Lentivirus vector plasmid of Fucci2-G1 phase probe. Component of Fucci2 cell cycle probe.
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Other clones in our bank
External Database human CDT1 Discosoma sp. RFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link] |
Remarks | Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件: レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。 |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
備考 | 【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。提供条件の言語は英語のみ。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15273 | mCherry-hCdt1(30/120)/ pCSII-EF | DNA solution |
Materials & Methods section:
The mCherry-hCdt1(30/120)/ pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15273). |
Reference section:
Sakaue-Sawano, A., Miyawaki, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473. |
Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Lentiviral plasmid list (English text) |
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Featured content | Fucci-related materials (en) (English text) |
Featured content | Fucci-related materials (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15273_A7H3p1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro, insert5' Sequence file: RDB15273_A7H3a.seq |
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>D04498I4_2_A7H3_EF1a-C_F_G01_19_ABI24.ab1 1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT 61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTCGCC 121 ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC 181 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG 241 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG 301 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG 361 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG 421 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG 481 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC 541 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC 601 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC 661 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC 721 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC 781 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GGGATATCCA 841 TCACACTGGC GGCCGCTCGA GCCCAGCCCC GCCAGGCCCG CACTCCGCGC CCCGGCCTCC 901 GCTACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG CCCCCGGACG CGACCAGGCC 961 AGGCCACCGG GCCCGCAGAG ACTGCGGCTG TCGGTGGACG AGGTTTCCAG CCCCAGTACC 1021 CCCGGAGCCC CAGACATCCC AGCCCTGCCC TTCTCCGGGC CAGAAGATAA GAATCCACCC 1081 CCGGCAGCAG TCAGCCGCCC CCACTGACAT CCGCGCAGGA CAGACTCATC TATCTAGAGT 1141 TACATCGAAG GATCAAGGCT TTAATCGAAT AATTCAATGC // |
Primer: PRE_rev (Pr0250) Region: PRE, insert3' Sequence file: RDB15273_A7H3b.seq |
>D04498I4-2_A7H3_PRE_rev_G02_20_ABI24.ab1 1 ACGGCATTCA CATAGCGTAA AGGAGCAACA TAGTTAAGAA TACCAGTCAA TCTTTCACAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT 121 AGATGGTGTC CTGGTCCTGC GCGGATGTCA GGTGGGGCGG CTGACCTGCT GCCGGGGTGG 181 ATTTCTTTAT CTTCTGGCCC GGAGAAGGGC AGGCTGGGAT GTCTGGGGCC TCGGGGGTAC 241 TGGGGCTGGA AACCTCGTCC ACCGACAGCC GCAGTCTCCT GCGGGCCGGT GGCCTGGCCT 301 GGTCGCGTCC GGGGGCGGCG GGCGGGCGGG CGCGCTTGCG GCTGCCACTG GTAGCGGAGG 361 CCGGGGCGCG GAGTGCGGGC CTGGCGGGGC TGGGCTCGAG CGGCCGCCAG TGTGATGGAT 421 ATCCCTTGTA CAGCTCGTCC ATGCCGCCGG TGGAGTGGCG GCCCTCGGCG CGTTCGTACT 481 GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAACTTGATG TTGACGTTGT 541 AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCTTGTAGGT GGTCTTGACC TCAGCGTCGT 601 AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT GCTTGATCTC GCCCTTCAGG GCGCCGTCCT 661 CGGGGTACAT CCGCTCGGAG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC 721 CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA 781 GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC 841 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA 901 CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGACAGGATG TCCCAGGCGA AAGGGCAGGG 961 GCCACCCTTG GTCACCTTCA GCTTGGCGGG TCTGGGTGCC CTCGTAGGGG CGGGCCCTCG 1021 CTCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCACGGAGC CTCCATGTGC ACCTTTGAAG 1081 CGCATGGAAC TCCATGGATG ATGGGCCATG GTATCTTCCT CGCCCTTGCT CACCATGTGG 1141 CGATTCTCTC GGAACCGTAG CGATGTCACG GAACACATGC CTG // |
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1psi Sequence file: RDB15273_A7H3c.seq |
>D04498I4-2_A7H3_CMV-Forward_G03_21_ABI24.ab1 1 TGGTCACCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT 661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT 721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG 781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT 841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG 901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC 961 AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGCCAGAC ATTATTGTCT 1021 GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGGCTTATT GAGCGCAACA GCATCTGTTG 1081 CAACTCACAG TCTGGGGGCA ATCAAGCAGC TCAAGCCAAG AATCCTGGAC TGTGTGGAAA 1141 GGAAAATTAC CCTTAAAAAG // |
Primer: BGH_rev2 (Pr0606) Region: bGH p(A), 3'LTR, PRE Sequence file: RDB15273_A7H3d.seq |
>D04498I4-2_A7H3_BGH_rev2_G04_19_ABI24.ab1 1 CCTTTCAACG ATCAGGTAGC ACGGGGGAGG GGCAACAACA GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT 121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC 301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG 361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG 421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG 481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT 541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT 781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA 841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAGGA AAGGTCCGCT 901 GGATTGAGGG CCGAAAGGGA CGTAGCAGAA GGACGTCCCG CGCAGAATCC AGGTGGCAAC 961 ACAGGCGAGC AGCCATGGGA AAGGGACGTC AGCTTCCCCG ACACACCACG GAATTGTCAG 1021 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAGCAGC GCGATGAGTC CGCCGTGTCA 1081 TAGGAGGGGG AAGCGAAGTC CGGATGGAGC TGACTGGTGG TGGCCATGCC CACCCAGCGT 1141 GGGGGGATTG GGCGTTCAGC // |
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori, Zeo, SV40 p(A) Sequence file: RDB15273_A7H3e.seq |
>D04498I4-2_A7H3_SV40pro_F_G05_20_ABI24.ab1 1 CCCGTCCCGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 961 AATTTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 1021 AAATGAATCG GCCCAACGCC GCGGGGGAGA GGCGGTTGCG TATTGGGGCG CTCTTCCGCT 1081 TCCTCGCCTC ACTGACTCGC TGCGCTCGGT TCGGTCCGGC CTGCCGGCGG AGCGGTAATC 1141 AGCCTCCACC TTCCAAAAGA GGCCCGGTTT AT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473. |
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original | Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP] |
reference | Singer, D., Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. EMBO J. 38 (2). pii: e99845 (2019). PMID 30523147. |
reference | Liu, Y., Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence. J. Cell Sci. 132 (13). pii: jcs229385 (2019). PMID 31189537. |
reference | Tan, Z., Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Sci Rep. 8 (1): 2890 (2018). PMID 29440645. |
reference | Ohgushi, M., A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells. Stem Cell Reports 9 (1): 58-66 (2017). PMID 28579391. |
reference | Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130. |
reference | Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246. |
reference | Abe, T., Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development 140 (1): 237-246 (2013). PMID 23175634. |
reference | Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP] |
user_report | Dehghankelishadi, P., Bosutinib high density lipoprotein nanoformulation has potent tumour radiosensitisation effects. J. Nanobiotechnology 21 (1): 102 (2023) PMID 36945003. [link to RRC of NBRP] |
user_report | Badiee, P., Intratumoral Anti-PD-1 Nanoformulation Improves Its Biodistribution. ACS Appl. Mater. Interfaces 14 (14): 15881-15893 (2022). PMID 35357803. [link to RRC of NBRP] |
user_report | Dehghankelishadi, P., Formulation of simvastatin within high density lipoprotein enables potent tumour radiosensitisation. J. Control. Release 346: 98-109 (2022). PMID 35447296. [link to RRC of NBRP] |
2024.04.04
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