Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-IP-GFP-LC3-RFP-LC3 delta G

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	(1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link]
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

提供条件	1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 付加的提供条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 寄託者による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 寄託者の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14600_A7Bap1-5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 (Pr0364) Region: insert 5'(EGFP 5') Sequence file: RDB14600_A7Bag.seq
>D04280A1_A7Ba_1_MMLV-pol_F2_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCC NCNNNNNTGC CGGNNCTCCC GCCGCCNCCA TGGTGAGCAA    61 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA   121 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC   181 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC   241 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT   301 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA   361 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT   421 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA   481 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT   541 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA   601 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC   661 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT   721 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGANCTG TACAAGGNTG GCGGTGGCTC   781 GNGCGGTGGT GNGTCGGGTG GCGGCGGNTC TATCGATGAA TTGAATTCCN TGCCGTCNGA   841 NAAGANCTTC NAACAGCGCC NGANCTNCNA ACAAAGANTG GAANANGNCC GGNTNNTNCN   901 GGNNCANCAC CCCNCCAANA TCCNNGNGNN TATANANCGA TACNNGGNNG ANAANCANCT   961 GCCNNNCNNN NNNNANNNNN TTNNNNGNNN NNGNNNNCGT NNNNNTGANC NNNNTCNTCN 1021 NNANNANTNN NNNNNNNN //
Primer: mRFP1-C_F (Pr0502) Region: insert 3'(rat LC3 deltaG),IRES Sequence file: RDB14600_A7Bah.seq
>14600_14600_B2FhF_1_mRFP1-C_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCTGGNNNTC NCCTCCCNCA ACGAGGACTA CACCATCGTG    61 GAACAGTACG AGCNCNCCNA GGGCCGCCAC TCCACCGGCG CCATCGATGA ATTGAATTCC 121 ATGCCGTCCG AGNNNNNNNN NN //
Primer: mVenus-M_F (Pr0574) Region: insert mid(EGFP 3',rat LC3,mRFP1 5') Sequence file: RDB14600_A7Bai.seq
>14600_14600_B2FhF_mVenus-M_F_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNGGCAT CNNGGTGNAC TTNNAGANCC GCCNCAACAT CGAGGACGGC    61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG   121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG   181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC   241 GAGCTGTACA AGGGTGGCGG TGGCTCGGGC GGTGGTGGGT CGGGTGGCGG CGGATCTATC   301 GATGAATTGA ATTCCATGCC GTCCGAGAAG ACCTTCAAAC AGCGCCGGAG CTTCGAACAA   361 AGAGTGGAAG ATGTCCGGCT CATCCGGGAG CAGCACCCCA CCAAGATCCC AGTGATTATA   421 GAGCGATACA AGGGTGAGAA GCAGCTGCCC GTCCTGGACA AGACCAAGTT CCTTGTACCT   481 GATCACGTGA ATATGAGCGA ACTCATCAAG ATAATTAGAA GGCGCCTGCA GCTCAATGCT   541 AACCAAGCCT TCTTCCTCCT GGTGAATGGG CACAGCATGG TGAGTGTGTC CACACCCATC   601 TCTGAAGTGT ACGAGAGCGA GAGAGATGAA GACGGCTTCC TGTACATGGT CTATGCCTCC   661 CAGGAGACGT TCGGGATGGC CTCCTCCGAG GANGTCATCA AGGAGTTCAT GCGCTTCAAG   721 GTGCGCATGG ANGGCTCCGT GAACGGNCAC GAGTTCGAGA TCGANGGNGA NGGCGANGGN   781 CGCCCCTACG AGGGCACCCA GACCGCCNAG CTGAANGTGA NCAANGGCGG NCCCCTGCCC   841 TTCNCCNGGG ACATCCNGNC CCCTCNGTTC CAGTACNGNT CCNAGGNCTA CGTGAAGCAN   901 CCCGCCNANN TCCCNNANNA CTTGAAGCTG TCCTTCCCCG ANGGCTTCNA GNNNNNNCNC   961 GNGNTGAACT TCGANGANNN NNNCNNNNNG ANNNNNNNCC NNNNTNNTNC CNGNNNNNNN 1021 NNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(mRFP1 3',rat LC3 deltaG 5') Sequence file: RDB14600_A7Baj.seq
