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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG2-mCherry-C1-IFT122 (#RDB15191)

Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.

Clone info. Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAL009C12 (HGY083660).
Vector backbone pCAG2-mCherry-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name human IFT122 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15191 pCAG2-mCherry-C1-IFT122 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG2-mCherry-C1-IFT122 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15191).

Reference section:

Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15191_A7Fsp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mRFP1-inner1 (Pr0443)
Region: mCherry, insert 5'
Sequence file: RDB15191_A7Fsa.seq check
>D04646A2_A7Fs_2_mRFP1-inner1_E07_13_ABI24.ab1
    1 GAACGGCGTT ATGACGGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA
   61 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT
  121 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT
  181 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA
  241 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT
  301 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA
  361 CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
  421 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT
  481 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA
  541 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG
  601 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT
  661 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG
  721 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT
  781 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG
  841 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT
  901 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC
  961 ATATGGGTCC ATCTGCTGGA CCCTTTCAGA GAGGACGTAA TGACATCCTT GCTTGTTGCT
 1021 GACTGGGGAC AAGAAAGTTC GTCCTACAGC TGATGAAAAC AGATGGAAAA GGATCCGGCA
1081 CTGGACTTTG ACCTGCTGCA TCAGCTACTT ACTAAGGCGA GTACATTTGC CTGAG
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globinpA,insert 3'
Sequence file: RDB15191_A7Fsb.seq check
>D04646A2_A7Fs_2_pCAGGS_R1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNCNCNCT ATTGCNNTGN NAATAAATTT CCTTTATTAG CCAGAAGTCA
   61 GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCCCAGT
  121 GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA
  181 CCTGAGGAGT CTATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACT
  241 CATGGGCCAG GGTCATCCTT GCACCTGCGG CAGTAGGGGC AGCAGCCATG CTGAAGCACC
  301 AGCAACTCAT AGTCCTCAGA ATGGAACATC TGGAAGCAGG AGGGGCACAT GGTAATGGAG
  361 GCGTCAGGCA GCAGTGAGCG GAAGTATTGC CACCTCAGGG GTGGGGGCCA TCGCTTGATG
  421 AGGACATCCC GGCGGCTCAT GGAGCGCAGC ACCAGCCGGC TCACCACCAC TGGCACGAAC
  481 TCTGAGCCAC CTTGCTCAAA GCTCAGCTTA GCTGTGAACG GGTCCTCATC TCCGATGGAG
  541 TCCTTGGTCT CCACTAGCCG CAGAATCTGG GAGCTGTTGT TTGCAATCTC TAGCTGTCTG
  601 TCATCCCGCT TGGGTCTCAG CACCTCCAGG TCGATGAGGG AGATGGCTTC TTCATCAGTG
  661 ATCCCTTCCT CCAGGTAGAA CTCAACCAGG TGTAGCACGT CGTANGAAGA GGCGGANAAG
  721 ATGAAGGGCT GGCGGCAGTT GATGCAGACG TTGCCCAGGT TGTTGAGCAG CGGGTTGTTG
  781 GTGGAGCAGC GGTAGCACAA GGGCACCAAC TCCTCACTGT CGTGNAANGG CTTGGCGCGG
  841 ANGGTCAGGG TACCCAGCTC AATGNANTTT TNGAATCTGG CANGGNNGTN CANGCCACGC
  901 ANCTNGNCAT ANGCGNGNNN GGNCAGNNTG NNNNCNNCNA NGGCCTNNNN NNGCTNNNNN
  961 NANNNTGAAN ANTATTTTCN NNTNNNNNNN NNCNNNNGGG NNNNNNNTNN GNNNNNNNNN
1021 N
//
Primer: Red-N_R (Pr0329)
Region: mCherry, CAG pro
Sequence file: RDB15191_A7Fsc.seq check
>D04646A2_A7Fs_2_Red-N_R_E05_05_ABI08.ab1
    1 GACTTAGGGG GCACCTTTGA AGCGCATGAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG
   61 CCCTTGCTCA CCATGGTGGC GCTAGCATTT GCCAAAATGA TGAGACAGCA CAATAACCAG
  121 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTAG
  181 AGCCGCCGGT CACACGCCAG AAGCCGAACC CCGCCCTGCC CCGTCCCCCC CGAAGGCAGC
  241 CGTCCCCCCG CGGACAGCCC CGAGGCTGGA GAGGGAGAAG GGGACGGCGG CGCGGCGACG
  301 CACGAAGGCC CTCCCCGCCC ATTTCCTTCC TGCCGGCGCC GCACCGCTTC GCCCCGCGCC
  361 CGCTAGAGGG GGTGCGGCGG CGCCTCCCAG ATTTCGGCTC CGCACAGATT TGGGACAAAG
  421 GAAGTCCCTG CGCCCTCTCG CACGATTACC ATAAAAGGCA ATGGCTGCGG CTCGCCGCGC
  481 CTCGACAGCC GCCGGCGCTC CGGGGGCCGC CGCGCCCCTC CCCCGAGCCC TCCCCGGCCC
  541 GAGGCGGCCC CGCCCCGCCC GGCACCCCCA CCTGCCGCCA CCCCCCGCCC GGCACGGCGA
  601 GCCCCGCGCC ACGCCCCGTA CGGAGCCCCG CACCCGAAGC CGGGCCGTGC TCAGCAACTC
661 GGGGAGGGGG GTGCAGGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]