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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-mouse FLAG-Munc13-1

Expression vector of mouse Munc13-1 [Unc13b in NCBI].

Catalog number RDB15180
Resource name pEF-mouse FLAG-Munc13-1
Clone info. Expression vector of mouse Munc13-1 [Unc13b in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Unc13b cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Unc13b
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Genes Cells 9(9): 831-842, 2004)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Genes Cells 9(9): 831-842, 2004)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15180 pEF-mouse FLAG-Munc13-1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuda, M., Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution? Genes Cells 9 (9): 831-842 (2004). PMID 15330860.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15180_A7Fmp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15180_A7Fma.seq check
          >D04709B6_2_A7Fm_EF1a-C_F_B04_04_ABI24.ab1
              1 CCTGGGATGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
             61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
            121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGTCGC TGCTCTGTGT
            181 GCGTGTTAAA AGAGCCAAAT TCCAGGGTTC ACCAGATAAA TTTAACACAT ATGTGACCCT
            241 GAAAGTCCAG AACGTGAAGA GCACAACTGT AGCAGTTCGT GGTGATCAGC CTTCCTGGGA
            301 GCAGGATTTC ATGTTTGAGA TCAGTCGTCT GGACCTGGGC CTAAGTGTGG AGGTGTGGAA
            361 CAAGGGACTG ATATGGGACA CAATGGTGGG CACCGTGTGG ATTGCACTGA AGACAATCCG
            421 TCAGTCGGAT GAGGAAGGGC CCGGGGAGTG GTCTACACTG GAGGCAGAGA CGTTAATGAA
            481 AGATGATGAG ATCTGTGGAA CTAAAAACCC AACTCCTCAT AAAATCTTGC TTGATACAAG
            541 ATTTGAGCTG CCTTTTGACA TCCCAGAGGA GGAAGCCAGA TATTGGACCT ACAAATTGGA
            601 GCAAATCAAT GCCTTGGCGG ATGATAATGA GTATTCTAGT CAAGAAGAAA GCCAGAGGAA
            661 GCCATTACCC ACTGCTGCCG CCCAGTGTTC CTTTGAAGAC CCTGATAGTG CCGTTGATGA
            721 CCGAGACAGT GACTACCGCA GTGAGACCAG CAATAGCGCC CCACCTCCTT ACCACACGAC
            781 TACCCAGCCC AACGCTTCTG TGCACCAGTT CCCTGTTCCT GTGCGGCTGC CACAGCAGCT
            841 CTTTCTTCAA GGCAGTTCCC ATGACTCTTG CAATGACTCT ATGCAGAGTT ACGACCTTGA
            901 TTATCCAGAG CGTCGGGCCC TCAGCCCTAC CAGCAGTAGT AGGTATGGCT CCTCCTGTAA
            961 TGTGAGTCAA GGAAGCTCTC TGCTGAGTGA ACTGGACCAG TATCATGAGC AAGATGATGA
           1021 TGGGTCGGGG AGAGGGACTC CATTTCATTC TAGTCATAGC TATGGGCAGC CTCCTCCAAG
           1081 ATGACCAGGC TGGGATTTGG AGACAAGGAG AAGCCCTTGT AGTGACATGT GAATTCAGTA
          1141 CAGAGCAATC GTTGCATTCC GAAAAGGCCA GGAT
          //
          Primer: hGCSF_R
          Sequence file: RDB15180_A7Fmb.seq check
          >D04709B6_2_A7Fm_hGCSF-R_C04_07_ABI24.ab1
              1 TGGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCTATGG
             61 TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTCAGC TCCCTTCCTC TGTGGATCGA
            121 GACTCTGATT TGAGCTTCAC GAACTCTCGG GCTACCTCGT CATTGCTCCT CTGAGACAGA
            181 ATCCGGAGAA TGGTCATGCC TGTCTCATCC ATATGGATCT TCCGGCCCAG TGGGCACCAG
            241 CAGGCACAGC TGCCCTTAGC TGCCACATCC CTCAGGGGCA TCACTGCCAG TCCTATCACA
            301 CGATCCTCCC GGGCAAAGCA GTAATCCTTC ACACAGATCT GCAATTCATA GGCCTCTGGA
            361 CCCTCTTCAT TTCCCAGGAG GAAGTGAAAC GTCTCGTTGT ACTTGGGAGT CCAGTTGTTA
            421 CTTTTAGACT TGGTTGTGAA CTTCCTCTTC TTATCACTTT GGTGTGGGCC AACCATGGTC
            481 ACTTCCACAA AAGGCCGGAA CATACCCGCT GTCTGCCACT TGAGGTCATT GGCTGCTACC
            541 ACTTTCACTG TGACCTTATG CTCCCCAGTG CCAGGATGTG TAAATAAGTC CACCTGAATA
            601 GAGACTTCTC CCACGGGGTC ATCCACACCA GCCCCCTGGG CAGTCTGTGA GCGCACAAAT
            661 GTCTTGATGA GAGTGTCTGT AGTCTGTGTA TACAGAGAGA GGGCATAACG CAGAGACTGC
            721 AGGTCTGGGC TCTTTTCCAG GAAGGTTTTC TTCAGACCAT TGCCTCCTGC ATGGAAGTAT
            781 TGCTTGATGG TGTCCAAGGC CAAGTCGAGG ACAGCACACT GCTTTGGAGT GAGATTCCGT
            841 GTTTCCTCCC GTACCATGTG GTCCTTGAGT TTGGAAAGCT GGCTCAGCTC CTTGGCAGCA
            901 GTTAAGATCA GCTGGGTGCC CGTCTGGTCA GTGAGTGGGG GCAGGACAAT AACCCGCTCC
            961 ATGGTGTTCA TCACCACCCG CCAGAGCTCC TTCAGGACCC GCTTCAGAAC TGTCTTCTCA
           1021 CACACAGTGG CAAACAGCGT GAGGTTGCCC ATCCAGGAAG TCCATGAGAG GTCGCAGCAC
           1081 ACTATTCTGC ATCCTGAGCC ACCGAAGTCC CTTGGCCGTA AGGAATGCAT CCTGTGCTCC
          1141 GAACTTGGAC CCAAGGAATG TTCA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Fukuda, M., Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution? Genes Cells 9 (9): 831-842 (2004). PMID 15330860.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl