RDB15107 - pBSIISK-SYN-GFP-BGHpA

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pBSIISK-SYN-GFP-BGHpA

Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)).

Catalog number	RDB15107
Resource name	pBSIISK-SYN-GFP-BGHpA
Clone info.	Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)), bovine growth hormone polyadenylation signal (BGHpA).
Vector backbone	pBSIISK (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Ampicillin
Gene/insert name	Human SYN1 genomic DNA
Depositor\|Developer	Hioki, Hiroyuki \|

Other clones in our bank

human SYN1

External Database
Human SYN1

Reference sequence

Sequence (full)	RDB15107zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15107	pBSIISK-SYN-GFP-BGHpA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15107_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: SYN pro, EGFP 5' Sequence file: RDB15107_A7Fda.seq
>D04569A1-2_A7Ed_M13(-40)_G02_20_ABI24.ab1 1 AGCCAAGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG 61 AGGTCCTGCA GAGGGCCCTG CGTATGAGTG CAAGTGGGTT TTAGGACCAG GATGAGGCGG 121 GGTGGGGGTG CCTACCTGAC GACCGACCCC GACCCACTGG ACAAGCACCC AACCCCCATT 181 CCCCAAATTG CGCATCCCCT ATCAGAGAGG GGGAGGGGAA ACAGGATGCG GCGAGGCGCG 241 TGCGCACTGC CAGCTTCAGC ACCGCGGACA GTGCCTTCGC CCCCGCCTGG CGGCGCGCGC 301 CACCGCCGCC TCAGCACTGA AGGCGCGCTG ACGTCACTCG CCGGTCCCCC GCAAACTCCC 361 CTTCCCGGCC ACCTTGGTCG CGTCCGCGCC GCCGCCGGCC CAGCCGGACC GCACCACGCG 421 AGGCGCGAGA TAGGGGGGCA CGGGCGCGAC CATCTGCGCT GCGGCGGACG GTATCGATAA 481 GCTTGATTCG AGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA 541 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG 601 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 661 CCGTGCCCTG CCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT 721 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTTCTG CCATGCCCGA ATGCTACGAT 781 CCAGGAGCGC ACATCTCTTC ATTGTACGAC GGCAACTACA GACTCGAGAA CCCACGGTCC 841 AAAGGTTGGA AAGATTCTAT AGCGACACAC ACCTTGGTTG AATCCCCCAC TCCCAGCCCT 901 CATAGCGATT CGACCTCTCA TGAGGATGAG AGGCGACACT CTCCTGTGGT GTCTCGAGAG 961 CCTCGGATTA TCTAGCGCGC ACCACATCGG TCTTATATGT CTATGAGTCT GGCCAGGCAG 1021 GATCGATGTG ATGCGTGCAG TACGATTCGC CATCAGTACG TACCGATGGT GCCGTACTTA 1081 GCAGCGCG //
Primer: BGH_rev2 (Pr0606) Region: EGFP 3' Sequence file: RDB15107_A7Fdb.seq
>D04569A1-2_A7Ed_BGH_rev2_H02_23_ABI24.ab1 1 CGTCGGGGTG AGTAGCACGG GGGAGGGCAA ACAACAGATG GCTGGCGAAC TAGAAGGCAC 61 AGGAATTCGG TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG 121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG 181 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC 241 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC 301 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG 361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG 421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG 481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG 541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG 601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG 661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG 721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC 781 TTGCTCACCA TGGTGGCTCG AATCAAGCTT ATCGATACCG TCCGCCGCAG CGCAGATGGT 841 CGCGCCCGTG CCCCCCCTAT CTCGCGCCTC GCGTGGTGCG GTCCGGCTGG GCCGGGCGGC 901 GGGCGCGGAC GCGACCAAAG TGGCCCGGGA ATGGGAGTTT GCGGGGGACC GGGCGAGTGA 961 CGTCAGCGCG CCCTTCAGTG CTGAGCGCGG TGGCGCGCGC CGCCAGCGGG GGGGCGAAGC 1021 ACTGTCCGCG GTGCTGAGCT GGCAATTGCG CACGCGCTCG CGCATCTGAT TCCCGTCCCC 1081 GTTCTTCTGG AATAGGGGGA TTGGCCGCCA AATG //
Primer: EGFP-C (Pr0025) Region: BGH p(A) Sequence file: RDB15107_A7Fdc.seq
>D04569A1-2_A7Fd_EGFP-C_A02_02_ABI24.ab1 1 CAGGACTCTC GGGCATGGAC GAGCTGTACA AGTAAATCGA ATTCCTGTGC CTTCTAGTTG 61 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 121 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 181 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 241 GCATGCTGGG GATGCGGTGG GCTCTATGGG GATCCACTAG TTCTAGAGCG GCCGCCACCG 301 CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA 361 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 421 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 481 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 541 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 601 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 661 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 721 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 781 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 841 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 901 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 961 AGCTCACGCT GTAGGTATCT CAGTTCGGGT GTAGGTCGTT TCGCTCCCAA GCTGGGGCTG 1021 TGGTGCACGA ACCCCCCCCG TTCAGCCCGG ACGCCTGCCG CCTTATCCGG TACTATCGTC 1081 CATGAAGTTC GAACCCGGTT AAAGAACCAC CGAAACCTTT AAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl