RDB14994 - pEGFP-C1-human TBC1D10A

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pEGFP-C1-human TBC1D10A

Expression vector of human TBC1 domain family member 10A (TBC1D10A), fused with N-terminal EGFP, CMV promoter.

Catalog number	RDB14994
Resource name	pEGFP-C1-human TBC1D10A
Alternative name	EPI64
Clone info.	Expression vector of human TBC1 domain family member 10A (TBC1D10A), fused with N-terminal EGFP, CMV promoter.
Comment	Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	human TBC1D10A cDNA
Reference of insert sequence	AB449894 (DDBJ accession number)
Depositor\|Developer	Fukuda, Mitsunori \|

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human TBC1D10A

External Database
human TBC1D10A

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14994	pEGFP-C1-human TBC1D10A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14994_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB14994_A7Ega.seq
>D04556C6_A7Eg_2_EGFP-C2_H04_22_ABI24.ab1 1 GCCCCACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCGAAGA GCAACGGAGA 121 GAATGGGCCG CGCGCGCCCG CGGCCGGGGA AAGCCTGTCG GGAACCCGGG AGAGCCTGGC 181 CCAGGGCCCC GACGCCGCAA CCACCGACGA ACTCAGCTCT CTCGGGTCTG ACTCGGAGGC 241 CAACGGCTTC GCCGAGCGCC GCATCGACAA GTTCGGCTTC ATCGTGGGCT CGCAGGGCGC 301 CGAGGGCGCG CTGGAGGAAG TACCCCTGGA GGTGCTGAGG CAGAGGGAGT CCAAGTGGCT 361 GGACATGCTC AACAACTGGG ACAAATGGAT GGCCAAGAAG CACAAAAAGA TTCGTCTGCG 421 GTGCCAAAAG GGCATCCCGC CTTCTCTGCG GGGCCGTGCT TGGCAGTACC TGTCAGGAGG 481 CAAGGTGAAG TTACAGCAGA ACCCTGGAAA GTTTGACGAG CTGGACATGT CCCCTGGGGA 541 CCCCAAGTGG CTGGACGTGA TTGAGCGTGA CCTGCACCGG CAGTTCCCAT TCCATGAGAT 601 GTTTGTGTCC CGGGGGGGCC ACGGCCAGCA GGACCTATTC CGTGTGCTGA AGGCCTACAC 661 GCTGTACCGG CCCGAGGAGG GCTACTGCCA GGCCCAGGCG CCCATTGCCG CTGTCTTGCT 721 CATGCATATG CCTGCTGAGC AAGCCTTCTG GTGCCTGGTA CAGATCTGTG AGAAGTACCT 781 GCCCGGCTAC TACAGCGAGA AACTGGAGGC GATCCAGCTG GACGGGGAGA TCCTTTTCTC 841 GCTGTTGCAG AAAGTGTCGC CGGTGGCCCA CAAGCACCTC AGCCGTCAGA AGATCGACCC 901 GCTCCTTTAT ATGACAGAAT GGTTCATGTG CGCCTTCTCC CGAACCTTGC CCTGCAGCTC 961 TGTGCTGCGT GTCTGGGACA TGTTCTTCTG TGAAAGGGGT CAAGATCATC TTCCGGGTGG 1021 GGCTGGTGCT GCTGAAGCAC CGCCGCTGGC TCCCCCTGAG AGTCAAAGGC TTGCAGGCCA 1081 GTACGAGACA ATCGAGCGAC TGCCGAGACT CAGCCTCAGA TTCATGCCAT GAGTGCTTCA 1141 TGTCAAGGAA CG //
Primer: SV40polyA-R Sequence file: RDB14994_A7Egb.seq
>D04556C6_A7Eg_2_SV40polyA-R_H05_23_ABI24.ab1 1 AAAAATTGGG AAGTTTTTTA AGCAGTAAAA CCTCTACAAA TGTGGTATGG CTGATTATGA 61 TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC AGGCGGCCGC 121 GAATTCACTA GTGATTGGTT ACAAGTAGGT GTCCTCACTC TCTTGGGACG TCAAGCTCTC 181 CTGGGAGCGG TGGTGGGCTG AGACCTGGGG AGCCAAATCC TGAGGGGCTG AGTCCTTGGG 241 GGCTGAGTCC TTCGGGGGCA CATGCTGTGG GGGACATGCA TCTCCTGCAG CGGCCACCAC 301 CATGGCTTGA TTTGGGGCTG GGGGCTTCTC CAGCTGCCCT CTCCCCTTCA TCTGTTTCCG 361 CTGCTCCTTC TGGGCCTGCT TGGGTGGCTT GGGCTTGGCT TTGGAGCCAG GGAGGGGGGC 421 ATCTAGGGGC AGGCGGATGG ATGGTGAAGG TTGTAGGGCA GGCCGGGGAC CAGGTTCTGC 481 ATCCAAGATA GCCTTGGCAC CATGCAGCCT GGGCGGGGAG CGGCACTGCA GCTCACCCCG 541 GGTCTCCTGC CAGCGCCGCA GCTGAATGAG GTGTTCGCGC TCAATCTGGC GCTCTGTCAC 601 GGGCAACTCC ACCACCTCCT GGACCAGAAA GGCCTCCTGC ATGATCTTGG GGCTGAGGCT 661 CCGCAGTCGC TCGATGGTCT CGTACTGGCC CTGGCAGGCT TTGACCTTCT CAGGGGAGCC 721 CAGCGCGTGC TTCAGCAGCA CCAGCCCCAC CCGGAAGATG ATCTTGACCC CTTCACAGAA 781 GAACATGTCC CAGACACGCA GCACAGAGCT CCAGGGCAAG GTTCGGGAGA AGGCGCACAT 841 GAACCATTCT GTCATATAAA GGAGCGGGTC GATCTTCTGA CGGCTGAGGT GCTTGTGGGC 901 CACCGGCGAC ACCTTCTGCA ACAGCGAGAA AAGGATCTCC CCGTCCAGCT GGATCGCCCT 961 CCAGTTTCTC GCTGTAGTAG CCGGGCAGGT ACTTCTCACA GATCTGTACC AGGCACCAGA 1021 GGCTTGCTCA GCAGCATATG CATGAAGCAA GACAGCGGGC ATGGGGCGCC CTGGGTCTGG 1081 CAGGTAGTCC CTCCTTCGGA TCGGTACAGC GTGTAAGGCT TCCAGCCACA CGGAATAGGA 1141 TCCTTGGCCT GGGGACCGG //
Primer: SV40pro_F_V2 Sequence file: RDB14994_A7Egc.seq
>D04556C6_A7Eg_2_SV40pro_F_V2_H06_24_ABI24.ab1 1 GTCCAGCTTT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT 61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC 601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC 901 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA 961 ATCGTTTTCC CGGACGCCCG GCTGGATGAT CCCTCCAGCG CGGGGATCTC ATGCTGGAGT 1021 TCTTCGCCCA CCCTACGGGA GCTACTGAAA ACACGGGAAG AGACATACCG GCATGACCCT 1081 GCTATGACGG CATTAAAGAC TGATAAACGC CACCGGTGTT TGGGATC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
reference	Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2023.04.10

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