Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-Rab18

Expression vector of human Rab18, wild type, fused with mCherry.

Catalog number RDB14699
Resource name pDEST/mCherry-Rab18
Clone info. Expression vector of human Rab18, wild type, fused with mCherry.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
Other clones in our bank

External Database
human RAB18

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14699 pDEST/mCherry-Rab18 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14699_A7Djp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mCherry-C_F
          Sequence file: RDB14699_A7Dja.seq check
          >D04483A3_A7Dj_1_mCherry-C_F_A05_02_ABI24.ab1
              1 TAACAAGCAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
             61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
            121 ACCGGCGGCA TGGACGAGCT GTACAAGAAG CTTACAAGTT TGTACAAAAA AGCAGGCTCC
            181 GCGGCCGCCT TGTTTAACTT TAAGAAGGAG CCCTTCACCA TGGACGAGGA CGTGCTAACC
            241 ACCCTGAAGA TCCTCATCAT CGGCGAGAGT GGGGTGGGCA AGTCCAGCCT GCTCTTGAGG
            301 TTCACAGATG ATACGTTTGA TCCAGAACTT GCAGCAACAA TAGGTGTTGA CTTTAAGGTG
            361 AAAACAATTT CAGTGGATGG AAATAAGGCT AAACTTGCAA TATGGGATAC TGCTGGTCAA
            421 GAGAGGTTTA GAACATTAAC TCCCAGCTAT TATAGAGGTG CACAGGGTGT TATATTAGTT
            481 TATGATGTCA CAAGAAGAGA TACATTTGTT AAACTGGATA ATTGGTTAAA TGAATTGGAA
            541 ACATACTGTA CAAGAAATGA CATAGTAAAC ATGCTAGTTG GAAATAAAAT CGATAAGGAA
            601 AATCGTGAAG TCGATAGAAA TGAAGGCCTG AAATTTGCAC GAAAGCATTC CATGTTATTT
            661 ATAGAGGCAA GTGCAAAAAC CTGTGATGGT GTACAATGTG CCTTTGAAGA ACTTGTTGAA
            721 AAGATCATTC AGACCCCTGG ACTGTGGGAA AGTGAGAACC AGAATAAAGG AGTCAAACTG
            781 TCACACAGGG AAGAAGGCCA AGGAGGAGGA GCCTGTGGTG GTTATTGCTC TGTGTTATAA
            841 AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TTGATCTAGA GGGCCCGCGG
            901 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
            961 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
           1021 CAGCATCTGT GTTGCCCCTC CCCCGTGCCT TCATGACCTG AGTGCCACTC CCACTGTCGT
           1081 TCTTATTAAT GAAGAAATGC ATCGCATTGT TGATAGTTCA TTCTATCTGG GGGGTTGGGA
          1141 TTGAGCCAGG AA
          //
          Primer: BGH_rev2
          Sequence file: RDB14699_A7Djb.seq check
          >D04483A3_A7Dj_1_BGH_rev2_B05_05_ABI24.ab1
              1 GTATTCCATA AGCAAGTAAC ACGTCACACA GGGCAGCAGC AGATGGCGTG GCAACTAGAA
             61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACTCAATGGT GATGGTGATG ATGACCGGTA
            121 CGCGTAGAAT CGAGACCGAG GAGAGGGTTA GGGATAGGCT TACCTTCGAA CCGCGGGCCC
            181 TCTAGATCAA CCACTTTGTA CAAGAAAGCT GGGTCGGCGC GCCCACCCTT TTATAACACA
            241 GAGCAATAAC CACCACAGGC TCCTCCTCCT TGGCCTTCTT CCCTGTGTGA CAGTTTGACT
            301 CCTTTATTCT GGTTCTCACT TTCCCACAGT CCAGGGGTCT GAATGATCTT TTCAACAAGT
            361 TCTTCAAAGG CACATTGTAC ACCATCACAG GTTTTTGCAC TTGCCTCTAT AAATAACATG
            421 GAATGCTTTC GTGCAAATTT CAGGCCTTCA TTTCTATCGA CTTCACGATT TTCCTTATCG
            481 ATTTTATTTC CAACTAGCAT GTTTACTATG TCATTTCTTG TACAGTATGT TTCCAATTCA
            541 TTTAACCAAT TATCCAGTTT AACAAATGTA TCTCTTCTTG TGACATCATA AACTAATATA
            601 ACACCCTGTG CACCTCTATA ATAGCTGGGA GTTAATGTTC TAAACCTCTC TTGACCAGCA
            661 GTATCCCATA TTGCAAGTTT AGCCTTATTT CCATCCACTG AAATTGTTTT CACCTTAAAG
            721 TCAACACCTA TTGTTGCTGC AAGTTCTGGA TCAAACGTAT CATCTGTGAA CCTCAAGAGC
            781 AGGCTGGACT TGCCCACCCC ACTCTCGCCG ATGATGAGGA TCTTCAGGGT GGTTAGCACG
            841 TCCTCGTCCA TGGTGAAGGG CTCCTTCTTA AAGTTAAACA AGGCGGCCGC GGAGCCTGCT
            901 TTTTTGTACA AACTTGTAAG CTTCTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG
            961 CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC
           1021 CACTTGATGT TGACGTTGTA GCGCCGGCAG CTGCACGGGC TCTGGCTTGT AGTGTCTGGA
           1081 CTCAGCGTCG TATGATGCGG TCTTCAGCTT CAGCTCTGCT TGGATCTCGC CCATCAGGAC
          1141 GCCGATCCCT CGGGGGTAC
          //
          Primer: CMV-Forward
          Sequence file: RDB14699_A7Djc.seq check
          >D04483A3_A7Dj_1_CMV-Forward_C05_08_ABI24.ab1
              1 ACTAATCAAC TTTCCGCCCC AATTGACGCA AATGGGCGGT AGGGCGTGTA CGGTGGGAGG
             61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
            121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTATCAG GTACCATGGT
            181 GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA
            241 CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC
            301 CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC
            361 CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC
            421 CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT
            481 GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA
            541 GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA
            601 GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT
            661 GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT
            721 CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT
            781 CAAGTTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC
            841 CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGAAGCTTA CAAGTTTGTA
            901 CAAAAAAGCA GGCTCCGCGG CCGCCTTGTT TAACTTTAAG AAGGAGCCCT TCACCATGGA
            961 CGAGGACGTG CTAACCACCC TGAAGATCCT CATCATCGGC GAGAGTGGGG TGGGGCAAGT
           1021 CCAGCCCTGC TCTTGAGGTC ACAGATGATA CGGTTTGATC CAGACTGCAG CACAATAGGT
           1081 GTGACTTAGG TGAAACAATT TTCATGATGA ATTAGGCCTT AACTGCAATA TGGGATAACC
          1141 TGCCTGGGTC AAGAAGAGGA GAAGT
          //
          Primer: SV40pro_F
          Sequence file: RDB14699_A7Djd.seq check
          >D04483A3_A7Dj_1_SV40pro_F_A01_01_ABI24.ab1
              1 CCCCGTCCTT ACTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
            961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCCGC CCTTCTATGA AAGGTTGGGC
           1021 TTTCGGGAAT CGTTTTCCGG GGACGCCCGG GCTGGGATGA TCCTCCAGCG CGGGGATCTC
           1081 ATGCTGCAGT CTTCGCCCAC CCCCAAACTG GTTATGGCCA GCCTATATTG TTACAATTAA
          1141 GCAGTAGCCA TCAACAAATT TTCGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl