Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-mouse TBC1D2B

Expression vector of mouse TBC1 domain family member 2B (TBC1D2B), fused with N-terminal EGFP, CMV promoter.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14984_A7Egp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert 5' Sequence file: RDB14984_A7Ega.seq
>14984_14984_B2JqF_2_mVenus-M_F_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC NNCNAGGTGA NTTCNAGNNC CGCCNCAACA TCGAGGANGG    61 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT   121 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA   181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA   241 CGAGCTGTAC AAGTCCGGAC TCAGATCTAT GCCGGGGGCC GGGGACGGCG TGGAGGAGAG   301 CTGCAGTGGT GGTGAGGGAG CGGTACCGGG AACAGGCTCG GAGGCTGGGG CGGTGGCGGG   361 ACGGGAGCCC TCGCGGCTGT GCGGCTACCT GCAGAAGCTG TCGGGCAAGG GCCCGTTGCG   421 CGGCTACCGC AGCCGCTGGT TCGTGTTTGA CTCGCGCCGC TGCTACCTCT ACTACTTCAA   481 GAGTCCCCAG GACGCTCTGC CGCTCGGCCA CCTGGACATC GCCGACGCCT GCTTCAGCTA   541 CCAGGGCCGC GATGAGGCTG CGGAGCCCGG AGCAGATCCG CCCACACACT TCCAGGTGCA   601 CAGCGCCGGT GCAGTCACCG TGCTCAAGGC TCCTAATCGT GAGCTCATGA CTTACTGGCT   661 ACAGGAGCTT CAGCAGAAAA GATGGGAATA TTGCAACAGC CTTGACATGA TGAAGTGGGA   721 CAGCANGACT TCCCCGACTC CNGGGGANTT TCCCAAGGGC CTTGTGGCCN GAGATACCAC   781 AGATATAATT TCCCAGCACC CAAATCCTTC TGCAGAAAAN GNCAGNNNTG TGCNCGCTGN   841 GGAAGCTGCC CCTGGAGAGC TGGNGGGAGA NNGGGCTGCT CATCAGCCNG CTCCCGGGNA   901 TNNNAANNNC CNTCNACTTT TTACTCTCTG ANNNNNNGGG GNAATGANCT CNAAAATTNN 961 NNNNCNTCNT TNNNNNNNNG GGNNNNGNNN NNNNNNN //
Primer: SV40polyA-R (Pr0109) Region: insert 3' Sequence file: RDB14984_A7Egb.seq
>D04556B5_A7Eg_2_SV40polyA-R_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTNANACC TCTACNAATG TGGTATGGCT GATTATGATC    61 AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACCTGCAG GCGGCCGCGA   121 ATTCACTAGT GATTTCAAGT GTCTTCCTCC TCATCGCTAA CTAGCTCGCC TTTGTCAGGG   181 CTAGTGTCCC GCTCACGCAG GAAGTCCTCT CGAATGGCCT CCAGCTCTGT CAGCTCCAGC   241 CGGACCTTCT CCAGGTGGTA GGCTCTCCGG TTCCGGATCT GGCGCAGGGG GAAGGGGTTC   301 AGATCCCCAA AGGAGATGCT GATCAGCTTC CTGGCATCGA GGATAGTCCG AGTGAAGTAA   361 CGCAGATACT TGAATATGGA CATCGAATCT TGCAATTTCA GGATCTCCTC TTCCTTATAT   421 TTAAAAAGTG CCAGGGCAAA ACGGAAAATA ACCTTTGGTC CCTCATAAAG GAAAGAGTCC   481 CATATTTTAA AGAGGATGTC GCTGACAACG CTGTCCACAA ATACCACCAG AAACCAGTTG   541 AAGGTGATGA GGGTGTAGTC CACTTTGTAC TGTTCAAAAT GGGTGTGCAG TCGAGGCAGC   601 TTCTCACTCA GGAGGTCTCT GAACACCCGC TGGTCCACCT GGGATCCTAA AAGAGTCTTT   661 GTGTAATAGT CTCGGGGCAT GAAGACTTCC ACGATGGTAA CGAGACACCA GAAGGCATCT   721 TCTTGATCCA GGTAAAGGAG AGCCACTGCC ACCAACCTGT TCAGGCCTTG GCAGTAGCCG   781 ATATCGGGAT TCCGCCAGGA NAANGCGAGC ANGACACTGC GCAGCTTCTG TATGCCCTCC   841 GACGTGGGGC TGGAGTANTG NTTGTTGTTG GGCAGANTCC GGANCANGNC AAGCTCGATC   901 TGCTNNNANG NCGGGTTCTG TTTNTNNNGA NCCTTCTGCA GCAAGGNCTG GNANTAGTCT   961 GGCNNCNTGC TGNNNNTGNN NNTNCNNNNN NNGANGGNCN NCNCNNNNNN TNNNNNNNNN 1021 N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB14984_A7Egc.seq
>14984_14984_B2JqF_2_CMV_Forward_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ANNNNNNNNN NNNNNNGNNN NNNNNNNNNN    61 NNNNTNNNNN NGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC   121 ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG   181 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC   241 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC   301 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG   361 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC   421 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC   481 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG   541 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG   601 AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC   661 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC   721 CACTACCTGA GCACNNNNNC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG   781 GTCCTGCTGG AGTTCGTGAC CGCCGCNGGG ATCACTCTCG GCATGGACNA NCTGTACNAG   841 TCCNGACTCA NNNCTATGCC NGGGGGCCNG GGANGGCGTG NANGANANCT GCAGTGNTGG   901 TGANGGANCG GTACCNGGAA CNGGCTCNNN NNNNGGGGNN GNNNGNNGGG ANNGGNNNCN   961 NCGNNGNNGT GNNGGCNNCC NNCANANNCN NNNNGGNNAA NGGNCNNNNT NNNNNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB14984_A7Egd.seq
>D04556B5_A7Eg_2_SV40pro_F_V2_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNN NGCTTTTGCA AGNNCGATCN AGAGANAGGA TGAGGANCGT    61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC   601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC   721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC   781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA NCGCATCGCC TTCTATCGCC   841 TTCTTGACGA NTTCTTCTGA NCGGGANTCT GGGGGTTCGA AANGACCGAN CNAGCGACGC   901 CNNACCTGCC NTCNCGAGAT TTCGANNCCA CCGCCNCCTT CTATGAAAGG NTGGNNTNCG 961 GNNNNNTTTN CCNGGNNGNN NGNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl