Please review the QC test results indicated by icon below as well as clone information before placing your order.

Flash pericam/pcDNA3

Calcium ion sensor clone Flash pericam having a circularly permuted YFP and a CaM.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15139_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, T7 pro, insert 5' Sequence file: RDB15139_A7H9a.seq
>D04498C6_A7H9_1_CMV-Forward_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNG NNGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA   121 CGACTCACTA TAGGGAGACC CAAGCTTGCG GCCGCCACCA TGGTGAAGAG GCGCTGGAAG   181 AAAAACTTCA TTGCCGTCAG CGCTGCCAAC CGGTTCAAGA AGATCTCCAG CTCCGGGGCA   241 CTGGGGTCTG CAGGCTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC   301 GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC   361 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC   421 TACCTGAGCC ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC   481 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT   541 GGCAGCGGTG GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG   601 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC   661 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG   721 CCCTGNCCCA CCCTCGTGAN CACCTTCGGC TACGGCCTGA AGTGCTTCGC CCGCTACCCC   781 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAN   841 CGCACCATCT TCTTCNAGGA CGACGGCAAC TACAAGANCC GCGCCGANGT GAANTNCGAG   901 GGCGACACCC NGGNGAACCG CATCGAGCTN AANGGCATCG NNTTCNAGGA NGNNNGNANC 961 NTCCNNGGGN NNNNNCNNGN NNTANNNCNG NNACNNGNNN GNNNNNNCNN NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: insert 3' Sequence file: RDB15139_A7H9b.seq
>D04498C6_A7H9_1_BGH_rev2_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNANNN NNNNNNGGCT GGCAANNNNN    61 NNGNACAGTC GAGGNNNNNN NNNNNNNCTC TAGCATTTAG GTGACACTAT AGAATAGGGC   121 CCNCTANATG CATGCTCGAG CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCTTACTTT   181 GCTGTCATCA TTTGTACAAA CTCTTCATAG TTTACTTGGC CATCACCATC GATATCTGCT   241 TCCCTTATCA TTTCATCAAC TTCTTCATCT GTTAACTTCT CCCCGAGGTT TGTCATGACG   301 TGACGTAACT GAGCAGCGCT GATGTAGCCG TTCCCATCCT TGTCAAAAAC ACGGAATGCT   361 TCTCGGATTT CCTCTTCGCT GTCTGTGTCC TTCATTTTTC TAGCCATCAT AGTAAGAAAT   421 TCAGGAAAGT AAATCGTTCC ATTGCCATCA GCATCGACTT CATTGATCAT ATCCTGCAAT   481 TCTGCTTCCG TTGGGTTTTG TCCAAGCGAC CTCATAACGG TGCCAAGTTC CTTTGTGGTG   541 ATGGTGCCGT CCCCATCCTT GTCGAATAAT GAGAAGGCTT CTTTGAACTC TGCNNNNNNC   601 TCTTCTGTCA NTTGGTCATG CATCCCNGNN NNGTTGTACT CCAGCTTGTG CCCCANNNTG   661 TTGCCGTCCT CCTTGAANTC GATGCCCTTC AGCTCGATGC GGTTCNCCAG GGTGTCGCCC   721 TCNAACNNNN NNTCNGCGCG GGTCTTGTAN TTGNNNNCGT CCTTGAAGAA GANGGGTGCG   781 CTCCTGGACN NNNNNCTTCN GGNNNTGGCG GNACTTGAAG AANNTCGTGC TGCTTCATGT   841 GGTCNGGGGT ANCGGGCGAA GCACTTCANG NCNGNANCCN NNNNGNGGTC ACNNANGGTG   901 GGCNNNGGCA CGGGCAGCTT GCCGGTGGTG CANNNGAAAC TTCNNGGGTC NNCNNGNCNG   961 NANGNNNCNN CNCCCTNNNC CTCNCNNNAN NNNCTGAACT TGNGGNCNNN TTTANNNNNN 1021 NNNGNNNNNN NNNN //
Primer: EGFP-N_F (Pr0649) Region: insert mid Sequence file: RDB15139_A7H9c.seq
>D04498C6_A7H9_1_EGFP-N_F_G07_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CNNNGGCGNN GCNNCCTACG GCAAGCTGNN CCTGAAGTTC    61 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTCGGCTAC   121 GGCCTGAAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC   181 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC   241 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG   301 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACGGTACC   361 GGGATGCATG ACCAACTGAC AGAAGAGCAG ATTGCAGAGT TCAAAGAAGC CTTCTCATTA   421 TTCGACAAGG ATGGGGACGG CACCATCACC ACAAAGGAAC TTGGCACCGT TATGAGGTCG   481 CTTGGACAAA ACCCAACGGA AGCAGAATTG CAGGATATGA TCAATGAAGT CGATGCTGAT   541 GGCAATGGAA CGATTTACTT TCCTGAATTT CTTACTATGA TGGCTAGAAA AATGAAGGAC   601 ACAGACAGCG AAGAGGAAAT CCGAGAAGCA TTCCGTGTTT TTGACAAGGA TGGGAACGGC   661 TACATCAGCG CTGCTCAGTT ACGTCACGTC ATGACAAACC TCGGGGAGAA GTTAACAGAT   721 GAAGAAGTTG ANGAAATGAT AANGGAAGCA GATATCGATG GTGATGGCCA AGTAAACTAT   781 GAAGAGTTTG TACNAATGNN GACAGCAAAG TAAGAATTCT GCAGATATCC ATCACACTGG   841 CGGNNGCTCN NNCATGNATC TANAGGGNCC TATTCTATAN TGTCANNNAA ATGCTANNNC   901 TCGNNNNNCA NCNNCNNNNG NNCNNTCTAN NNNNNANCNN TCTNNNNNTN GNCCNNNCCC 961 NNNNNNNNNC NNTNNNNCNN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: NeoR Sequence file: RDB15139_A7H9d.seq
>D04498C6_A7H9_1_SV40pro_ori_F_H07_22_ABI24.ab1     1 NNNNNNNNNN NNNGNCGCCT CNGCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA   121 TCAAGAGACA GGNTGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC   181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG   241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC   301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC   361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG   421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA   481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG   541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG   601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT   661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGNNCTC GTCGTGACCC ATGGCGATGC   721 CTGCTTGCCG AATATCATGG TGGANNATGG CCGCTTTTCT GGANTCATCG ACTGTGGCCG   781 GCTGNNTGTG GCGGANCGCT ATCANGNCAT ANCGTTGGCT ANCCGTGATA TTGCTGAAGA   841 NCTTGGCGGC GAATGGGCTG ANCGCTTCCT CGNNCTTTAC NGNANCGCNN CNCCNNATTN 901 NCANCNNATC NNCNTTCNNN CGNCNTTNNN GNCNANNTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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