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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse Munc18-1

Expression vector of mouse Munc18-1 [Stxbp1 in NCBI].

Catalog number RDB15177
Resource name pEF-FLAG-mouse Munc18-1
Clone info. Expression vector of mouse Munc18-1 [Stxbp1 in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Stxbp1 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Stxbp1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. J. Biol. Chem. 278 (17): 15390-15396, 2003)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 278 (17): 15390-15396, 2003)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15177 pEF-FLAG-mouse Munc18-1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15177_A7Fmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF-1alpha pro, FLAG, insert 5'
          Sequence file: RDB15177_A7Fma.seq check
          >D04709B3_2_A7Fm_EF1a-C_F_D03_12_ABI24.ab1
              1 CCTGGGGAAT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG
             61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
            121 ATTCGATTCC ACCATGGACT ACAAGGACGA TGACGACAAG GGATCCATGG CCCCCATTGG
            181 CCTCAAGGCG GTGGTCGGAG AGAAGATCAT GCATGATGTG ATCAAGAAGG TGAAGAAGAA
            241 GGGCGAGTGG AAGGTGCTGG TGGTGGACCA GTTAAGCATG AGGATGCTGT CCTCCTGCTG
            301 CAAGATGACA GACATCATGA CCGAGGGCAT CACAATTGTG GAGGATATCA ACAAGCGCCG
            361 AGAGCCGCTC CCCAGCCTGG AGGCCGTGTA CCTCATCACC CCATCTGAGA AGTCTGTCCA
            421 CTCTCTGATC AGTGATTTTA AGGACCCGCC GACTGCTAAA TATCGGGCTG CGCATGTGTT
            481 CTTCACAGAC TCGTGTCCAG ATGCCCTATT TAACGAGCTG GTAAAATCCC GAGCAGCCAA
            541 AGTCATCAAG ACGCTGACGG AAATCAACAT TGCGTTTCTC CCCTATGAGT CCCAGGTGTA
            601 TTCCCTGGAC TCCGCTGACT CTTTCCAAAG CTTCTACAGC CCTCACAAGG CGCAGATGAA
            661 GAATCCGATA CTGGAACGCC TGGCAGAGCA GATCGCAACC CTGTGTGCCA CCCTGAAGGA
            721 GTATCCAGCT GTGCGGTATC GGGGGGAGTA CAAGGACAAT GCCTTGCTGG CTCAGCTGAT
            781 CCAGGACAAG CTGGATGCCT ATAAAGCCGA CGATCCAACA ATGGGGGAGG GTCCGACAAG
            841 GCACGGTCCC AGCTCCTGAT CCTGGATCGT GGCTTTGACC CCAGCTCCCC TGTGCTCCAT
            901 GAACTGACAT TCCAGGCTAT GAGTTACGAC CTGCTGCCTA TCGAAAATGA TGTTTACAAG
            961 TATGAGACCA GCGGCATTGG AGAGGCGCGG TGAAGGAGGT GCTACTGGAT GAGGACGATG
           1021 ACCTGTGGAT TGCGCTGCGA CAACAAGCAC ATCGCAGAGG TGTCCCAGGA GTCCACCCGG
           1081 GTCTCTGACG AACTTTTCTC TAGCAGAGGA ATGAACACTG CCGGCAGGAC ACATTGCGGT
          1141 ACTGATCCCA GATGCCTGGA AGAAAAGTGT CTCA
          //
          Primer: hGCSF-R
          Region: hGCSF+p(A), insert 3'
          Sequence file: RDB15177_A7Fmb.seq check
          >D04709B3_2_A7Fm_hGCSF-R_E03_15_ABI24.ab1
              1 GTGGGGACTG ATCTCTCAGG GTCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT
             61 CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTTAACT GCTTATTTCT TCATCTGTTT
            121 TATTCAGCTT CTTCAGCGTG TCCAGCAGTT TCTGTGGGGT GAGAATGTGC GTGGATCCTA
            181 TCAGCACTTC CCACTTGCCG TTGGCCTGGG TCACTTCGTA AGCACAGCGC ATCTCATTCA
            241 GGCTCACACC CCCAAGGATG AAAATAATGA GGCGGGGACC GCTGCGGTAC TCCCCGGGGG
            301 CCTTATTCTT GTGCCAATGT CCATAGCGGG CACTCACAGC AGTGGTGCTG AAGGACGCGG
            361 ACGAGCGGGT AGAGATGTAT GGGTAGTGCT TTGTATCCAG CTTGTCTTCG ATAGTGTCCT
            421 CCATAATGTC TTTAATGATC GGGGTCCATC GTGAGAGCTG GTAGGTCTGC TCACTGATAC
            481 GCTCCTTCCG CTCCGGTTTG CTTCGGCGGC GTAGTGTGGA ATCCGTGACG ATGGGCACGC
            541 CGAGGTGAGC CATGTTGGTG ATGATCTCGC TGTCCTCTGG GGGTATCTGA GCGTGCTGGA
            601 TGAGTTTGTT TAGGTTCTCC TCAGTGATAC CGTTCTTCAG GAAGATGTAG AGAAGGATGA
            661 TACGGATTTT GTCGTAAGTG CTGACGTTCG CATCCAGCAG GATGGGGACA ATGGCTCTCA
            721 TGGGGTCCTT GATTTTTTCC CCCTCAGCAT CTGTGCCCAT TGCCAGGTCC TGCTCCACGC
            781 GGCAGAGTTT GTCTACAGTG CCTTGGTAAT GCTTCATGCA GTCTTCAGCA AGGTGCAGGT
            841 GAGTCGAATA CTTGCTGAGC TCCTTCTGGT ACTGGGGCAT TTTCTTCAGC ATCTGGGACA
            901 GGTCCCGCAT GGTGGTCTTC TCGCCAGTGT TCATCCTCTT GCTAGAGGAA AAGTCCTTCA
            961 GAGACCGGGT GACTTCCTGG GACACCTCTG CGATGTGCTT GTGTCGCAGC GCAATCCACA
           1021 GGTCATCGTC CTCATCCAGT AGCACCTCCC TTCACCCGCG CCTCTCAATG CCGCTGGTCT
           1081 CATACTTTGT AAACATCATT TTTCGGATAG GCAGCAGTTC GTACTTCATA GCTGGATGGT
          1141 CAGTTCATGG AAGCCACAGG GGAAGCTGGG GGTTCCAAAG GTTCAAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl