Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-DEST(r)

Gateway(R) destination vector of AAV2 [reverse].

Catalog number RDB15111
Resource name pAAV2-DEST(r)
Clone info. Gateway(R) destination vector of AAV2 [reverse].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Hioki, Hiroyuki |
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External Database
            Reference sequence
              
             
            Sequence (full) RDB15111zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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            RDB15111 pAAV2-DEST(r) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15111_A7Fdp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ccdB-C_F
            Region: ccdB 3', attR1, AAV2 ITR（R）
            Sequence file: RDB15111_A7Fda.seq check
            >D04569A5-1_A7Fd_ccdB-C_F_D01_04_ABI08.ab1
                1 CTATTGGGGG GGGGGTGAAA ATCGGGGGGA TGAAGCTGGC GCATGATGAC CACCGATATG
               61 GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT
              121 GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA
              181 CACAGCCAGT CTGCAGGTCG ATACAGTAGA AATTACAGAA ACTTTATCAC GTTTAGTAAG
              241 TATAGAGGCT GAAAATCCAG ATGAAGCCGA ACGACTTGTA AGAGAAAAGT ATAAGAGTTG
              301 TGAAATTGTT CTTGATGCAG ATGATTTTCA GGACTATGAC ACTAGCGTAT ATGAATAGGT
              361 AGATGTTTTT ATTTTGTCAC ACAAAAAAGA GGCTCGCACC TCTTTTTCTT ATTTCTTTTT
              421 ATGATTTAAT ACGGCATTGA GGACAATAGC GAGTAGGCTG GATACGACGA TTCCGTTTGA
              481 GAAGAACATT TGGAAGGCTG TCGGTCGACT AAGTTGGCAG CATCACCCGA AGAACATTTG
              541 GAAGGCTGTC GGTCGACTAC AGGTCACTAA TACCATCTAA GTAGTTGATT CATAGTGACT
              601 GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC TAATTTAATA
              661 TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA CTTGTCACGT
              721 GCGGACCGAG CGGCCGCAGG AACCCCTAGT GATGGGAGTT GGCCACTCCC TCTCTGCGCG
            781 CTC
            //
            Primer: Cm_M_R
            Region: CmR 5', cat pro, attR2, AAV2 ITR (L)
            Sequence file: RDB15111_A7Fdb.seq check
            >D04569A5-1_A7Fd_Cm_M_R_C01_03_ABI08.ab1
                1 TTGAGGAGGG GGAAACGTAT TCCCATTATT CACCAGCTCA CCGGTCTTTC ATTGGCCATA
               61 CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
              121 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
              181 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
              241 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
              301 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
              361 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AAAAGTTGGC CCAGGGCTTC
              421 CCGGTATCAA CAGGGACACC AGGATTTATT TATTCTGCGA AGTGATCTTC CGTCACAGGT
              481 ATTTATTCGG CGCAAAGTGC GTCGGGTGAT GCTGCCAACT TAGTCGACTA CAGGTCACTA
              541 ATACCATCTA AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
              601 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
              661 CGTTCAGCTT TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG
            721 TTGGCCCACT CCCTCTCTGC GCGCTCG
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl