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Expression vector of rat Endoplasmin (ENPL), mCherry-ER retention signal (KDEL) tag.
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External Database rat Endoplasmin Reference sequence |
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| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016) |
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| Ordering | Order form [Credit Card Payment ] [Bank Transfer Payment]In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。 |
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| 提供依頼 | 提供依頼書 [Word ]本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB15154 | ENPL-mCherry-KDEL | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The ENPL-mCherry-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15154). |
Reference section:
| Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB15154_A7Flp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB15154_A7Fla.seq
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>D04425A3_A7Fl_2_CMV-Forward_A08_02_ABI24.ab1 1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGGCC 121 ACCATGAGGG TCCTGTGGGT GCTGGGCCTC TGCTGCGTCC TGCTGACCTT CGGGTTTGTG 181 AGAGCTGACG ATGAAGTCGA TGTGGATGGC ACGGTAGAAG AGGACCTGGG TAAAAGTAGA 241 GAAGGCTCAC GGACAGACGA TGAAGTTGTG CAGAGAGAGG AAGAAGCTAT TCAGTTGGAT 301 GGTTTAAATG CATCACAAAT AAGGGAACTT AGAGAAAAAT CGGAAAAGTT CGCCTTCCAA 361 GCTGAAGTTA ACAGGATGAT GAAACTTATC ATCAATTCTT TGTATAAAAA TAAAGAGATT 421 TTCCTGAGAG AACTGATTTC AAATGCTTCT GATGCTTTAG ACAAGATAAG GCTCATCTCA 481 CTAACTGATG AAAATGCACT TGCTGGAAAT GAGGAGTTGA CGGTCAAGAT TAAGTGTGAC 541 AGAGAGAAAA ACCTGCTGCA TGTCACAGAC ACGGGTGTAG GAATGACCAG AGAGGAGCTG 601 GTTAAAAACC TGGGCACCAT AGCCAAATCT GGAACCAGCG AGTTTCTAAA CAAAATGACA 661 GAAGCACAAG AAGACGGTCA GTCAACTTCC GAACTGATCG GCCAGTTTGG TGTCGGTTTT 721 TATTCTGCCT TCCTTGTAGC AGATAAGGTC ATTGTCACGT CGAAACACAA CAATGATACC 781 CAGCACATCT GGGAATCCGA CTCTAATGAA TTCTCTGTGA TTGCTGACCC AAGAGGAAAC 841 ACACTAGGTC GTGGAACAAC GATTACTCTT GTCTTAAAAG AAGAAGCATC TGATTACCTT 901 GAATTGGATA CAATTAAAAA ATCTCGTCAG AAAGTATTCT CAGTTTCATC AACTTTCCAT 961 CTATGTGTGG AGTAGCAAGA CTGAGACTGT GGAGGAGCCC TTTGGTAGAG AACGAAACCA 1021 GCACCAGAGA GATGGAAGCA GCCTGAATGA ATGGAGCCGG CAGTAGAGAG GAGGAGGAAG 1081 AGTAGAGGCC AAGACGAAGA // |
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Primer: mCherry-N_rev Sequence file: RDB15154_A7Flb.seq
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>D04425A3_A7Fl_2_mCherry-N_R_B08_05_ABI24.ab1 1 TCCATACTCT TGATGATGGC CATGTTATCC TCCTCGCCCT TGCTCACCAT GGTGGCGACC 61 GGTGGATCCC GGGCCCGCGG CAATTCATCC TTCTCTGTAG GTTCCTTTTC TGTTTCCTGT 121 TCTTCTTCTT CTTCTTCTGC CCCTGCGTCT GTCTCTTCTT CATCCTGCTC GGAGTCATCT 181 GTGGTGTCTT CTGTGGTGTC TTCAGGCTCC TCCTCTGGCT CTTCCTCTAC CTGTGCTTCA 241 GGGTCAATGT TTAAGCTGAG GCGGAGCATC CTTTCTATCC TGTCTCCATA TGCCTTGGTG 301 TCGGGTAGAA GGTATCCTGA CCGAAGAGTT GCCGTTTCAA ACAAAACTAC AGCAAGATCC 361 ATGACGGTCT TGTCATCTTC ATCTTCCTTA ACCCTCCGCA GCATGTCCCT GATCAGTGGG 421 TGTCTAGGAT TAATTTCAAA CGTTTTCTTC TGACTGGCAT AGTAATTTGT AGAGATGTCC 481 TTGCCCGTCT GGTATGCTTG TGCCTTCATG ATCCTCTCCA TGTTTCCAGA CCATCCATAC 541 TGGCTGGCCA CAAGGGCACA GGGAGACTCT GTGAGGCGCT GGGACACCAC AGCCTTTTCG 601 ATCTTGTCCT TGAGGGCCTT ATCTTTCATC CAGTTGAGCA GAGGCTCAAA CTCCTTCTCT 661 GTTGCTTCCC GACTTTCTTT ACTTTTCTCA CTCTCATCAA ACTTCACTCC TTCTTTGGCA 721 ACATTCTGGA ACCGTTTCCC ATCAAACTCA GGAAGAGCCT GAATGCAGTA TTCATCCACG 781 GGCTCTGTGA GGTAAATAAC TTCATAGCCC TTCTTCAGAA GCCTCTCAAC AAACGGAGAA 841 GATTCAGCCT CTTTTCTGCT TGACCCAGCC ATGAAGTAGA TTTTGTCCTG TTTTTCCTTC 901 ATTCTTTCCA CATACTGGTC TAAACTAGTA ATGTCAGTTG AAATGGGTGA GAAGACTGGG 961 AAACCTAAGA AGTTTAGCAA AGCCGTGTTT CGATTTGAGT GATCTTTCAA ATCACACCCA 1021 AAGCTTTGAT ATTGGTGCCC AAAATTCTTT TCCAGGAAAG TTGTTCATTA TATTTTCTCA 1081 ATCAGTCGAA TCCATCCTTG GATCAATGGT CCAGAAGTTT TGGCGGGTC // |
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Primer: SV40polyA-R Sequence file: RDB15154_A7Flc.seq
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>D04425A3_A7Fl_2_SV40polyA-R_C08_08_ABI24.ab1 1 AAAAAGGGGG AGTTTTTAAG CAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT 61 AGAGTCGCGG CCGCTAGAGC TCATCCTTCT TGTACAGCTC GTCCATGCCG CCGGTGGAGT 121 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAGGTGA 181 TGTCCAACTT GATGTTGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT 241 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA 301 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC GGAGGAGGCC TCCCAGCCCA 361 TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT 421 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT 481 CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT 541 CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA 601 GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG 661 TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG 721 AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG TTATCCTCCT 781 CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC CCGCGGCAAT TCATCCTTCT 841 CTGTAGGTTC CTTTTCTGTT TCCTGTTCTT CTTCTTCTTC TTCTGCCCCT GCGTCTGTCT 901 CTTCTTCATC CTGCTCGGAG TCATCTGTGG TGTCTTCTGT GGTGTCTTCA GGCTCCTCCT 961 CTGGCTCTTT CCTCTACCTG TGCTTCAGAT CAATGTTTAA GCTGGAGCGG AAGCCATCCC 1021 TTTCTATCCT GTCTCCATAT GCCCTTTGAG TCGGGTAGAA AGTATCTTGA CGGAAGAGGT 1081 TGCCGGTTTC AAACCAGA // |
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Primer: SV40pro_F_V2 Sequence file: RDB15154_A7Fld.seq
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>D04425A3_A7Fl_2_SV40pro_F_V2_D08_11_ABI24.ab1 1 GACCGGGCTT TTTTGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GGTTGGGGCT 961 TCGGAATCGT TTTCCCGGGA CGCCCGGCTG GGAATGATCC CTCCAGCGCG GGGATCTCAT 1021 TGCTGAAGTC TTCGCCACCC TAGGGAGCCT ACTGAACACG GAAGGAGACC ATTACTGGAA 1081 GGACTCGCGC TATGAACGGT CAATAT // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP] |
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2023.12.13
GNP_filter3_RDBDEP_html_231213.pl