Please review the QC test results indicated by icon below as well as clone information before placing your order.

tPA-mK-GO-N1

Tissue-type plasminogen activator (tPA)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15246_B1Kpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' (tPA 5') Sequence file: RDB15246_B1Kpa.seq
>D04498F4_B1Kp_2_CMV_Forward_A06_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GACTCAGATC   121 TCGAGCTCAA GCTTCGAATT CTGCAGTCGA CATGAAGGGA GAGCTGTTGT GCGTCCTGCT   181 GCTTTGTGGA GTGGCGTTCA CGTTGCCTGA CCAGGGAATA CACAGGAGGT TCAAAAGAGG   241 AGCTCGGTCC TACAGAGCGA CCTGCAGAGA TGAACAGACT CAGACAACTT ACCAACAGCA   301 TCAGTCATGG CTACGTCCCA TGCTCAGAGG CAATCGGGTG GAATACTGCC GGTGCAACAG   361 CGGCCTGGCA CAGTGCCACT CGGTGCCCGT CCGAAGTTGC AGTGAACCGA GATGCTTCAA   421 TGGGGGTACG TGTCAGCAGG CCCTGTATTT CTCTGACTTC GTCTGCCAGT GCCCTGACGG   481 ATTTGTTGGG AAACGCTGTG ATATAGATAC CAGAGCAACC TGCTTCGAGG GCCAGGGCAT   541 CACCTACAGA GGCACATGGA GCACAGCAGA AAATGGGGCT GAATGCATCA ACTGGAATAG   601 CAGTGCCCTG TCCCAGAAGC CCTACAGTGC AAGGAGGCCA AATGCCATCA AGCTGGGCCT   661 TGGGAACCAC AATTACTGCA GAAACCCAGA CCGAGACGTG AGGCCCTGGT GCTATGTCTT   721 TAAGGCAGGG AAGTATACCA CGGAGTTCTG CAGCACGCCG GCTTGCCCTA ANGGANCAAC   781 TGAGGACTGC TATGTTGGAA AAGGTGTGAC TTACCGTGGC ACCCACNGCT TTACCACATC   841 CAAGGCCTCC TGCCTCCCAT GGAATTCCAT GATCCTGATA GGNNAGACTT ACACAGCGNG   901 NANGGNCNAC TCCCANGCAC TTGGCCNNNN NAGANNNAAT TATTGCCGGN NNCNNNATGG   961 GGNNGNCAAA CCTTGGNNNC NNNNGATGNN NNNNNAANNC TGANNTGGGA ANATTGCNNN 1021 NTGNCCCNNN NNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' (mK-GO 3') Sequence file: RDB15246_B1Kpb.seq
>D04498F4_B1Kp_2_SV40polyA_R2_B06_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNNNNNN ATTGCNTTCA TTTTATGTTT    61 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT   121 ATGGCTGATT ATGATCTAGA GTCGCGGCCG CTTAGGAATG AGCTACTGCA TCTTCTACCA   181 GCTCAGTAAT GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCCGATGTAA TGGTCTCCTG   241 GCATTTCAAG AATCTCTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT TTGTGATTGC   301 CGCCTCCTTC AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG TCGCTGGCAG   361 TAATTTTCTC GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA GGACCATCGG   421 CAGGAAAGTT AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT CTAAGGCTTA   481 TATGCGCACT GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT TCCCATGACA   541 GGCCTTCAGG AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT TTAGTAAATA   601 CTCTGTGGCC GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAAGGC ATTGGCCCGC   661 CCTCGGCCAT TGTGACGCGT AGTGTCATCT CTTGATGTCC CTCGTAAGGT CTGCCTGTGC   721 CTTCACCTTC AATTGTGAAC TCATGCCCAT TGACGGAGCC GTCCATGTAG TACCTCATCT   781 TCATCTCTGG TTTANTCACA CTCNCCATGA NCGGTGGATC CCGGGNCCGC GGTTGCTTCA   841 TGTTGTCTTG NATCCANTTC ANGTAATTAG TGACCTTTGT GTATATCCCT GGCACGNCCT   901 TNTGCCCACA GCCNANGNCC CAGCTGATGA TGNCCANNNA AGTCATNCGN NTNNCGATCA   961 TGCNCANNNN NNGGNCNCCT GANNNANCCT GGCANNCGTC ANNNNNGTCN TNGNNTNNCN 1021 NCNNNNNNCN GGNNNNNCTN CTGNNNNNNN NNN //
Primer: mKO2-N_R (Pr0494) Region: insert mid (mK-GO 5', tPA 3') Sequence file: RDB15246_B1Kpc.seq
>D04498F4_B1Kp_2_mKO2-N_R_D06_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNNNNNNNGT CNNTGTNNNN NNTCATCTTC    61 ATCTCTGGTT TAATCACACT CACCATGACC GGTGGATCCC GGGCCCGCGG TTGCTTCATG   121 TTGTCTTGGA TCCAGTTCAG GTAATTAGTG ACCTTTGTGT ATATCCCTGG CACGTCCTTC   181 TGCCCACAGC CGAGGCCCCA GCTGATGATG CCCAGTAAAG TCATCCGCTT ATCGATCATG   241 CACACCAGAG GGCCTCCTGA GTCACCCTGG CACGCGTCAT GGACGTCTTG GTTGCCCCCA   301 GTTCGGGTGT CTCCTGCACA CAGCATGTTG CTCGTGATGG TTTTGTTAAA CAGATGCTGT   361 GAGGTACAGC GGCTGGACGG ATACAGTCTG ACGTGAGCCT CCTTCAGCCG GTCAGAGAAG   421 AAAGGAGAGG ATGCCTCATG CTTGCCGTAG CCAGAAAGCT CACACTCTGT CCAGTCAGGA   481 AGCTGTACGT CGGGGTCAGG GAGGCAGGCA GTGCCGACAG AACTGCTCTC CTGGGCACAC   541 TGACTGGAAT CTGACCTCAG CTGCAGTAAT GCGATGTCAT TGTCATAAGT GTCGTCATCA   601 AATTCCTTAT GGACTATGTA CTTTTCGATC TCGAATGTCT GCTCCTCCTC TCCAGGGACC   661 ACTCTGTATG TTCTGCCCAA GACCACTTTA AGATGATGGG GTGGAAACCT NNNNNNNAAA   721 GCAGTGGGCG GCAGATAGCA CCCAGCANGA ACTGATCAGC ACCCCTCNNN NCANGANNCT   781 CTCTNCNNGG ANACCTCTTG TTCNTGACAA AGATGGCGNN CTGCCNNNGN TGTGANGTGA   841 NGTCTGTGAA GAGTCCNNCN TTTAANTNCG AAACNGANGC TGTTTGTATT GNCCTCNNNC   901 CGCNNNGGAN CATGGGGNAC NTGTCGCAAT NNTTNCCATG TNNGCTTTNN GNCNNTTNNN   961 NACGNGGCAC CAAGGTTNNN NNNNNCCCCN TNTGGNNNNC NNNNANNANT TGNGTCTNNC 1021 CNNNNNNANN NNNNNGNNAN NTNGNCCNNC NNCGCNNNNN NANNNNNTNN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB15246_B1Kpd.seq
>D04498F4_B1Kp_2_SV40pro_ori_F_C09_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCTCGGCC TCTGANCTAT TCCNGAAGTA GTGAGGAGGC    61 TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG   121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT   181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC   241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT   301 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT   361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA   421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT   481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG   541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA   601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA   661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA   721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA   781 CATAGCGTTG GCTACCCGTG ATATTGCTGA ANAGCTTGGC GGCGAATGGG CTGANCGCTT   841 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCANCGC ATCNCCTTCT ATCGCCTTCN   901 TGANGAGTTC TTCTGANCNG GANTCTGGGG GTTCGAAATG ACCGACNNAG CNNCNCCCNA   961 CCNGNCNTCN CNANNTTTCN ANTNNNNNGN CNNCNNTCNA TNNNNNNNGG GCTTCNNNNN 1021 NNNTTNNNNG NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl