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pEThT-MAHS delta MTP (#RDB14653)

Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Mitochondrial Abundant Heat Soluble (MAHS) without mitochondia targeting peptide in E. coli.

Clone info. pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Mitochondrial Abundant Heat Soluble (MAHS) without mitochondia targeting peptide under the T7 promoter for expression recombinant protein in E. coli.
Comment Expression was confirmed by the depositor with Heat-solubility assay.
Vector backbone pEThT (plasmid)
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus MAHS cDNA
Reference of insert sequence LC002822 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14653 pEThT-MAHS delta MTP Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pEThT-MAHS delta MTP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14653).

Reference section:

Tanaka, S., Tanaka, J., Miwa, Y., Horikawa, D.D., Katayama, T., Arakawa, K., Toyoda, A., Kubo, T., Kunieda, T., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tanaka, S., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104. [link to RRC of NBRP]