Please review the QC test results indicated by icon below as well as clone information before placing your order.

Ratiometric pericam/pcDNA3

Calcium ion sensor clone Ratiometric pericam having a circularly permuted YFP and a CaM.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15143_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Sequence file: RDB15143_A7H9a.seq
>D04498D1_A7H9_1-CMV-Forward_G07_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNGGCGGT AGGCNNTGTA NGGTGNNNNG    61 TCTATATAAG CAGAGCTCTC TGGCTAACTA NANANCCCAC TGCTTACTGG CTTATCGAAA   121 TTAATACGAC TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACCATGAAG   181 AGGCGCTGGA AGAAAAACTT CATTGCCGTC AGCGCTGCCA ACCGGTTCAA GAAGATCTCC   241 AGCTCCGGGG CACTGGGGTC TGCAGGCTAC AACAGCGACA ACGTCTATAT CATGGCCGAC   301 AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC   361 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG   421 CCCGACAACC ACTACCTGAG CTTCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC   481 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG   541 CTGTACAAGG TCGACGGTGG CAGCGGTGGC ACCGGTGTGA GCAAGGGCGA GGAGCTGTTC   601 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC   661 GTGTCCGGCG AGGGCGNNGG CGATGCCACC TACGGCAAGC TGACCCTGAA NNNCNNNNGC   721 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCNNGA CCACCTTCGG CTACGGCCTG   781 AANTGCTTCN CCCGCTACCC CGACCACATG AAGCANCANN ACTTCTTCAA NNTCCGCCAT   841 GCNNNNNGGC TACNTCCANG NANCGCACCA TCTTCTNNAA GGACNANGGC NANNNNCNAG   901 ACCCGCGCCN ANNNNAAGTT CNAGGGCGAN ANCCTGGTGA ANNNCNTCNN NCTNANNGGC   961 ATCGNNNTNN ANGNNNNANN GGCNACNNNC NGGGGCNNAA NNCTNGNNNN TANNANNGGT 1021 ACCNNANCNN NCNNNNNNNA NNANNNNANN NNNNNNNN //
Primer: BGH_rev2 Sequence file: RDB15143_A7H9b.seq
>D04498D1_A7H9_1-BGH_rev2_H07_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNNGNNGGG NAAACNACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT   121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATATCTG CAGAATTCTT ACTTTGCTGT   181 CATCATTTGT ACAAACTCTT CATAGTTTAC TTGGCCATCA CCATCGATAT CTGCTTCCCT   241 TATCATTTCA TCAACTTCTT CATCTGTTAA CTTCTCCCCG AGGTTTGTCA TGACGTGACG   301 TAACTGAGCA GCGCTGATGT AGCCGTTCCC ATCCTTGTCA AAAACACGGA ATGCTTCTCG   361 GATTTCCTCT TCGCTGTCTG TGTCCTTCAT TTTTCTAGCC ATCATAGTAA GAAATTCAGG   421 AAAGTAAATC GTTCCATTGC CATCAGCATC GACTTCATTG ATCATATCCT GCAATTCTGC   481 TTCCGTTGGG TTTTGTCCAA GCGACCTCAT AACGGTGCCA AGTTCCTTTG TGGTGATGGT   541 GCCGTCCCCA TCCTTGTCGA ATAATGAGAA GGCTTCTTTG AACTCTGCAA TCTGCTCTTC   601 TGTCAGTTGG TCGGTACCGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT   661 GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC   721 GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAANATG GTGCGCTCCT GGACGTAGCC   781 TTCGGGCATG GCGGANTTGA ANAAGTCNTG CTGCTTCATG TGGTCNGGGT ANCGGGCGAA   841 GCACTTCANN CCGTAGCCGA ANGTGGTCNN GANGGTGGGC CANGGCACGG GCAGCTNGNN   901 NGTGGTGCAG ANGANCTTCN NGGTCAGCTT GCCGTANGTG GCATCGCCNN CGCCNNNNNN   961 NGANNNGCTG AACTTGNGNN NNTTNANNNC NNCNNNCCAN CTNNNNCANG ANNGGNCNNN 1021 NNNNNNN //
Primer: pricam-M_R Sequence file: RDB15143_A7H9c.seq
>15143_15143_B1IlF_pericam-M_F_E02_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGCCCTTC NGCTCGATGC GGTTCNCCAG GGTGTCGCCC    61 TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC   121 TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG   181 GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG TCACGAGGGT GGGCCAGGGC   241 ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG   301 CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC   361 AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACACCGGT GCCACCGCTG   421 CCACCGTCGA CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC   481 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGAAG   541 CTCANGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG   601 TANTGGTCGG CGANCTGCAC GCCGCCGTCC TCGATGTTGT GNNGGANCTT GAAGTTGGNC   661 TTGATGCCGT TCNTCTGCNN GNCNGNCNNN GNNATANACN TTGNCNNTNN NNNANNNNNN 721 NANCCNNNNN NCNNNNNNNN NN //
Primer: SV40pro_F Sequence file: RDB15143_A7H9d.seq
>D04498D1_A7H9_1-SV40pro_F_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNANN NGTGANGNNG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA    61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT   121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT   181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT   241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA   301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC   361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG   421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC   481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA   541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA   601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC   661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA   721 AAATGGCCGC TTTTCTGGAN TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA   781 NGACATAGCG TTGGCTACNC GTGATATTGC TGAAGANCTT GGCGGCGAAT GGGCTGANCG   841 CTTNCTCGNG CTTTACNGNA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT   901 TNNNNNCGAN TTCNTCNGAN CGGNNNNNNG GGNNTCNAAN TNANNNANNN AGCNNNNCCN 961 NNNCNNNNCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl