Please review the QC test results indicated by icon below as well as clone information before placing your order.

YC4.60/pRSETB

Yellow cameleon calcium ion sensor clone YC4.60.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.

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提供条件	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested. Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15138_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15138_A7H9a.seq
>15138_15138_B0KrF_lacIpro-B_F_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCGNNTCN GTGNGCGAGG AAGCGGAAGA NCGCCCNATA    61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGATCTC GATCCCGCGA   121 AATTAATACG ACTCACTATA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT   181 AACTTTAAGA AGGAGATATA CATATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG   241 CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC   301 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG   361 ACGGCGACGT AAACGGCCAC AGGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT   421 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA   481 CCCTCGTGAC CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA   541 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGTACCATCT   601 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC   661 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA NGACGGCAAC ATCCTGGGGC   721 ACAAGCTGGA GTACAACTAC ATCAGCCACN ACGTCTATAT CACCGCCGAN NAGCAGAAGA   781 ACGGCATCAA GGCCCACTTC AAGATCCGCC ACAACATCGA GGANNGCAGC GNGCAGCTCG   841 CCNANCACTA CNNGCAGAAC ACCCCCATCG GCGACNGNCC CNNGCTGCTG NCCGANAACC   901 NCTACCNGAN CANCCAGNNC GCCCNNANCA AAGANCCCNA NNANNANCNN GNTCACATGG 961 NNCNGCNNGN NNTNNNNGNN CNGNNNNCNN CNNNNNNNNN CNNNNNNNNN N //
Primer: T7ter_R1 (Pr0798) Region: insert 3' Sequence file: RDB15138_A7H9b.seq
>15138_15138_B0KrF_T7ter_R1_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGTNNNT GCTNNGCGGT GGCAGCAGNN NNNTCAGCTT    61 CCTTTCGGGC TTTGTTAGCA GCCGGATCAA GCTTCGAATT CTTACTCGAT GTTGTGGCGG   121 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG   181 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG   241 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC   301 TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG   361 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG   421 CCGTAGCCCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG   481 ATCAGCTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG   541 AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC   601 AGCTCCTCGC CCTTGCTCAC CATGCCACCG CTGCCACCCT TGTACAGCTC GTCCATGCCG   661 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG   721 GGGTCTTTGC TCANGGCGGA CTGGTAGCTC ANGTAGTGGT TGTCGGGCAG CAGCACGGGG   781 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCATG   841 ANCTCCAGNG CCCCGGANCT GGANATCTTC TTGNNCCGGT NGGCAGCGCT GANGGCAATG   901 AAGTTTTTCT TCCANCGCCT CTTCCCCCCC NNTTGCNGNN NTCATTTGNA CAAACTNTTC   961 ATANNTTNNN NTNGGNNCAT CACCNNTCNN NNATNNNNNN NCNNTNNNNN NTTNNNNNAN 1021 NNNNNNNNN //
Primer: FP-N_F1 (Pr0920) Region: insert mid Sequence file: RDB15138_A7H9c.seq
>15138_15138_B0KrF_FP-N_F1_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGNAGCA GCNCGACTTC TTNNNNNNNC    61 GCCNNTGCCC GAAAGGCTAC GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT   121 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA   181 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA   241 TCAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA   301 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA   361 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG   421 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG   481 CCGCCCGCAT GCATGACCAA CTGACAGAAG AGCAGATTGC AGAGTTCAAA GAAGCCTTCT   541 CATTATTCGA CAAGGATGGG GACGGCACCA TCACCACAAA GCAGCTTGGC ACCGTTATGA   601 GGTCGCTTGG ACAAAACCCA ACGGAAGCAG AATTGCAGGA TATGATCAAT GAAGTCGATG   661 CTGATGGCAA TGGAACGATT TACTTTCCTG AATTTCTTAC TATGATGGCT AGAAAAATGA   721 AGGACACAGA CAGCGAAGAG GAAATCCNAG AAGCATTCCG TGTTTTTGAC AAGGATGGGA   781 ACGGCTANNN TCAGCGCTGC TGAATTACGT CACGNNNNNN NNAACCTCGG GGGANAAGTT   841 AACAGATGAA GAAGTTGATG AAATGATAAG GGAANCAGAT ATCGATGGTG ATGGCCNAGT   901 AAACTATGAN NAGTTTGTAC NAATGATGAN NGCAAANNGG GGGGGGAANN NGNGCTNGNA   961 AGAAAAANNN TTCNTTGNCN GNNCNNNCNC NNNCNNANCN NGNNTNCAAN NANNNANCNN 1021 CCNNNCNNNN NNGNNNNNNN GNANNNNNNN NNGNNNNNNN NNN //
Primer: YC4.6-M_F (Pr0721) Region: insert mid Sequence file: RDB15138_A7H9d.seq
>15138_15138_B0KrF_YC4.6-M_F_D08_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GANGTCGCTT GGNNNAAAAC CCNACGGANN    61 NNGAATTGCA GGATATGATC AATGAAGTCG ATGCTGATGG CAATGGAACG ATTTACTTTC   121 CTGAATTTCT TACTATGATG GCTAGAAAAA TGAAGGACAC AGACAGCGAA GAGGAAATCC   181 GAGAAGCATT CCGTGTTTTT GACAAGGATG GGAACGGCTA CATCAGCGCT GCTGAATTAC   241 GTCACGTCAT GACAAACCTC GGGGAGAAGT TAACAGATGA AGAAGTTGAT GAAATGATAA   301 GGGAAGCAGA TATCGATGGT GATGGCCAAG TAAACTATGA AGAGTTTGTA CAAATGATGA   361 CAGCAAAGGG GGGGAAGAGG CGCTGGAAGA AAAACTTCAT TGCCGTCAGC GCTGCCAACC   421 GGTTCAAGAA GATCTCCAGC TCCGGGGCAC TGGAGCTCAT GGACGGCGGC GTGCAGCTCG   481 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC   541 ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG   601 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG   661 GTGGCAGCGG TGGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC   721 TGGTCGANCT GGACGGCGAC GTAAACGGNC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG   781 GCGANGCCNC CTACNGCAAN NCTGACCCTG AAGCTGATCT GCNNCACCGG CAAGCTGCCC   841 GTGCCCTGNC CCNNCCCTCG NGACCACCCT GGGCTACNNN CNGCAGNGCT NCNCCCGCNA   901 CCCCGACCAC ATGNANNCAG CNNGANTTCT TCNNNNTCCG CCNTGNNCGA AGGNTACNNN 961 NNNNGNANCG NANNNNCTTC TTCNNNGNNN NNNNGNNANC TANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl