Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-FLAG-mouse syntaxin-1a

Expression vector of mouse syntaxin-1A [Stx1a in NCBI].

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 277 (33), 30351-30358, 2002)。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15166_A7Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF1a pro, FLAG tag, insert 5' Sequence file: RDB15166_A7Fma.seq
>D04709A1_1_A7Fm_EF1a-C_F_A01_01_ABI24.ab1     1 TAATGGATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC    61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT   121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCTATGAAGG ACCGAACCCA   181 GGAGCTCCGC ACGGCCAAGG ACAGCGATGA CGACGACGAT GTCACTGTCA CTGTGGACCG   241 AGACCGCTTC ATGGATGAAT TCTTTGAACA GGTGGAAGAG ATCCGGGGCT TTATTGACAA   301 GATTGCCGAA AACGTGGAGG AGGTGAAGCG GAAACACAGC GCCATCCTGG CCTCCCCGAA   361 CCCCGATGAG AAGACAAAGG AGGAACTGGA GGAGCTCATG TCGGACATTA AGAAGACAGC   421 GAACAAAGTT CGCTCCAAGC TAAAGAGCAT TGAGCAGAGC ATCGAGCAGG AGGAAGGTCT   481 GAACCGCTCA TCAGCCGACC TGAGGATCCG GAAGACGCAG CACTCCACGC TGTCCCGAAA   541 GTTTGTGGAG GTCATGTCCG AGTACAACGC CACTCAGTCA GACTACCGAG AACGCTGCAA   601 AGGGCGCATC CAGAGGCAGC TGGAGATCAC CGGCCGGACC ACGACCAGTG AGGAATTGGA   661 AGACATGCTG GAGAGTGGGA ACCCTGCCAT CTTTGCCTCT GGGATCATCA TGGACTCCAG   721 CATCTCGAAG CAGGCCCTCA GTGAGATCGA GACCAGACAC AGTGAGATCA TCAAGCTGGA   781 GACCAGCATC CGGGAGCTGC ACGACATGTT CATGGACATG GCCATGCTGG TGGAGAGCCA   841 GGGGGAGATG ATTGACAGGA TCGAGTACAA TGTGGAGCAC GCCGTGGACT ACGTGGAGAG   901 GGCCGTGTCA GACACCAAGA AAGGCCGTCA AGTACCAGAG CAAGGCGCGC AGGAAGAAGA   961 TCATGATCAT CATTTGCTGT GTGATTCTGG GCATCATCAT CGCCTCCACC ATCGGGGGCA  1021 TCTTTGGATA GAATCACTAG TGAAATTCGC GGGCCGCCTG CAGGTCGACC ATAGCGGCCG  1081 CCTCTAGAGT GAGGGTCCCC CACCTGGGAC CATGGAGAGT ATCAAGTCTC CCAACGTGGG 1141 AGACAAAGAA ATCCCCTTGG ATTTTAAATA GT //
Primer: hGCSF-R Region: hGCSF+p(A), insert, FLAG tag Sequence file: RDB15166_A7Fmb.seq
>D04709A1_1_A7Fm_hGCSF-R_B01_04_ABI24.ab1     1 GGGGGGAAAT GAATCTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGAG CGGCCGCTAT    61 GGTCGACCTG CAGGCGGCCG CGAATTCACT AGTGATTCTA TCCAAAGATG CCCCCGATGG   121 TGGAGGCGAT GATGATGCCC AGAATCACAC AGCAAATGAT GATCATGATC TTCTTCCTGC   181 GCGCCTTGCT CTGGTACTTG ACGGCCTTCT TGGTGTCTGA CACGGCCCTC TCCACGTAGT   241 CCACGGCGTG CTCCACATTG TACTCGATCC TGTCAATCAT CTCCCCCTGG CTCTCCACCA   301 GCATGGCCAT GTCCATGAAC ATGTCGTGCA GCTCCCGGAT GCTGGTCTCC AGCTTGATGA   361 TCTCACTGTG TCTGGTCTCG ATCTCACTGA GGGCCTGCTT CGAGATGCTG GAGTCCATGA   421 TGATCCCAGA GGCAAAGATG GCAGGGTTCC CACTCTCCAG CATGTCTTCC AATTCCTCAC   481 TGGTCGTGGT CCGGCCGGTG ATCTCCAGCT GCCTCTGGAT GCGCCCTTTG CAGCGTTCTC   541 GGTAGTCTGA CTGAGTGGCG TTGTACTCGG ACATGACCTC CACAAACTTT CGGGACAGCG   601 TGGAGTGCTG CGTCTTCCGG ATCCTCAGGT CGGCTGATGA GCGGTTCAGA CCTTCCTCCT   661 GCTCGATGCT CTGCTCAATG CTCTTTAGCT TGGAGCGAAC TTTGTTCGCT GTCTTCTTAA   721 TGTCCGACAT GAGCTCCTCC AGTTCCTCCT TTGTCTTCTC ATCGGGGTTC GGGGAGGCCA   781 GGATGGCGCT GTGTTTCCGC TTCACCTCCT CCACGTTTTC GGCAATCTTG TCAATAAAGC   841 CCCGGATCTC TTCCACCTGT TCAAAGAATT CATCCATGAA GCGGTCTCGG TCCACAGTGA   901 CAGTGACATC GTCGTCGTCA TCGCTGTCCT TGGCCGTGCG GAGCTCCTGG GTTCGGTCCT   961 TCATAGATCC CTTGTCGTCA TCGTCCTTGT AGTCCATGGT GGAATCGAAT TCCCGCGGGC  1021 CGCTCTAGAG AATTCCTCAC GACACCTGAA ATGGGAAGAA AAAAAACTTT GACCACTGTC  1081 TGAGGCTTGA GAATGACCAG ATCCAACTCA AAAAGGCATT CAGGAGATAC ATCAGGTGCC 1141 AAGCTGGCCT AACTTCAGTC CTCAACCCCA ACTTCAAGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.11

GNP_filter3_RDBDEP_html_221010.pl