RDB15262 - Mermaid

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Karasawa S, Okamura Y, Miyawaki A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Karasawa S, Okamura Y, Miyawaki A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15262	Mermaid	DNA solution

original	Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396.

Featured content	Sensor and Visualization (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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Primer: pCMV-IE94_F Sequence file: RDB15262_A7H9a.seq
>D04498H2_2_A7H9_pCMV-IE94_F_A01_01_ABI08.ab1 1 GTGGGCGGGG AAGCGGTGCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAACCG 61 CCATTCTGCC TGGGGACGTC GGAGCTAGCT TGATTTAGGT GACACTATAG AATACAAGCT 121 TATGGAGGGA TTCGACGGTT CAGATTTTAG TCCTCCAGCT GATTTAGTTG GCGTTGACGG 181 TGCAGTCATG CGGAACGTCG TTGACGTCAC GATAAATGGT GACGTCACTG CTCCGCCGAA 241 AGCTGCGCCA AGAAAAAGTG AATCGGTAAA GAAAGTTCAT TGGAATGATG TAGACCAAGG 301 ACCGAGTGAA AAACCAGAGA CAAGACAGGA GGAACGAATA GATATACCCG AGATTTCAGG 361 TCTATGGTGG GGCGAGAATG AACATGGAGT GGACGATGGG AGAATGGAGA TACCTACTAC 421 TGGTGTAGGT CGCGTCCAGT TTCGTGTCCG AGCAGTGATT GATCATCTAG GGATGCGAGT 481 CTTTGGAGTC TTCCTAATTT TCTTGGACAT CATCCTCATG ATCATTGATC TCAGTCTTCC 541 AGGAAAAAGT GAATCTTCAC AATCCTTTTA TGACGGGATG GCTTTGGCTC TTTCTTGTTA 601 TTTCATGCTG GATTTAGGAT TAAGGATATT TGCCTACGGG CCCAAGAATT TCTTCACCAA 661 CCCCTGGGAG GTTGCTGATG GTTTGATTAT CGTTGTCACA TTCGTCGTCA CGATATTTTA 721 CACTGTGTTA GATGAATACG TGCAAGAAAC AGGAGCCGAT GGTTTGGGGC AATTGGTTGT 781 GTTGGCCCGT TTGCTGCGTG TGGTTCGATT AGCAAGAATA TTTTATTCCC ACCAACAAAT 841 GAAGGCTTCA AGCAGAAGAA CAATATCAGG GGATCCCATG AGTGTGATTA AAGAGGAAAT 901 GAAGATCAAG CTGCATATGG AAGGAAATGT AAACGGTCAT GCATTTGTGA TTGAAAGGAG 961 ATGGGAAAAG GGAAAGCCCT TACGATGGGA CACAGACTTT TAAACCCTGA CAGTGAAAGA 1021 AGCCGCAACC TCTCCCTTTT TCTTTACGAC CATCTGACCA ATGCGTTCCA GTTACCGAAA 1081 TAGAGCAATT CACTAAATAT TCCAGGACCG G //
Primer: mKO2-N_R Sequence file: RDB15262_A7H9b.seq
>D04498H2_2_A7H9_mKO2-N_R_B04_02_ABI08.ab1 1 GAATCTCATT GTGACTCATG CCCATTGACG GAGCCGTCCA TGTAGTACCT CATCTTCATC 61 TCTGGTTTAA TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGACTTGG CAGCAAAGAA 121 TTGCGAGCAA CCGCATTCTC ATACAGCTTG ACTTTGCTGT AATCCCTGTC ATGGCTCAAG 181 ATCTCGATAC GATGGTCGAT CTTGTGGGCA TCTGGCAACT GCACAACCTT TTTCGCTTTG 241 TAGGAAGTTT TGAAGTCACA TCGGTGATGG CCACCTCCTT CAAGCAACAG AGCCATGTTA 301 ACATCACCCA TCAGAAACCC ATCACGCACG TACATCATCT CAGTGGATGG TTCCCACTTC 361 AAAGTTTTCT TCTGCATAAC TGGACCATTG GGGGGAAAGT TCTTGCCATC AAACCGAATT 421 TTATAGATAA AGCAGTCGCC TTCCATGCTG ATCTCGCTTC TCACGTTGCA AATGGCGTTG 481 TCTTCATAAC TCATGGTTCT TTCCCATGAA TACCCCTCGG GAAACGTCTG CTTGAAATAG 541 TCTGGTATAT CGGCTGGATA TTTAGTGAAT GCTCTATTTC CGTACTGGAA CGCATTTGTC 601 AAGATGTCGT AAGAAAAAGG GAGAGGTGCG CCTTCTTTCA CTGTCAGGTT TAAAGTCTGT 661 GTCCCATCGT AAGGCTTTCC TTTTCCATCT CCTTCAATCA CAAATGCATG ACCGTTTACA 721 TTTCCTTCCA TATGCAGCTT GATCTTCATT TCCTCTTTAA TCACACTCAT GGGATCCCCT 781 GATATTGTTC TTCTGCTTGA AGCCTTCATT TGTTGGTGGG AATAAAATAT TCTTGCTAAT 841 CGAACCACAC GCAGCAAACG GGCCAACACA ACCAATTGCC CCAAAACCAT CGGCTCCTGT 901 TTCTTGCACG TATTCATCTA ACACAGTGTA AAATATCGTG ACGACGAATG TGACAACGAT 961 AATCAAACCA TCAGCAACCC TCCCAGGGGT TTGGTGAAGA ATTTCTTGGG CCGTAGGCAA 1021 ATTATCCTTA ATCCCTAAAT CCAGCATGAA TAACCAAGGA AAGAGCCAAA GCCCATTCCC 1081 GTCATAAAGA TGTGAGATCA CTTTTTTCCT GGGAAGAACT //
Primer: pAxCALNL_F1 Sequence file: RDB15262_A7H9c.seq
>D04498H2_A7H9_2_pAxCALNL_F1_G04_19_ABI24.ab1 1 TTCATGTATC TTATCATGTC TGGATCTACG TAATACGACT CACTATAGTT CTAGATTAGG 61 AATGAGCTAC TGCATCTTCT ACCTGCTCAG TAATGTTGCC TTCGGTTTTC CTGACGAGGC 121 GATGGCCGAT GTAATGGTCT CCTGGCATTT CAAGAATCTC TTTTGCCGCC TTGTAAGTAG 181 TCTTGAATTG GCATTTGTGA TTGCCGCCTC CTTCAAGTTT TAGGTACATC GTAACATCAC 241 CCTTCAGAAC TCCGTCGCTG GCAGTAATTT TCTCGGTTGA TGGCTCCCAA TCAACACTTT 301 GGTTTTGCAT GATAGGACCA TCGGCAGGAA AGTTAACCCC AGTAAATTTG GATTTGTGGT 361 AGAAGGTGTT TCCTCTAAGG CTTATATGCG CACTGACTGA AGCGGACCCA CCATCTTCGA 421 ACTCCAACGA CCTTTCCCAT GACAGGCCTT CAGGAAATGC TTGTTTGAAA TAGTCTGGTA 481 TCTCTTCTGG ATATTTAGTA AATACTCTGT GGCCGTAACA GAACACGTGT GACACTAAGT 541 CAAACGCGAA AGGCATTGGC CCGCCCTCGG CCATTGTGAC GCGTAGTGTC ATCTCTTGAT 601 GTCCCTCGTA AGGTCTGCCT GTGCCTTCAC CTTCAATTGT GAACTCATGC CCATTGACGG 661 AGCCGTCCAT GTAGTACCTC ATCTTCATCT CTGGTTTAAT CACACTCACC ATGGTCTCGA 721 GCTTCGAAGC CTGACTTGGC AGCAAAGAAT TGCGAGCACC GCATTCTCAT ACAGCTTGAC 781 TTTGCTGTAT CCCTGTCATG GCTCAGATCT CGATACGATG GTCGATCTTG TGGGCATCTG 841 GCACTGCAAC ACCTTTTTCG CTTTGTAGAG TTTGAGTCAC ATCGTGATGG CACTCTCCAG 901 CCACAGAGCC ATGGTAACAT CACCCATCGA AACCATCACG CACGGTACTC ATCTCCATGA 961 TGTCACTTCA GTTTCTTCGC TGACTGCACA TTGGGCAAGT TCTTGCACTA CGATTATAGT 1021 AGCGGTGCTC T //

Mermaid

Distribution information

References and tips

Sequence information

References