>D04280A1_A7Ba_1_mRFP1-inner1_E10_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NTCCTCCCTG NNGGNNGGCG AGTTCATCTA    61 CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC   121 CATGGGCTGG GAGGCCTCCA CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA   181 GATCAAGATG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG TCAAGACCAC   241 CTACATGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AAGACCGACA TCAAGCTGGA   301 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG CCGAGGGCCG   361 CCACTCCACC GGCGCCATCG ATGAATTGAA TTCCATGCCG TCCGAGAAGA CCTTCAAACA   421 GCGCCGGAGC TTCGAACAAA GAGTGGAAGA TGTCCGGCTC ATCCGGGAGC AGCACCCCAC   481 CAAGATCCCA GTGATTATAG AGCGATACAA GGGTGAGAAG CAGCTGCCCG TCCTGGACAA   541 GACCAAGTTC CTTGTACCTG ATCACGTGAA TATGAGCGAA CTCATCAAGA TAATTAGAAG   601 GCGCCTGCAG CTCAATGCTA ACCAAGCCTT CTTCCTCCTG GTGAATGGGC ACAGCATGGT   661 GAGTGTGTCC ACACCCATCT CTGAAGTGTA CGAGAGCGAG AGAGATGAAG ACGGCTTCCT   721 GTACATGGTC TATGCCTCCC AGGANACGTT CTAANGATCC CAGTGTGGTG GTACGGGAAT   781 TCCTGCANGC CTCGAGGGCC GGCGCGCCGC GGCCGCTACG TAAATTCCGC CCCCCCCCCC   841 CCCCTCTNCN NNCCCCCCCC CNNNNCGTTN NNTGNNNNAN NNNGCTTNNN NNAAGGNCGG   901 GNGTGNGTTT GNCTATATGN TNATTTNNNN NNNTATTNNC NNNCNTTTTG NCANNGNGNN   961 NNNNNNNGNA ANCNNGNNCC TGNNTNNNTN GNNNANNANT NNNNGGGGNN NTTTCCCNNN 1021 NCNNNNNNNN GNANNGNANN NNNNNNNTNN NNNNNNNN //
Primer: EGFP-N (Pr0027) Region: pol,gag Sequence file: RDB14600_A7Bak.seq
>D04280A1_A7Ba_1_EGFP-N_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTGCTNNNNN TGGTGGCGGC GGGNGANCCG    61 GCAGTCTAGA GGATGGTCCA CCCCCGGGGT CGGCAGCCTT CACGTGGGCG GCGTGTATCC   121 AAGCTGCGAT GCCGTCTACT TTGAGGGCGG TGGGGGTGGT CAGCAGGACT GTGTAAGGTC   181 CTTTCCAGCG AGGTTCTAGG TTCTTAGTCT GGTGTCGGCG GACCCACACT GTGTCGCCGA   241 CTCGGTAAGG GTGAGGTACC ACCGGTCGGT CCAGTTGTTC TTGGTAGGCT GCCGCCAGAG   301 GTCTCCAGAC TTCGTGCTGG ACTAAGTAGA GAGCCTGTAA GTGAGCTTGG AGAGAGGGGC   361 TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC AAGGGGCGGG GGTGCCCCAT   421 ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG AGTGAGGGCT GGATAAAGGG   481 AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG AGACGGGGCG GATGGAGGAA   541 GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA GGGAGGGGGG TCAAAAGCCA   601 AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG TCCATGCGGG CCAGGTGAAA   661 AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA GGTGCCGTCT CGCGGCCATC   721 CGACGTTAAA GGTTGGNCAT TCTGCANAGC ANAANGNAAC CCAACGTCTC TTCTTGACNT   781 CTACCGACTG GTTGTGAGCG ATCCGCTCNA CATCTTTCCN GNGANCTAAN GNCAAACTTA   841 AGGGANTGNN AACNGTCTGN NCCNGGCCCA TATTTTNNNA NAANTACAGA AANNNNGNCN   901 GANNNAGACA NNNCNGAANN ATGCTGNNNC ANANAAGANN NNCGGNNCGN NTTCNGNNCN   961 NANNNNAAAG CNAAAANNTC NNANNNANNN NGGANNNGNT TNNNGNTCTN NNNNNCNNNC 1021 NNNANNNNNN NNNN //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid(EGFP 5'),pol Sequence file: RDB14600_A7Bal.seq
>14600_14600_B2FhF_EGFP-N_R2_A06_03_ABI24.ab1     1 ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NCNCCNNNGN CANGGNGGTC    61 ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC   121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG   181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC   241 ACCATGGTGG CGGCGGGAGA TCCGGCAGTC TAGAGGATGG TCCACCCCCG GGGTCGGCAG   301 CCTTCACGTG GGCGGCGTGT ATCCAAGCTG CGATGCCGTC TACTTTGAGG GCGGTGGGGG   361 TGGTCAGCAG GACTGTGTAA GGTCCTTTCC AGCGAGGTTC TAGGTTCTTA GTCTGGTGTC   421 GGCGGACCCA CACTGTGTCG CCNACTCGGT AAGGGTGAGG TACCACCGGT CGGTCCAGTT   481 GTTCTTGGTA GGCTGCCGCC AGAGGTCTCC AGACTTCGTG CTGGACTAAG TAGAGAGCCT   541 GTAAGTGAGC TTGGAGAGAG GGGCTGTTAG TAACTCTTGT CATGTCAGGG TCAGGGAAGT   601 TTACAAGGGG CGGGGGTGCC CCATATAAGA TCTCATATGG NCNNNTGGGG GCGCCTAGAG   661 AAGGAGTGAG GGCTGGATAA ANNGGAGGAT CGAGGCGGGG TCGAACGAGG NNGTTCAAGG   721 GGGANAGACG GNNNNNATGG AGGAANNANG AGGCGGANGC TTAGGGTGNN NNNNNGGGCT   781 TGACCCCANG GAGGGGGGNC AAAAGCNNAN NGCTTCCNNN NTCACNATGT NNGGGNNNNN 841 NNNNN //
Primer: mCherry-C_R (Pr0517) Region: insert mid(mRFP1 5',rat LC3 3') Sequence file: RDB14600_A7Bam.seq
>14600_14600_B2FhF_mCherry-C_R_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNCNGCN NNNNGTCGGT CTTGTAGGCG CCGGGCAGCT GCACGGGCTT    61 CTTGGCCNTG TANGNGGTCT TGACCTCGGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG   121 CCTCATCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGTGGAGGC   181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG   241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC   301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG   361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACTGGAA   421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCG CCCTTGGTCA CCTTCAGCTT   481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG   541 GCCGTTCACG GAGCCCTCCA TGCGCACCTT GAAGCGCATG AACTCCTTGA TGACGTCCTC   601 GGAGGAGGCC ATCCCGAACG TCTCCTGGGA GGCATAGACC ATGTACAGGA AGCCGTCTTC   661 ATCTCTCTCG CTCTCGTACA CTTCAGAGAT GGGTGTGGAC ACACTCACCA TGCTGTGCCC   721 ATTCACCAGG AGGAAGAAGG NTTGGTTAGC ATTGAGCTGC AGGCGCCTTC TAATTATCTT   781 GATGAGTTCG CTCATATTCA CGTGATCAGG TACAAGGAAC TTGGTCTTGT CCAGGANGGG   841 CAGCTGCTTC TCACCCTTGT ATCGCTCTAT AATCACTGGG ATCTTGGTGG GGTGCTGCTC   901 CCGGATGANC CGGANATCTN NCACTCTTTG TNCNAAGCTC CNGNGCTGTT TGNANGNCTT   961 CTCNNANNGN NNNGNNATTN NANTCNTCNN TAGATNCGNN GNNNCCNNAN NNNNNCCGNC 1021 CNNNNNNNNG NNNCCCNTNN NNNNNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: PuroR,IRES Sequence file: RDB14600_A7Ban.seq
>D04280A1_A7Ba_1_pMXs-3LTRup_R2_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT TNNTCGTCGA GCCNTGGGGT CGTGCGCTCC TTTCGGTCGG    61 GCGCTGCGGG TCGTGGGGCG GGCGTCAGGC ACCGGGCTTG CGGGTCATGC ACCAGGTGCG   121 CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG CCGAGCCGCT CGTAGAAGGG   181 GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC CCGGCGCGCT CGGCCGCCTC   241 CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC TGGTGGTCGG GCGAGACGCC   301 GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC GGCGCCAGGA GGCCTTCCAT   361 CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC ATGCGCGGGC CGATCTCGGC   421 GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG ACCGCCACCG CGGCGCCGTC   481 GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC GTGAGGAAGA GTTCTTGCAG   541 CTCGGTGACC CGCTCGATGT GGCGGTCCGG GTCGACGGTG TGGCGCGTGG CGGGGTAGTC   601 GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCGGGGGACG TCGTCGCGGG TGGCGAGGCG   661 CACCGTGGGC TTGTACTCGG TCATGGTTGT GGCCATATTA TCATCGTGTT TTTCAAAGGA   721 AAACCACGTC NNCGTGGTTC GGGGGGCCTA GACGTTTTTT TAACCTCGAC TAAACACATG   781 TAAAGCATGT GCACCGAGGC CCCAGATCAN ATCCCNTACN ATGGGGTACC TTCTGNNCAT   841 CCTTCNGCCC CTTGNTGAAT ACNCTNGANG ANAGNCNTTT GANTCNTTNC NCNNCTATCC   901 NACTCNCANC GNGGNACNNG GGNNGNGNCG CNTTNNCNNN NGNNNCTTAT ACNCNNGNNT 961 TTTNGNNNNC NNANNNNNCN GNNGNNNNGN NGGGGGGNNN NNNNNNN //
Primer: p15Aori_F (Pr0276) Region: ori,LTR Sequence file: RDB14600_A7Bao.seq
>D04280A1_A7Ba_1_p15Aori_F_F04_16_ABI24.ab1     1 NNNNNNNNNN NNNNTTTGNG NNGNTCGTCN GGGGGGCGGA NCCTATGGAA AAACGCCAGC    61 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT   121 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT   181 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA   241 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAACTA TGGCCATTTA   301 ATGTAAATAC TTAAGAAAAA AAACCAAATT AATTTTGATA CATGCTGCAT GTGAAAGACC   361 CCCGCTGACG GGTAGTCAAT CACTCAGAGG AGACCCTCCC AAGGAACAGC GAGACCACAA   421 GTCGGATGCA ACTGCAAGAG GGTTTATTGG ATACACGGGT ACCCGGGCGA CTCAGTCAAT   481 CGGAGGACTG GCGCCCCGAG TGAGGGGTTG TGGGCTCTTT TATTGAGCTC GGGGAGCAGA   541 AGCGCGCGAA CAGAAGCGAG AAGCGAACTG ATTGGTTAGT TCAAATAAGG CACAGGGTCA   601 TTTCAGGTCC TTGGGGCACC CTGGAAACAT CTGATGGTTC TCTAGAAACT GCTGANGGCT   661 GGACCGCATC TGGGGANCAT CTGTTCTTGN NCCTGANCCG GGGCAGGAAC TGCTTANCAC   721 AGATATCCTG TTTGGNCCAT ATTCNGCTGT TCCATCTGTT CTTGGNCCTG ANCCGGGGCA   781 NGANCTGCTT ACCNCAGATA TCCTGNTTGG NCCATATTCN GCTGTNCCNT CTGNTCCTGA   841 NCTTGATCTG AACTTCTCTA TTCTCAGTTA TGTATTTTTC CNTGCCTNGN AAAATGGCGT   901 TACTTAANCT AGNTTGNCNA NNNTACNNNN GGGGNNNTNC NTTCCNNCCT TTTTCNGNAN   961 ANTAAANAAA ATCNTTTANT TNATCNNCNA NNNNNGGGGN CNNGNNNNNN NTTNNNNNNG 1021 NNNNNNNNGN NNNNNNNNN //
Primer: MMLV psi_R1 (Pr1054) Region: MMLV psi,CMV en Sequence file: RDB14600_A7Bap.seq
>14600_14600_B2FhF_MMLV psi_R1_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNANCNN GNNNGNNNCN GATAAGTTGC TGGCCNGCTT ACCTCCCGGT    61 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCCGATCCCG GACGAGCCCC CAAATGAAAG   121 ACCCCCGCTG ACGGGTAGTC AATCACTCAG AGGAGACCCT CCCAAGGAAC AGCGAGACCA   181 CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG CGACTCAGTC   241 AATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC   301 AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG   361 TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGCA TGGTAATAGC   421 GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC   481 ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG   541 ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA   601 ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG   661 GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC   721 CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAGTCAATAA   781 TCAATGTCAA CATGGCGGTA ATGTTGGACA TGANCCAATA TAAATGTACA TATTATGATA   841 TGGATACNAC GTATGCAATG GGCCNAGCTC CTCGAGTGAA GANGAAANGG CCTCGNGATA   901 CGCCNATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTANACGTC NNGNGGNNNT   961 TTTCNGGGAA ATGTGCNNNG ANNCCCNANT TGNTNATTTT NCTAANNACN TNNNANNNNG 1021 TANNCNNCNN NNNANNNNNN NNNCNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.04

HTML_filter_RDB_240329.pl