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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

YC2.60/pRSETB

Yellow cameleon calcium ion sensor clone YC2.60.

Catalog number RDB15134
Resource name YC2.60/pRSETB
Clone info. Calcium ion sensor clone YC2.60 Yellow cameleon having wild-type CaM and the circular permutated cp173Venus. Bacterial expression plasmid.
Comment Known differences (at least): C to T at 291nt (97aa, Arg, synonym), T to C at 438nt (146aa, Tyr, synonym), A to C at 493nt (165aa, Asn to His), G to C at 639nt (213aa, Lys to Asn) of AB178711.1. Nucleotide sequence of AB178711.1 will be updated.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB178711 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
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External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
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        提供条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
        Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
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        備考 提供条件は英文のまま記載してください。

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        RDB15134 YC2.60/pRSETB DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The YC2.60/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15134).

        Reference section:

        Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., Miyawaki, A., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


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        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15134_A7H9p1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer:
        Sequence file: RDB15134_A7H9c.seq check
        >15134_15134_B2CuF_YC4.6-M_R_H03_24_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTCN NTTGCCATCA GCATCGACTT CATTGATCAT
           61 ATCCTGCAAT TCTGCTTCCG TTGGGTTTTG TCCAAGCGAC CTCATAACGG TGCCAAGTTC
          121 CTTTGTGGTG ATGGTGCCGT CCCCATCCTT GTCGAATAAT GAGAAGGCTT CTTTGAACTC
          181 TGCAATCTGC TCTTCTGTCA GTTGGTCATG CATGCGGGCG GCGGTCACGA ACTCCAGCAG
          241 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA
          301 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC
          361 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTGGG CCTTGATGCC
          421 GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT
          481 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC
          541 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA
          601 GAAGANGGTA CGCTCCTGNA NGTAGCCTTC NGGCATGGCG GANTTGAANA ANTCNNGCTG
          661 CTTCNTGTGG TCNGGGTANC GGCTGNNNNA CTGNNCNCCC CNNGNNNNNN NGNNCNNNNN
        721 GNNNGGNNNN NGNNNNNNGN NNNNNNNNN
        //
        Primer:
        Sequence file: RDB15134_A7H9d.seq check
        >15134_15134_B2CuF_YC2.6-M_F_C01_03_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNN NNANTTACTT TCCTGNATTT CTTACTATGA
           61 TGGCTAGAAA AATGAAGGAC ACAGACAGCG AAGAGGAAAT CCGAGAAGCA TTCCGTGTTT
          121 TTGACAAGGA TGGGAACGGC TACATCAGCG CTGCTGAATT ACGTCACGTC ATGACAAACC
          181 TCGGGGAGAA GTTAACAGAT GAAGAAGTTG ATGAAATGAT AAGGGAAGCA GATATCGATG
          241 GTGATGGCCA AGTAAACTAT GAAGAGTTTG TACAAATGAT GACAGCAAAG GGGGGGAAGA
          301 GGCGCTGGAA GAAAAACTTC ATTGCCGTCA GCGCTGCCAA CCGGTTCAAG AAGATCTCCA
          361 GCTCCGGGGC ACTGGAGCTC ATGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
          421 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
          481 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
          541 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GGGTGGCAGC GGTGGCATGG
          601 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
          661 ACGTAAACGG NCACAAGTTC AGCGTGTCCG GCGAGGGCGA NGGCGATGCC ACCTACNGCA
          721 AGCTGANCCT GAANCTGANC TGNNCCACCG GCAAGCTGCC CGTGCCCNNN CCNNCCCTCG
          781 TGNNNNCCCT GGGCTANNNC CTGCANNGCT NCNCCNNNTN NCCCGANCAC NTGAAGCANC
        841 ACNACNNCTT NNAGNNCNNN NNGNCNNANN NNNACNNCNN NNNNNNNN
        //
        Primer:
        Sequence file: RDB15134_A7H9e.seq check
        >15134_15134_B2CuF_YC4.6-M_F_A01_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNC NNNATGANGT CGCTTGGACN AAACCCAACG GAAGCAGAAT
           61 TGCAGGATAT GATCAATGAA GTCGATGCTG ATGGCAATGG AACGATTTAC TTTCCTGAAT
          121 TTCTTACTAT GATGGCTAGA AAAATGAAGG ACACAGACAG CGAAGAGGAA ATCCGAGAAG
          181 CATTCCGTGT TTTTGACAAG GATGGGAACG GCTACATCAG CGCTGCTGAA TTACGTCACG
          241 TCATGACAAA CCTCGGGGAG AAGTTAACAG ATGAAGAAGT TGATGAAATG ATAAGGGAAG
          301 CAGATATCGA TGGTGATGGC CAAGTAAACT ATGAAGAGTT TGTACAAATG ATGACAGCAA
          361 AGGGGGGGAA GAGGCGCTGG AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA
          421 AGAAGATCTC CAGCTCCGGG GCACTGGAGC TCATGGACGG CGGCGTGCAG CTCGCCGACC
          481 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
          541 TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
          601 TGGAGTTCGT GACCGCCGCC NGGNTCACTC TCGGNATGGA CNANCTGTAC NAGGGTGGCA
          661 GCGGTGGCAT GGTGAGCAAG GGCGANGANC TGNTCNCCNG GGNGGNGNCN NNNCNGGTCN
        721 ANCNNNNNNG NNNNNNAAAC NGNNN
        //
        Primer:
        Sequence file: RDB15134_A7H9f.seq check
        >15134_15134_B2CuF_T7_20bp_A01_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNTTGT TNNNCTTTAA GAAGGAGNNA TACATATGCG GGGTTCTCAT
           61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
          121 TACGACGATG ACGATAAGGA TCCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
          181 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAGGTTCAG CGTGTCCGGC
          241 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
          301 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTGGGGCGT GCAGTGCTTC
          361 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
          421 TACGTCCAGG AGCGTACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
          481 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
          541 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACATCAGCCA CAACGTCTAT
          601 ATCACCGCCG ACNAGCAGAA GANCGGCATC NAGGCCCACT TCNAGANCCG CCACAACATC
          661 NAGGNNGGCA GCGNGCAGCT CGNNGANCAC TACCAGCAGA ANNCCCCNNT CNGNNNNNGN
        721 CCCNNNCTNC NNNCNNNNNN NNNN
        //
        Primer:
        Sequence file: RDB15134_A7H9g.seq check
        >15134_15134_B2CuF_T7ter_R1_B01_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNCNN NNTNNTTGCT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT
           61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTCGATGTT GTGGCGGATC
          121 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
          181 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
          241 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
          301 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
          361 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
          421 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
          481 AGCTTCAGGG TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC
          541 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC
          601 TCCTCGCCCT TGCTCACCAT GCCACCGCTG CNACCCTTGT ACAGCTCGTC CNTGCCGAGA
          661 GTGATCCCNG CGGCGGTCAC NAACTCCAGC NNNNCATGNG ATCGCGCTTC NCNNTNGGGN
        721 NCTTNNNTCN NNNNNGNNNN NNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]

        2024.03.12

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