Please review the QC test results indicated by icon below as well as clone information before placing your order.

Dronpa2/pRSETB

Plasmid clone of Dronpa2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15257_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15257_A7H9a.seq
>15257_B0L4F_2_lacIpro-B_F_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNCGNNNC GNNTCNGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA    61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG GATCTCGATC CCGCGAAATT   121 AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT   181 TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG   241 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCGAT   301 GAGTGTGATT AAACCAGACA TGAAGATCAA GCTGCGTATG GAAGGCGCTG TAAATGGACA   361 CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG GAAGCCTTTC GAGGGAAAAC AGAGTATGGA   421 CCTTAAAGTC AAAGAAGGCG GACCTCTGCC TTTCGCCTAT GACATCTTGA CAACTGTGTT   481 CTGTTACGGC AACAGGGTAT TCGCCAAATA CCCAGAAAAT ATAGTAGACT ATTTCAAGCA   541 GTCGTTTCCT GAGGGCTACT CTTGGGAACG AAGCATGAAT TACGAAGACG GGGGCATTTG   601 TAACGCGACA AACGACATAA CCCTCCATGG TGACTGTTAT ATCTATGAAA TTCGATTTGA   661 TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT TATGCAGAAG AGGACTGTGA AATGGGAGCC   721 ATCCACTGAG AAATTGTATG TGCGTGATGG AGTGCTGAAN GGTGATGTTA ACACGGCTCT   781 GTCGCTTGAA NGANNTGGNC ATTACCGATG TGTCTTCNAA ACTACTTATA AAGCTAAGAN   841 GGTTGTCCAG TTGCCAGANT ATCACTTTGT GGACCACCAC ATTGAGATTA AAAGCCACGA   901 CAAAGANTAC NGTANTGNTN ATCTGCNTGA NCACGCCNAA NCGCATTCTG ANCTGNCNAN   961 GNNNNNCNAG TAANNNNTCN AANCTNGNTN CNNNTGNTNN CNAAGCCNNA ANNGNNNNNN 1021 NNNN //
Primer: T7ter_R1 (Pr0798) Region: insert, Xpress tag, T7 tag, 6xHis Sequence file: RDB15257_A7H9b.seq
>15257_B0L4F_2_T7ter_R1_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT    61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGGCCTG CCTCGGCAGC   121 TCAGAATGCG CTTCGGCGTG CTCATGCAGA TTAACATTAC TGTAATCTTT GTCGTGGCTT   181 TTAATCTCAA TGTGGTGGTC CACAAAGTGA TAGTCTGGCA ACTGGACAAC CCTCTTAGCT   241 TTATAAGTAG TTTTGAAGAC ACATCGGTAA TGGCCACCTC CTTCAAGCGA CAGAGCCGTG   301 TTAACATCAC CCTTCAGCAC TCCATCACGC ACATACAATT TCTCAGTGGA TGGCTCCCAT   361 TTCACAGTCC TCTTCTGCAT AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA   421 ATTTCATAGA TATAACAGTC ACCATGGAGG GTTATGTCGT TTGTCGCGTT ACAAATGCCC   481 CCGTCTTCGT AATTCATGCT TCGTTCCCAA GAGTAGCCCT CAGGAAACGA CTGCTTGAAA   541 TAGTCTACTA TATTTTCTGG GTATTTGGCG AATACCCTGT TGCCGTAACA GAACACAGTT   601 GTCAAGATGT CATAGGCGAA AGGCAGAGGT CCGCCTTCTT TGACTTTAAG GTCCATACTC   661 TGTTTTCCCT CGAAAGGCTT CCCAAGGCCA ACTCCTTCAA TCGCGAACGG GTGTCCATTT   721 ACAGCGCCTT CCATACGCAG CTTGATCTTC ATGTCTGGTT TAATCACACT CATCGGATCC   781 TTATCGTCAT CGTCGTACAG ATCCCGACCC ATTTGCTGTC CACCAGTCAT GCTAGCCATA   841 CCATGATGAT GATGATGANG AGAACCCCGC ATATGTATAT CTCCTTCTTA AAGTTAAACA   901 AAATTATTTC TAGAGGGAAA NCGTTGTGGN CTCCCNATAG TGAGNCGNAN TAATTTCGCG   961 GGNNNNANNT CCTGCNTTAA TGAATCGNNC NNCGCGCGGG GANANGNGGN TNGNGNATTG 1021 GGNNNTNNNN NNNNTNNNNN GNNNANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl