Please review the QC test results indicated by icon below as well as clone information before placing your order.

GEPRA-G/ pT2KXIGdeltain

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15253_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2 Sequence file: RDB15253_A7H9a.seq
>D04498G2-1_A7H9_EF1a-C_F2_G10_19_ABI24.ab1     1 GTTGGGGTGT AAGGCCCGGT TCGCTCTCTT CCTCACCGGG TCTGCGGCGA GTTCTAGCTG    61 AAGCTTCCTG CAGGTCGACC GATCCTGAGA ACTTCAGGGT GAGTTTGGGG ACCCTTGATT   121 GTTCTTTCTT TTTCGCTATT GTAAAATTCA TGTTATATGG AGGGGGCAAA GTTTTCAGGG   181 TGTTGTTTAG AATGGGAAGA TGTCCCTTGT ATCACCATGG ACCCTCATGA TAATTTTGTT   241 TCTTTCACTT TCTACTCTGT TGACAACCAT TGTCTCCTCT TATTTTCTTT TCATTTTCTG   301 TAACTTTTTC GTTAAACTTT AGCTTGCATT TGTAACGAAT TTTTAAATTC ACTTTTGTTT   361 ATTTGTCAGA TTGTAAGTAC TTTCTCTAAT CACTTTTTTT TCAAGGCAAT CAGGGTATAT   421 TATATTGTAC TTCAGCACAG TTTTAGAGAA CAATTGTTAT AATTAAATGA TAAGGTAGAA   481 TATTTCTGCA TATAAATTCT GGCTGGCGTG GAAATATTCT TATTGGTAGA AACAACTACA   541 CCCTGGTCAT CATCCTGCCT TTCTCTTTAT GGTTACAATG ATATACACTG TTTGAGATGA   601 GGATAAAATA CTCTGAGTCC AAACCGGGCC CCTCTGCTAA CCATGTTCAT GCCTTCTTCT   661 CTTTCCTACA GCTCCTGGGC AACGTGCTGG TTGTTGTGCT GTCTCATCAT TTTGGCAAAG   721 AATTCCTCGA CGGATCCCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC   781 ATCCTGGTCG AGCTGCAGGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC   841 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG   901 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGAGCTGGG GCGTGCAGTG CTTCAGCCGC   961 TACCCCGACC ACATGAAAGC AGCACGACTT TCTTCAAGTC CGTCATGCCC GAATGCTACG  1021 TCCAGGAGCG CACCAATCTT CTTCAGGACG ACGGCACTAC AAGACCGGCG CGAGTGAGGT 1081 TCGAAGGCGA CACCTGGTGG ATCGCAATCG AGCTGGAAAG GGCCATCCGA ACCTT //
Primer: pAxCALNL_F1 Sequence file: RDB15253_A7H9b.seq
>D04498G2_1_A7H9_pAxCALNLF1_D01_04_ABI08.ab1     1 GAAAGGGTAT CTTATCATGT CTGGATCATC ATCGATGAAT TAATTCGAGC TCCACCGCGG    61 TGGCGGCCGC CAGTGTGATG GATATCTGCA GAATTCTTAG TCCTCGATGT TGTGGCGGAT   121 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT   181 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC   241 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT   301 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC   361 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC   421 GTAGCCCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAG   481 CAGCTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA   541 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG   601 CTCCTCGCCC TTGCTCACCA TGCCACCGGT ACCGCCCTTG TACAGCTCGT TCATGCCTTC   661 AGTGATCCCG GCGGCGGTCA GGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG   721 GTCTTTGAAC AGGGCGGACT GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC   781 GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CCATGAGCTC   841 CTCAAACATC TCCGGGTTCT CCAGCATCTC TCGGATCAGG GGTGGCATCG GGCCTGGAAT   901 CTCCATCTTC AGGGTTATAG CCCTTTCTGC TCCCTTAGTG CTGATGCCCC GGAGGTCGGG   961 TGATTTTCAT CAGCATCCTT GGGAACATGT AGGGTTGGCT GGGTCTCCGG TCGCCGGGCA  1021 ATAGAGCCCT CAGGCTTTCC AAGCAGGGGC CTCCTGCAGC TTGTCATCTT CTCGGGCTTC  1081 TTCAGGTCCA TCGGTCTTCC ACAGAATTGA GCCAGATAGC ACTTAGTTAG CTCCAGTCTC 1141 GTGATCATTC CATTTCTTCC AAGCG //
Primer: RARgamma_LBD Sequence file: RDB15253_A7H9c.seq
>D04498G2_1_A7H9_RARgamma_LBD_F01_06_ABI08.ab1     1 CTGAATAGGG GGTGGCATCG GGGGCCTGGG AATCTCCATC TTCAGGGTTA TAGCCCTTTC    61 TGCTCCCTTA GTGCTGATGC CCCGGAGGTC GGTGATTTTC ATCAGCATCC TTGGGAACAT   121 GTAGGGTTGG CTGGGTCTCC GTCGCCGGGC ATAGAGCCTC AGGGCTTCCA GCAGGGGCTC   181 CTGCAGCTTG TCCACCTTCT CGGGCTCTTC CAGGTCCATT CGGTCTCCAC AGATGAGGCA   241 GATAGCACTA AGTAGCCCAG TCTCGGTGTC ATCCATCTCC AGGGGCAGCA GCTGCCCGGC   301 AAAGGCAAAG ACGAGGTCTG TAAGGGGCCC AAAGCCAGCA TTGTGCATCT GGGTTCGGTT   361 CAGGGTCAGC CCATCCGAGA ATGTCATAGT GTCCTGCTCT GGGGTATACC TTGTACAGAT   421 CCGCAGCATT AGGATGTCCA GACAAGCAGC CTTGAGCAGC GTGATCTGGT CGGCAATGCT   481 GAGCCCTGTA AAACCAGGCA GCCGCTTCGC AAACTCCACA ATCTTGATGA TGCATTTGGT   541 GGCCAGCTCG CTGAACTTGT CCCACAGCCC CAGGTCCAGC TGCACCCGGT GATCTGCACT   601 GGAGTTCGTG GTGTACTTGC CCAGCTGGCA GAGTGAGGGA AAAGTCTCCT GGTGGGCTTT   661 GCTGACCTTG GTGATGAGTT CCTCTAACTG TGGACTCAGT TCATAGCTGT CATGCATGCG   721 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT   781 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGG TTCACGGGGC CGTCGCCGAT   841 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCACGA TGTTGTGGCG   901 GATCTTGAAG TTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT   961 GGGCTGATGT AGTTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGGTCC TTCCTTTGAA  1021 AGTCGATGCC CTTTCAGCTC GATGCGGGTT CAACCAGGGT GTTCGTCCTT CGAACTTTCA  1081 ACCTTCGGGC GCGGGTCTTT GTAGTTGGCC GTCGATCTTG AAGAGGATGG TTGGCGCTCT 1141 GACGTAAGCC CTTCGGGCAT GAACGGAACT TTTGGAAAGG A //
Primer: EF1a-N_R2 Sequence file: RDB15253_A7H9d.seq
>D04498G2-1_A7H9_EF1a-N_R2_A11_02_ABI24.ab1     1 GTGGCGGGGC AAGATTGCAG TTTGATTTGC ATTGGAAGGG TCGCTGGCTT TTGTGTTACA    61 CGCCCCTTAT TTGTGCTTGA TTAGATGATC CCCCTGCTCG AGGGCCCATC TGGCCTGTGT   121 TTCAGACACC AGGGAGTCTC TGCTCACGTT TCCTGCTATT TGCAGCCTCT CTATCAAGAC   181 TAATACACCT CTTCCCGCAT CGGCTGCCTG TGAGAGGCTT TTCAGCACTG CAGGATTGCT   241 TTTCAGCCCC AAAAGAGCTA GGCTTGACAC TAACAATTTT GAGAATCAGC TTCTACTGAA   301 GTTAAATCTG AGGTTTTACA ACTTTGAGTA GCGTGTACTG GCATTAGATT GTCTGTCTTA   361 TAGTTTGATA ATTAAATACA AACAGTTCTA AAGCAGGATA AAACCTTGTA TGCATTTCAT   421 TTAATGTTTT TTGAGATTAA AAGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA   481 CTTTTACTTC CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA   541 GTAAGATTCT AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT   601 ACTTTCACTT GAGTAAAATT TTTGAGTACT TTTTACACCT CTGTCAAGAA CTCCTGGACA   661 AACCTCTGAC CTGTGTGGAA CAGAGTGGAT ATGGGTGTCT GAACAGATAT TCACGTCTTT   721 TGCAGATCAG AGGGCATTTC TGGTGTTGTG CAATGACCTG GGTCCAACAG GTAGGCGTAC   781 TCGTATCCAA GAGCATTGTT GGACAGGAGG TAGTCTCCAT TCCTATAAAG AGGAAGGAAT   841 GGCACCATGT AGTAGCCGTC ATTGTGGCCA ATTGGTGCAT TGGTACGTGG GTAGATGGAC   901 CGGGGAGGCT GATGAGTTCT TAGCCAGCGC TCAAAGATGC TAGAAATGAT GTAAGAAACA   961 AATATGCAGA TGAAAGTTTA AGATAAGATA AAAAAATTTA AACAATAATG CATTTATGGT  1021 TTTGTGTTTC TATTCCACCA ATAATAACCT CACGCAGTAA AGCCACCAGA TATTTTTGGT 1081 TCGTTGGCTC TGCCAGGCAC GAAACCTCTC TTAGC //
Primer: pT2KXIG#1 Sequence file: RDB15253_A7H9e.seq
>D04498G2_1_A7H9_pT2KXIG#1_E01_05_ABI08.ab1     1 ACGCGCAAAT AACCCTCACT AAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC    61 GCTCTAGAAC TAGTGGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA ACCAGAGGTG   121 TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT   181 ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA   241 GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT   301 TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT TGCGCTGATG   361 CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC ATTATATGAA   421 ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC TATTACCACA   481 ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG TGGAAGAAAA   541 TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT CAGCCACAGG   601 ATCAAGAGCA CCCGTGGCCG TATCTTCGCA GATCTGATCT AGAGGATCAT AATCAGCCAT   661 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG   721 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC   781 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT   841 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCATCAT CGATGAATTA   901 ATTCGAGCTC CACCGCGGTG GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTAGTC   961 CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTTGATGCC CGTTCTTCTG CTTGTCGGCG  1021 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCCAG CTGTGCCCCA GGATGTTGCC  1081 GTCCTCCTTG GAAGTCGATG CCTTCAGCTC GGATGCCGAT CACAGTGTCG CCTCGAACTT 1141 CACTTCGACG CGGGGTCTTT GGTAGAATGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl