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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A

Gateway(R) entry vector with human Period 3 [-3.1 to +0.2 kb]-luciferase fragment.

Catalog number RDB15084
Resource name human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A
Clone info. Human PER3 promoter sequence (KpnI-XhoI 3.3 kb fragment from BAC clone “RP3-467L1”) followed by firefly luciferase gene (pGL3, Promega) is inserted into pENTR-1A (Invitrogen). Cloned fragment: 7781586 to 7784879(NC_000001.11, except 7784236A/G, 7784252A/G); relatively -3117 to +177, where +1 corresponds to 1 nt of NM_016831.1.
Vector backbone pENTR-1A (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Kan^r
Gene/insert name human PER3 genomic Firefly luciferase cDNA
Depositor|Developer Akashi, Makoto |
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External Database
human PER3 Firefly luciferase

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamaguchi, A et al. Sci. Rep. 7(1): 6824, 2017)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, A et al. Sci. Rep. 7(1): 6824, 2017)。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15084 human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15084).

          Reference section:

          Yamaguchi, A., Matsumura, R., Matsuzaki, T., Nakamura, W., Node, K., Akashi, M., A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans. Sci Rep. 7 (1): 6824 (2017). PMID 28755004.
          Matsumura, R., Akashi, M., Multiple circadian transcriptional elements cooperatively regulate cell-autonomous transcriptional oscillation of Period3, a mammalian clock gene. J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15084_A8Ctp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: rrnBT1ter_F (Pr0488)
          Region: rrnB T2 ter,attL1,insert 5'(hPer3 pro 5')
          Sequence file: RDB15084_A8Cta.seq check
          >D04733A2_A8Ct_2_rrnBT1ter_F_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNGTNNT NNNNNNNGCC CGGANGGTGG CGGGCAGGAN GCCCGCCATA
             61 AACTGCCAGG CATCAAACTA AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT
            121 ACAAACTCTT CCTGTTAGTT AGTTACTTAA GCTCGGGCCC CAAATAATGA TTTTATTTTG
            181 ACTGATAGTG ACCTGTTCGT TGCAACAAAT TGATAAGCAA TGCTTTTTTA TAATGCCAAC
            241 TTTGTACAAA AAAGCAGGCT TTAAAGGAAC CAATTCAGTC GACTGGATCC GGTACCCAGC
            301 ATTTCCGCAT ACCCCAGTGA ACAGAAGAAA ACCGCTCCCC TTGTGAAAAG TGCTTTTTAG
            361 TATAGGCGAT GAGGAGGAAA CTGGCTTTTA CACAATTACC AAAATTCAGG ATGTGGCTTT
            421 GGAGTTCCCT AAATAGATGG TCTTTCTAAA GTGATGTGCA GAGCCGACCC CGTCTGTGGT
            481 AGACTAGATG GCTGCTGGCA TGGACCCTGG GATAGATGAG TGGATAGGTG AGGGACATCC
            541 CCAGACACAT GGCTCTGTTC CTGTAGATCC TTTTTTCCTC ATGAATGTAT TTCCATTCTT
            601 ATCGTAGAAC CCCTAGGAAA CAATATAATA CGGGTGGAAG AGGACTTTTG CTTATGTTTA
            661 CAATAGGAAA ATCCAAGTCC CTTTCCCGAA TTACAGGTCC CACGATGATC TGGTCCTTTC
            721 TAGTTTTCTG ACTTCATGTC ACACGACTCT CCAGCTCTCT CTTCTCCAAG CAAGGTACTC
            781 CACCAAAGTA TAGCTGTTGA CATCGGAGAG ACTTGTGCCC ATGAGACTTT CNACATCAGG
            841 GTGTGGCTTG AANGCCAGAN TTAGCTATGA ATGAAAACAG GCAANGAAGC CCTGANGGAN
            901 AAATNNANCT GATAAAGTTT GNTTCNGTTT CTCATCAGCA AANTGACTTC TTTNCNANNN
          961 NCTNNNGNNN NNANNTNCAG NNNNNNCNNG NNNNNNNNNN
          //
          Primer: KanR-N_R (Pr0530)
          Region: KanR,attL2,insert 3'(SV40 pA,luc 3')
          Sequence file: RDB15084_A8Ctb.seq check
          >D04733A2_A8Ct_2_KanR-N_R_C05_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNNN ATAAATCNGC ATCNNTGTTG GAATTTAATC GCGGCCTCGA
             61 CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG
            121 TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA
            181 CACGGGCCAG AGCTGCAGCT GGATGGCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT
            241 GTTCGTTGCA ACAAATTGAT AAGCAATGCT TTCTTATAAT GCCAACTTTG TACAAGAAAG
            301 CTGGGTCTAG ATATCTCGAC GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT
            361 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
            421 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
            481 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
            541 TGTATCTTAT CATGTCTGCT CGAAGCGGCC GGCCGCCCCG ACTCTAGAAT TACACGGCGA
            601 TCTTTCCGCC CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC
            661 CGGTAAGACC TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG
            721 TTGTTACTTG ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT
            781 CCAAAACAAC AACGGCGGCG GGAAGTTCAN CGGCGTCATC GTCGGGAAGA NCTGCGACAC
            841 CTGCGTCGAA NANGTTGGGG NGNTGGANCA AGANGGANTC CAATTCAGCG GGANCCACCT
            901 GATAGCCTTT GTACTTNATC ANANANTTCN NNCGGNCAAN GATGAAGANN TGNNCNTCTT
            961 CNTCCCNNNN ANCTATGTCT NNNNANNGNN GNNNNNCNTN CNTNNNCNNT NNANNNNNNN
          1021 NGNNGCNTNN NNNANNNNNN NNNN
          //
          Primer: Luc-B_R (Pr0790)
          Region: insert mid(luc 5',hPer3 pro 3')
          Sequence file: RDB15084_A8Ctc.seq check
          >D04733A2_A8Ct_2_Luc-B_R_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNT CTCTTNNTNG CCTTATGCAG TTGCTCTCCN GCGGTTCCNT
             61 CTTCCNGCGG ATAGAATGGC GCCGGGCCTT TCTTTATGTT TTTGGCGTCT TCCATGGTGG
            121 CTTTACCAAC AGTACCGGAA TGCCAAGCTT ACTTAGATCG CAGATCTCGA GGTCTCCGCG
            181 GGGCTCCACG TCGTGCTGGG ACGGCCCGCA GCCTGCTTCT CGCTCACTTT GCAGTCAGTG
            241 ACAGACACCG GTTTCTCACT CCGGTCCCCG CCATGGCGTT AGTGAGAACA GGCGGCCACC
            301 ACGAGCCAGG GGGTCACGCT CATCTCCGAG GAGCTTTTCC CTCCAACATT TTCAAAACCC
            361 GGAGCTCGCT CGACTTTCAG GACTCCGGCC TAGGGGGAGG AGACAAGGGT GACAAGGGAC
            421 AAATGGAACA GACAGTCCGA CGCCAGCGGC CCGGCTGGCT GGGGCGCCCC GACCGCGCGG
            481 TCCTCACCCC TGCCCCAACT TTCCCGCGCG CCGAGGGATG GGGCCCGGGG AGGCGGGTGG
            541 CTCGGAAGCG CCACGCTCCG GGGGCCCCCA GTACCGAGGG CAGGGCTCGC GGGGCTCCTC
            601 TGCCGCGCGC GAGTGCCCAG AACTCCCGCC CGGCCGCCCA GAACTCCCGC CCGGCCGCGC
            661 ACTCACCTGC TGCCCCCGCC CTTGGCCCCG CGCACAGCCG CTCGCCCCCG GGGCCGCCCT
            721 CGCGCAGTCT CGAGGCTCGC CGCGTGCCGG TCAGCAGCCG GCGCCGCGCC CCCTGCCGCC
            781 GCGTGACCGG CCCCGCCCCC GGGCCGCGCG ATTGNTCGTC GGGGCCGACT CCGCANGCCT
            841 CCATTGGCCC TGGGGCGGNG CGCGANCCGG TGCGCCCGGG TTATATNAGC CAGGGCGANG
            901 GGGGCGGGGC CCCCGGGGGC GCGTTACNTN ACGCGCGGGN NCNGNCGGNN GNNGGCGCNN
            961 GGNNNNTCCC ANNNNNCCNG CGGGNGGGNA GGGGNNGGGN NNNNCCNNNN NNNGNNNNNN
          1021 NNNCNNNNNN NNNN
          //
          Primer: Luc_inner_R2 (Pr1286)
          Region: insert mid(luc 5',hPer3 pro 3')
          Sequence file: RDB15084_A8Ctd.seq check
          >D04733A2_A8Ct_2_Luc inner_R2_E03_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCTNNNGNA GGCAGTTCTA TNNNGGNAGA GCNNACACCT
             61 TTAGGCAGAC CAGTAGATCC AGAGGAGTTC ATGATCAGTG CAATTGTCTT GTCCCTATCG
            121 AAGGACTCTG GCACAAAATC GTATTCATTA AAACCGGGAG GTAGATGAGA TGTGACGAAC
            181 GTGTACATCG ACTGAAATCC CTGGTAATCC GTTTTAGAAT CCATGATAAT AATTTTTTGG
            241 ATGATTGGGA GCTTTTTTTG CACGTTCAAA ATTTTTTGCA ACCCCTTTTT GGAAACGAAC
            301 ACCACGGTAG GCTGCGAAAT GCCCATACTG TTGAGCAATT CACGTTCATT ATAAATGTCG
            361 TTCGCGGGCG CAACTGCAAC TCCGATAAAT AACGCGCCCA ACACCGGCAT AAAGAATTGA
            421 AGAGAGTTTT CACTGCATAC GACGATTCTG TGATTTGTAT TCAGCCCATA TCGTTTCATA
            481 GCTTCTGCCA ACCGAACGGA CATTTCGAAG TACTCAGCGT AAGTGATGTC CACCTCGATA
            541 TGTGCATCTG TAAAAGCAAT TGTTCCAGGA ACCAGGGCGT ATCTCTTCAT AGCCTTATGC
            601 AGTTGCTCTC CAGCGGTTCC ATCTTCCAGC GGATAGAATG GCGCCGGGCC TTTCTTTATG
            661 TTTTTGGCGT CTTCCATGGT GGCTTTACCA ACAGTACCGG AATGCCAAGC TTACTTAGAT
            721 CGCAGATCTC GAGGTCTCCG CGGGGCTCCA CGTCGTGCTG GGACGGNCCG CAGCCTGCTT
            781 CTCGCTCACT TTGCANTCAG TGACAGACAC CGGTTTCTCA CTCCGGTCCC CGCCNTGGCG
            841 TTAGTGAGAA CAGGCNGCCA CCACNAGCNA GGGGGTCACG CTCATCTNNN ANGAGCTTTT
            901 NCCTCCNACA TTTTTCNAAA NCCGGANCTC GCTCGANTTT TNNNNNANTN CNGGCCTNNN
            961 GGANGNAGNN NNGGNNNNNN ANNGNANNAA NNNNNNANAN AGNCCGANNN NNNNNGNCCC
          1021 NNNNNNNNNN
          //
          Primer: pAxCALNLF1 (Pr0166)
          Region: insert mid(luc 3')
          Sequence file: RDB15084_A8Cte.seq check
          >D04733A2_A8Ct_2_pAxCALNLF1_H04_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NAGCGGCCGG CCGCCCCGAC TCTNNAATTA
             61 CACGGCGATC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT TTCTTGCGTC
            121 GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC CGCGCAACTT
            181 TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG TCATCGTCTT
            241 TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT CGGGAAGACC
            301 TGCGACACCT GCGTCGAAGA TGTTGGGGTG TTGGAGCAAG ATGGATTCCA ATTCAGCGGG
            361 AGCCACCTGA TAGCCTTTGT ACTTAATCAG AGACTTCAGG CGGTCAACGA TGAAGAAGTG
            421 TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT TGTCAATCAA
            481 GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGAC CTCTCACACA
            541 CAGTTCGCCT CTTTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA CAACCTTCGC
            601 TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT CGGGTGTAAT
            661 CAGAATAGCT GATGTAGTCT CAGTGAGCCC ATATCCTTGC CTGATACCTG GCAGANGGAA
            721 CCTCTTGGCA ACCGCTTCCC CNACTTCCTT ANANANGGGA GCGCCACCAN AAGCNANTTT
            781 CNTGTAAATT NNNATAAATC GTATTTGTCA ATCANAGTGC TTTTTGGNNA NNNANGAGAN
            841 NAGGNNTGGC ACCNNCANCG CACTTTTGNA TCTTGTNATC NNNGAANNNT NCNTCANAAA
            901 CNNCTCTNNN TCAAATCTNT ACATTNAAGA CGANTNNNAA NTNCNNNNTN TCNAATNTNC
            961 NNANNGTANN NAACNTTNCN AAANCNNNGN NNNGANTGGN NNNNNNNNTN AAATCNNNNN
          1021 TATCNNNNNN NGNNTTTGNN TNGNNNAAAA NNNNGNTCTN NNGNNNNNNN NNN
          //
          Primer: Luc inner_R1 (Pr1257)
          Region: insert mid(luc mid)
          Sequence file: RDB15084_A8Ctf.seq check
          >D04733A2_A8Ct_2_Luc inner_R1_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTTTTCCN GGTNNCNNGA TCCNCAACCT TCGCTTCNAA
             61 AAATGGAACA ACTTTACCGA CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT
            121 AGCTGATGTA GTCTCAGTGA GCCCATATCC TTGCCTGATA CCTGGCAGAT GGAACCTCTT
            181 GGCAACCGCT TCCCCGACTT CCTTAGAGAG GGGAGCGCCA CCAGAAGCAA TTTCGTGTAA
            241 ATTAGATAAA TCGTATTTGT CAATCAGAGT GCTTTTGGCG AAGAAGGAGA ATAGGGTTGG
            301 CACCAGCAGC GCACTTTGAA TCTTGTAATC CTGAAGGCTC CTCAGAAACA GCTCTTCTTC
            361 AAATCTATAC ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT
            421 TCCAAAACCG TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT
            481 GCCAAAAATA GGATCTCTGG CATGCGAGAA TCTCACGCAG GCAGTTCTAT GAGGCAGAGC
            541 GACACCTTTA GGCAGACCAG TAGATCCAGA GGAGTTCATG ATCAGTGCAA TTGTCTTGTC
            601 CCTATCGAAG GACTCTGGCA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT
            661 GACGAACGTG TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT
            721 TTTTTGGATG ANTGGGAGCT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA
            781 AACGAACACC ACGGTAGGCT GCGAAATGCC CATACTGTTG AGCAATTCAC GTTCATTATA
            841 AATGTCGTTC GCGGGCGCNA CTGCAACTCC GANAAATAAC GCGCCCNACA CCGGCATAAA
            901 GAATTGAANA NAGTTTTCAN TGCATACGAC GATTCTGTGN NTTGNATTCA GCCNNTANNN
          961 NNTCNTANNN NCTGNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yamaguchi, A., A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans. Sci Rep. 7 (1): 6824 (2017). PMID 28755004.
          original Matsumura, R., Multiple circadian transcriptional elements cooperatively regulate cell-autonomous transcriptional oscillation of Period3, a mammalian clock gene. J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl