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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-human TBC1D28 (#RDB15015)

Expression vector of human TBC1 domain family member 28(TBC1D28), fused with N-terminal EGFP, CMV promoter.

Clone info. Expression vector of human TBC1 domain family member 28(TBC1D28), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D28 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15015 pEGFP-C1-human TBC1D28 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D28 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15015).

Reference section:

Oguchi, M.E., Noguchi, K., Fukuda, M., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15015_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 ()
Sequence file: RDB15015_A7Ega.seq check
>D04556E9_1_EGFP-C2_E02_14_ABI24.ab1
    1 GAACCACCGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGAGATGG ATGAGGACCC
  121 GGATAACCTG CCTGCCCAGG GGCAAGGCAA TATCATCATT ACTAAGTATG AGCAGGGACA
  181 CCGAGCTGGG GCAGCAGTGG ACTTGGGGCA TGAGCAGGTT GATGTCAGAA AATACACCAA
  241 TAACCTCGGG ATTGTGCATG AGATGGAGCT GCCCCGCGTC AGTGCCCTGG AGGTGAAGCA
  301 AAGACGCAAG GAAAGTAAAC GTACCAACAA GTGGCAAAAG ATGCTTGCAG ACTGGACAAA
  361 ATATAGGAGC ACCAAGAAGC TGTCTCAAAG AGTATGCAAA GTCATTCCCC TGGCGGTGCG
  421 GGGCCGGGCG TTGTCACTTT TGCTAGATAT TGACAAAATC AAGTCCCAGA ACCCAGGCAA
  481 ATATAAGGTC ATGAAGGAGA AGGGCAAGAG GTCCTCCAGA ATCATCCACT GCATCCAGCT
  541 AGATGTCAGC CACACCCTGC AGAAACACAT GATGTTCATA CAAAGATTCG GAGTCAAGCA
  601 GCAGGAATTA TGTGACATCC TCGTGGCCTA TTCTGCATAT AACCCTGTGA GTATTCCCGG
  661 GCAGCGATAT TCCTGGTACC TGTGCCCATA TTCACAGGCA TGGGTGTCTC TCGGGGGTGT
  721 TGCAACTTCT TGAATCGAAT TCCCGCGGCC GCCTGCAGGT CGACGGTACC GCGGGCCCGG
  781 GATCCACCGG ATCTAGATAA CTGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC
  841 TTGCTTTAAA AAAACCTCCC ACACCTCCCC CCTGAACCTG AAACATAAAA TGAATGCAAT
  901 TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
  961 AAATTTCACA AATAAAGCAT TTTTTTTTTC ACTGCATTTC TAGTTGTGGG TTTGTCCCAA
 1021 AACTCATCAA ATGTATCTTA ACGCGTAAGT GTTAAGCGAT ATATTTTTGA TAAAATTCGC
1081 GATTCAAGTT TGATAAATCA AGCCTTCAAA TTTTTTT
//
Primer: pAxEF_R2 ()
Sequence file: RDB15015_A7Egb.seq check
>D04556E9_1_pAxEF_R2_F02_17_ABI24.ab1
    1 CAAGGTCAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC
  241 GCGGGAATTC GATTCAAGAA GTTGCAACAC CCCCGAGAGA CACCCATGCC TGTGAATATG
  301 GGCACAGGTA CCAGGAATAT CGCTGCCCGG GAATACTCAC AGGGTTATAT GCAGAATAGG
  361 CCACGAGGAT GTCACATAAT TCCTGCTGCT TGACTCCGAA TCTTTGTATG AACATCATGT
  421 GTTTCTGCAG GGTGTGGCTG ACATCTAGCT GGATGCAGTG GATGATTCTG GAGGACCTCT
  481 TGCCCTTCTC CTTCATGACC TTATATTTGC CTGGGTTCTG GGACTTGATT TTGTCAATAT
  541 CTAGCAAAAG TGACAACGCC CGGCCCCGCA CCGCCAGGGG AATGACTTTG CATACTCTTT
  601 GAGACAGCTT CTTGGTGCTC CTATATTTTG TCCAGTCTGC AAGCATCTTT TGCCACTTGT
  661 TGGTACGTTT ACTTTCCTTG CGTCTTTGCT TCACCTCCAG GGCACTGACG CGGGGCAGCT
  721 CCATCTCATG CACAATCCCG AGGTTATTGG TGTATTTTCT GACATCAACC TGCTCATGCC
  781 CCAAGTCCAC TGCTGCCCCA GCTCGGTGTC CCTGCTCATA CTTAGTAATG ATGATATTGC
  841 CTTGCCCCTG GGCAGGCAGG TTATCCGGGT CCTCATCCAT CTCCATGGAT CTGAGTCCGG
  901 ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TTCCAGCAGG
  961 ACCATGTGAT CGCGCTTTCT CGTTGGGGGT CTTTTGCTCA GGCGGACTGG TGCTCAGTAG
 1021 TGAATTGGTC GGCAGCAGCA CGGGGCCGTC GCGAATGGGG GTGTTTCTGC TGGTATGGAT
1081 CGGCAGCTTG CACGCTGCGG TCTCGAATTG TTTTGGTGGC CC
//
Primer: CMV_Forward ()
Sequence file: RDB15015_A7Egc.seq check
>D04556E9_1_CMV Forward_G02_20_ABI24.ab1
    1 TTTTTCAAAT TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC
  841 TCAGATCCAT GGAGATGGAT GAGGACCCGG ATAACCTGCC TGCCCAGGGG CAAGGCAATA
  901 TCATCATTAC TAAGTATGAG CAGGGACACC GAGCTGGGGC AGCAGTGGAC TTGGGGGCAT
  961 GAGCAGGTTG ATGTCAGAAA ATACACCCAA TAACCCTCGG GATTGTGCAT GAGATGGGAG
 1021 CTGCCCCCGC GTCAGTGCCC TGCAGTGGAG GCAAGGACGC AAGAAAGTTA ACGTAACCCA
1081 ACAGTGCCAA GATCGCGTGC GACTGGACCA AATTAATAG
//
Primer: f1ori_R3 ()
Sequence file: RDB15015_A7Egd.seq check
>D04556E9_1_f1ori_R3_H02_23_ABI24.ab1
    1 GCCAAGGGGC ATTTTTTGGG GGAATGTGCG CGGACCCCTA TTTGTTTATT TTTCTAAATA
   61 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
  121 AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG
  181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
  241 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC
  301 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
  361 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
  421 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
  481 GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT
  541 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA
  601 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
  661 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG
  721 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT
  781 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT
  841 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG
  901 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
  961 ACTCCGGATG GAAGCCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGCT
 1021 CGCGCCAGCC CGAACCTGGT TCGCCAGGCT CAGCGAAGCA TGCCCGACCG CGAAGAATCT
 1081 CGTTCGTGAA CCCATGCGAT GCTTGCCATT GCCGGATATC ATGGTTGGAA AAATGGGCCG
1141 CCCAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oguchi, M.E., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]