 241 GAGCCGCCCC TGGCTCCAGG CAGCAGGGTA CACTTCCTCC TACTGCACAC GGGGAGGGTG 301 CAAGGCTCTG CTCAACACCC ACAGGAGGAG GGTTAACACT GGCTCCAGGG AGATCCATGG 361 GTATGAGGAC AGGGCAGTAG CGGTGCAGGG CCTGGATCTG CTCTGGACTC TGGATGTCTC 421 GACAGACCTC CTGGGAGAGG CAGGAAAAGT TGTCCAGGAG CTCTCCCATG CACGCCTTCA 481 GGTGATTCCT CTCCGACAAC AGTTTTTCCT TCTCGCACAC CAGTTTCCGT ATTTCACATT 541 CCAAGTTCTG AATACAGTCC AGCTTCCTCT TTCGACAGCG CTGGGCTGCA ATGCGGTTCT 601 TACTCCGCCT CCGGATGTCG TGGATGAACT CCAACTGTTC TGAGGTTAGC TTGTGCATTT 661 TGATCATCAT CTGGAAGTCG TTCCTGGGAA GGTCTGTGAT CTGATCGACA GGAAAAGGAA 721 GTTTAACCTC CTGGCCCCTG TCCTGCACAG GACACGACTC ACTGTCTGCT TCCGAGAATG 781 ATCCAGACTC GTCACTGGAA TTGGTTCCGT AAGACTGCTC ACATTTAATC TGGGGTCGGA 841 CTTGGTTGTA CATTCCATCT CCCATCACGC CTGGTTCCTG ATGTTCCGTG GCAAGGGAAT 901 CTGGCTCCCT GGGAACAGGG CGAAGAGGAG AACTCACAGA GAGGAGGCTG CAGGGGGGGA 961 GCCCCCCACT CCCGTCCTCT GCGTAGGAAT ACGAGGAGCA AGAGCTGGAG GTCTAGTCCT 1021 GGTCTCGAGC GAGGTGAGCG GAGACAGACC TTGGATGGCA ACGGCAGCTT GTGATGGCCG 1081 TAATCCTACT TGCAGTTGAT CAGCCCATCC GAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl