Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmRFP-C1-mouse rabphilin

Expression vector of mouse rabphilin, RFP-tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15094_A7F7p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert 5' (mRFP1-Gly linker-rabphilin) Sequence file: RDB15094_A7F7a.seq
>D04592A6_A7F7_2_CMV Forward_D06_12_ABI24.ab1     1 CGTCCCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTATGGCCT   121 CCTCCGAGGA CGTCATCAAG GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCGTGA   181 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA   241 CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC   301 CTCAGTTCCA GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT   361 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG   421 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA   481 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT   541 GGGAGGCCTC CACCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGA   601 TGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCCGA GGTCAAGACC ACCTACATGG   661 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAAGACCGA CATCAAGCTG GACATCACCT   721 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAGCG CGCCGAGGGC CGCCACTCCA   781 CCGGCGCCTC CGGTGGTTCC GGTGGTACCG GTGGATCCAT GACTGACACT GTGGTGAACC   841 GATGGATGTA CCCTGGTGAT GGCCCTCTGC AGTCAAATGA CAAGGAACAG CTGCAGGCAG   901 GATGGTCCGT CCATCCTGGA GCACAGACCG ACAGGCAGAG GAAGCAGGAA GAACTGACAG   961 ACGAGGAGAA GGAGATCATC AACCAGAGTG ATTTGCTCGG GCAGAGAAGA TGGGAAAGCC  1021 ATGGACAGAA CGCATTTGAG CCGCTTGCTT GCACGTTCTT GGAGACCATG AGGAGCATGT 1081 GGCCTGGCAG ATGGCGGTGA ACCGCC //
Primer: mRFP1-C_F Region: insert mid (mRFP1-Gly linker-rabphilin) Sequence file: RDB15094_A7F7b.seq
>D04592A6_A7F7_2_mRFP1-C_F_F11_06_ABI08.ab1     1 TGGCCCTCAG ACCGACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT    61 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCTCCGGTG GTTCCGGTGG   121 TACCGGTGGA TCCATGACTG ACACTGTGGT GAACCGATGG ATGTACCCTG GTGATGGCCC   181 TCTGCAGTCA AATGACAAGG AACAGCTGCA GGCAGGATGG TCCGTCCATC CTGGAGCACA   241 GACCGACAGG CAGAGGAAGC AGGAAGAACT GACAGACGAG GAGAAGGAGA TCATCAACAG   301 AGTGATTGCT CGGGCAGAGA AGATGGAAGC CATGGAACAG GAACGCATTG GGCGCCTGGT   361 GGACCGTCTG GAGACCATGA GGAAGAATGT GGCTGGAGAT GGCGTGAACC GCTGCATTCT   421 GTGTGGGGAA CAGCTGGGTA TGCTGGGCTC GGCCTGTGTC GTGTGTGAAG ACTGTAAGAA   481 GAATGTCTGC ACCAAGTGTG GGGTTGAGAC CTCCAACAAC CGTCCGCATC CGGTATGGCT   541 CTGCAAGATC TGCCTTGAGC AGAGAGAGGT CTGGAAGCGC TCAGGAGCAT GGTTCTTCAA   601 AGGTTTCCCC AAGCAGGTCC TTCCACAGCC CATGCCTATA AAGAAGACCA AGCCCCAGCA   661 GCCTGCTGGT GAACCGGCCA CCCAGGAGCA GCCTACACCT GAGTCCAGGC ATCCAGCCAG   721 GGCTCCAGCT CGAGGTGACA TGGAGGACAG GAGGCCCCCA GGGCAGAAGC CAGGCCCTGA   781 CCTCACCTCT GCTCCTGGGA GAGGAAGCCA TGGGCCTCCC ACGCGTAGGG CCTCTGAGGC   841 ACGGATGAGT ACAGCCGCCC GGGATTCTGA GGGCTGGGAC CATGCCCATG GTGGGGGTAC   901 TGGAGACACC AGCCGTAGCC CAGCAGTTTG AGGCGAGCTA AACTCAGTCC AGGCAGCCCG   961 CCCTGCCCCA GCCCCAGTGC CAAGCCCAGC ACCTCTCAGC CGGTGCAGCC AGGGCCCCCT  1021 GGGGGCAGCA GGCACTCTGG CCAGGGACGC TTTCCGGAGC AGAGCACAGA GCTCTTCAAG  1081 TGACCTGGCC TATTCCAGGG CCTGTCGCCC CAAGCCTGAA GGAAGAACGG ACCTTGCCGG 1141 GGGCCTTCAG CCAGGCGCCC GCCCACACTG TCAG //
Primer: SV40polyA-R Region: insert 3' (rabphilin) Sequence file: RDB15094_A7F7c.seq
>D04592A6_A7F7_2_SV40polyA-R_E06_15_ABI24.ab1     1 AAGAAGTGAG TTTTTAAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG    61 AGTCGCGGCC GCTTAAGGTC AGAGTAAATC TCTTTGTAAA TGTCTCCCCA CACCCACTAC   121 CCAACCTCTG GGGACCAGAG AGGGCTCACA TCCAGACCCT GAAGCCCAGA GATTCTTCTT   181 CTGTAGCAGG ACCTGAAGTG GGGGAGACAT GAAAGGTTCA TAGTTGGCAG GGGAGGCTCT   241 GAAAGGGGAG AGCGGCTGGG GCAGAGACAG GGGACACCAG GGCACCAGGT CCTAATCACT   301 GGACACGTGG TTCTCGTTCT GCAGTTGGTG CCAGCGCTCA ATCTTCTTGT CTTTGTTCTT   361 CAAACACTCA TACCAATGTT TCAAGCGCTC GCCTTTGGCC GAGATCCCCA GCTGGCAGCC   421 TCCGATGTAA TCATTAGACT TGCCAATGTC GTAGTCCCAC ACCGAGATAT CCAGGGACTT   481 TTTAGCCAGG TCGCTGTGTT TGATATCATA AAAGAACTCC TCGTTAAACT CGGGATTCAG   541 GGTCTTCTTT TTAATCTGAG TCTTGTGCTT GGCTTTCTTC CCCATGTCCG GTTTCAGCCA   601 GAGCTTGACA AAGGGGTCTG AGTAGCCGTT GGCATCCATG GCGGCCAGGT GCACACAGCG   661 GATGATTCCC ACAATGAGGC CGCCCTGCTG CGTGCTGTAC ATGAGGGACA CCAGGATCTT   721 GCCCCGTTCC TCTATATCGC CGATCCGCTC TACCTGCTCC TCCTCATAGA GAGCCATGCC   781 ACGGGCCGAC CCGGTGGTCC CTGCTCTCTT CATCGGGATC ACCCGCTCCA GGCAGATGTT   841 GAAGTTTTTC CTCTGGTTAG CCTTCAGCTT CTTGAGCGAG AACCTGGTCT CACCAATGAA   901 CTCGTTGTGG CCAAACTTGT CCTCGTCACA CACGGAGATC CTTAGTGTCT TCCCTCTGCA   961 TGTCCCTCCT CTGTAATGCC CATGATACTG CAGTGTCTCA TTTCCACACA GGTTTCGAGT  1021 GGTGGCGGCA CGTCTTTGTA CGAAGCCTGA TGGACTTGCT GTCTCAGCAG CAGATGAAGC 1081 CTTCACATAG GAATTCTTGC CCAG //
Primer: SV40pro_F_V2 Region: NeoR_KanR Sequence file: RDB15094_A7F7d.seq
>D04592A6_A7F7_2_SV40pro_F_V2_F06_18_ABI24.ab1     1 TACACGGCTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC   901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCCT TCTATGAAAG GTTGGGGCTT   961 CGGAATCGTT TTCCCGGGAC GCCCGGCTGG AATGATCCTC CAGCGCGGGG GATCTCATGG  1021 CTTGCAGGTT CTTCGCCCCA CCCTACGGGA AGCTACTGAA ACACGTTGGA GACCATTACT 1081 GCAGAACCCG CCGCTATGAA CGGGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl