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pcDNA4/chicken_ST3Gal5-His

Catalog number RDB14638
Resource name pcDNA4/chicken_ST3Gal5-His
Clone info. Expression vector of chicken beta-galactoside alpha-2,3-sialyltransferase 5 (ST3Gal5), C-terminal myc- and 6xHis-tag. cDNA fragment 128nt to 1231nt of NM_001001192.1 is cloned.
Comment Primers were designed following the putative DNA sequence data of chicken ST3Gal5, NM_001001192. Expression was confirmed by the depositor with Western blotting and RT-qPCR. Known difference: A to G substitution at 433nt (Lys102, synonym).
Vector backbone pcDNA4/TO/Myc-HisA (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells)
Gene/insert name Gallus gallus ST3GAL5 cDNA
Depositor|Developer Kaneoka, Hidenori |
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External Database
Gallus gallus ST3GAL5

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Kidani, S et al. J Biosci Bioeng. 122 (4) : 379-384, 2016) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 1.利用者は、研究成果の公表にあたって開発者の指定する文献を引用する(Kidani, S et al. J Biosci Bioeng. 122 (4) : 379-384, 2016)。2.利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。3.営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          RDB14638 pcDNA4/chicken_ST3Gal5-His Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Kidani, S., Analyses of chicken sialyltransferases related to O-glycosylation. J. Biosci. Bioeng. 122 (4): 379-384 (2016). PMID 27150510.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Kidani, S., Analyses of chicken sialyltransferases related to O-glycosylation. J. Biosci. Bioeng. 122 (4): 379-384 (2016). PMID 27150510.

          2022.05.17

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