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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GEPRA-B

Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.

Catalog number RDB15249
Resource name GEPRA-B
Clone info. Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.
Vector backbone pCS2+ (plasmid)
Gene/insert name mouse RARbeta cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
mouse RARbeta Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15249 GEPRA-B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Sensor and Visualization (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15249_A7H9p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCMV-IE94_F ()
          Sequence file: RDB15249_A7H9a.seq check
          >D04498F7_2_A7H9_pCMV-IE94_F_B05_05_ABI24.ab1
              1 AAGGGCGGGT AGCGTGCCTA ATGGGAGGTC TATATAAGCA ATGCTCGTTT AGGGAACCGC
             61 CATTCTGCCT GGGGACGTCG GAGCAAGCTT GATTTAGGTG ACACTATAGA ATACAAGCTA
            121 CTTGTTCTTT TTGCAGGATC CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
            181 GCCCATCCTG GTCGAGCTGC AGGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA
            241 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
            301 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGAGC TGGGGCGTGC AGTGCTTCAG
            361 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGTCATGC CCGAAGGCTA
            421 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT
            481 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA
            541 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC ATCAGCCACA ACGTCTATAT
            601 CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGT
            661 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
            721 CGTGAACCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA
            781 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCCGCA TGCATGAGAG
            841 CTATGAGATG ACAGCGGAGC TAGACGACCT CACTGAGAAG ATCCGGAAAG CCCACCAGGA
            901 AACCTTTCCC TCACTCTGCC AGCTGGGTAA ATACACCACG AATTCCAGCG CTGACCACCG
            961 GGTCCGATTG GACTTGGGCC CTCTGGGACA AATTCAGTGA GCTGGGCCAC CAAGTGCATT
           1021 TATTTAGATC GTGGAGTTTC GTAGCGTCTG CCGGGCTTTC ACAGGTCTGA CATCGCAGAC
          1081 AGATCACCTT GCTCAAGGCG GCTGCCTTGC ATATTCTTGG ATTTCCTCAG AATTTGTA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB15249_A7H9b.seq check
          >D04498F7_2_A7H9_pAxCALNL_F1_A05_02_ABI24.ab1
              1 ACAAGCATCT TATCATGTCT GGATCTACGT AATACGACTC ACTATAGTTC TAGAGGCTCG
             61 AGTTACTTGT ACAGCTCGTT CATGCCTTCA GTGATCCCGG CGGCGGTCAG GAACTCCAGC
            121 AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGAACA GGGCGGACTG GTAGCTCAGG
            181 TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG
            241 TCGGCGAGCT GCACGCCGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG
            301 CCGTTCTTCT GCTTGTCGGC GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC
            361 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC
            421 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG
            481 AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC
            541 TGCTTCATGT GGTCGGGGTA GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG
            601 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA GCAGCTTCAG GGTCAGCTTG
            661 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
            721 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
            781 ATGAGCTCTT CATGTCCTTC AGAATTCTCC AGCATTTCCT GAATGAGAGG TGGCATTGAT
            841 CCAGGAATTT CCATTTTCAA GGTAATTACA CGTTCGGCAC CTTTCGCGCT GATGCTGCGG
            901 AGATCTGTGA TTTTCATTAA GATCTTTGGA AACATGTGAG GCTTGCTGGG TCGTCGTTTT
            961 CTAATGTAAA TCTTTAGTGC TTCCAGCAGT GGCTTCTTGG AGCTTGTCTA CTTTTGTGGT
           1021 TCTCAGGTCC TGGCGGGTCT CACAGATTAA ACAGATGGGC ACTGAGAGGC TGCTTTCTGT
           1081 GTCATCATTT CGAAAGGCAG AGCTGTTGCC AAAGTGACAC AGGGTTCAGT CAGGAAGGAT
          1141 CT
          //
          Primer: RARbeta_LBD
          Sequence file: RDB15249_A7H9c.seq check
          >D04498F7_2_A7H9_RARbeta_LBD_C05_08_ABI24.ab1
              1 CGGAGGAAGA GGTGGCATTG ATCCAGGATT TCCATTTTCA AGGTAATTAC ACGTTCGGCA
             61 CCTTTCGCGC TGATGCTGCG GAGATCTGTG ATTTTCATTA AGATCTTTGG AAACATGTGA
            121 GGCTTGCTGG GTCGTCGTTT TCTAATGTAA ATCTTTAGTG CTTCCAGCAG TGGTTCTTGG
            181 AGCTTGTCTA CTTTTGTTGG TTCCTCAAGG TCCTGGCGGT CTCCACAGAT TAAACAGATG
            241 GCACTGAGAA GGCCTGTTTC TGTGTCATCC ATTTCCAAAG GCAGGAGCTG GTTGGCAAAG
            301 GTGAACACAA GGTCAGTCAG AGGACCGAAG CCAGCATTGT GCATCTGAGT TCGATTTAGT
            361 GTAAGGCCAT CAGAGAAAGT CATGGTGTCT TGCTCTGGGG TATACCTGGT ACAAATTCTG
            421 AGAATCAAGA TATCCAAGCA GGCGGCTTTG AGCAGGGTGA TCTGGTCTGC GATGGTCAGA
            481 CCTGTGAAGC CCGGCAGACG CTTGGCGAAC TCCACGATCT TAATAATGCA CTTGGTGGCC
            541 AGCTCACTGA ATTTGTCCCA GAGGCCCAAG TCCAATCGGA CCCGGTGGTC AGCGCTGGAA
            601 TTCGTGGTGT ATTTACCCAG CTGGCAGAGT GAGGGAAAGG TTTCCTGGTG GGCTTTCCGG
            661 ATCTTCTCAG TGAGGTCGTC TAGCTCCGCT GTCATCTCAT AGCTCTCATG CATGCGGGCG
            721 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
            781 GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGGTTCA CGGGGCCGTC GCCGATGGGG
            841 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCACGATGTT GTGGCGGATC
            901 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
            961 ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTTGCCC GTCCTCCTTG AAGTCGATGC
           1021 CCTTTCAGCT CGATGCGATC ACAGGGTGTC GCCCTCGACC TCAACTCGGC GCGGGTCTTG
           1081 TAGCTGCGTT CGTTCATGGA GAGATGCAGC GCTCCTGACG ATAGCTCATC GGGTCAAGTG
          1141 AAACG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.
          review Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl