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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mAG-zGeminin(1/100) / pT2KXIGdeltain

zGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.

Catalog number RDB15276
Resource name mAG-zGeminin(1/100) / pT2KXIGdeltain
Clone info. zGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name zebrafish zGeminin cDNA Galaxeidae stony coral GFP cDNA
Reference of insert sequence AB505860 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
zebrafish zGeminin Galaxeidae stony coral GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions:
          The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件:
          Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15276 mAG-zGeminin(1/100) / pT2KXIGdeltain DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mAG-zGeminin(1/100) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15276).

          Reference section:

          Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Transposon and plasmid, Kawakami laboratory (English text)
          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15276_A7H9p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F2 ()
          Sequence file: RDB15276_A7H9a.seq check
          >D04498I7_A7H9_2_EF1a-C_F2_A06_03_ABI24.ab1
              1 GATTGGGGGT TAAGGCCCGG TTCGCTCTCT TCCTCACCGG GTCTGCGGCG AGTTCTAGCT
             61 GAAGCTTCCT GCAGGTCGAC CGATCCTGAG AACTTCAGGG TGAGTTTGGG GACCCTTGAT
            121 TGTTCTTTCT TTTTCGCTAT TGTAAAATTC ATGTTATATG GAGGGGGCAA AGTTTTCAGG
            181 GTGTTGTTTA GAATGGGAAG ATGTCCCTTG TATCACCATG GACCCTCATG ATAATTTTGT
            241 TTCTTTCACT TTCTACTCTG TTGACAACCA TTGTCTCCTC TTATTTTCTT TTCATTTTCT
            301 GTAACTTTTT CGTTAAACTT TAGCTTGCAT TTGTAACGAA TTTTTAAATT CACTTTTGTT
            361 TATTTGTCAG ATTGTAAGTA CTTTCTCTAA TCACTTTTTT TTCAAGGCAA TCAGGGTATA
            421 TTATATTGTA CTTCAGCACA GTTTTAGAGA ACAATTGTTA TAATTAAATG ATAAGGTAGA
            481 ATATTTCTGC ATATAAATTC TGGCTGGCGT GGAAATATTC TTATTGGTAG AAACAACTAC
            541 ACCCTGGTCA TCATCCTGCC TTTCTCTTTA TGGTTACAAT GATATACACT GTTTGAGATG
            601 AGGATAAAAT ACTCTGAGTC CAAACCGGGC CCCTCTGCTA ACCATGTTCA TGCCTTCTTC
            661 TCTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA
            721 GAATTCCTCG ACGGATCCAC TAGTAACGGC CGCCAGTGTG CTGGAATTCG CCACCATGGT
            781 GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG TGAACGGCCA
            841 CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC AGATCCTGGA
            901 CCTGAACGTG ACCGAGGGCG CCCCCCCTGC CCTTCGCCTA CGACATCCTG ACCACCGTGT
            961 TCCAGTACGG CAACAGGGCC TTCACCAAGT ACCCCGCCGA CATCCAGGGA CTACTTTCAA
           1021 GCAGACCCTT CCCCGAGGGC TACCAACTGG AGAGGAGCAT GAACCTACGA GGAACAGGCC
           1081 ATCTGCACGC CACCAGCACA TCAGCATGAG GGCGACCTGC CTTCCTCTAC GACAACCAGG
          1141 GTTCGA
          //
          Primer: pAxCALNL_F1 ()
          Sequence file: RDB15276_A7H9b.seq check
          >D04498I7_A7H9_2_pAxCALNL_F1_B06_06_ABI24.ab1
              1 CTCAATTAAA TCTTATCATG TCTGGATCAT CATCGATTAC TCATAGGCCT CTTGTGTGAC
             61 CCCCTCAGGC TGGTTTTCAT TTTCTGCATT TGTGGATTTG ACAGCCACCT CTGCTTTGAC
            121 TCTCTTTGAA CCCTTAACTT GCTCGGCGCT CCACATCTTC CTTTTGGCCA CTGCTTTACC
            181 ATTCTCAATT ATACGACCAA GGTTTTTGTT TACAGCAGAG GGCTGAAGCA CCTGAAGCGT
            241 CCTCCTCCCC ATTCCTGCTC CAGATGTTGG CGCTACCAGG AATTTCTTGA TGTTTTCAGA
            301 CGGGTTTTCT GCATTCTTTG GTCTTCTGAT GGAACTCATC TCGAGCGGCC GCCAGTGTGA
            361 TGGATATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG GCCACGGCGT TCTCGTACAG
            421 CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG ATCCTGTGGT CGATCTTGTG
            481 GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG GTCTTGAAGT CGCACCTGTA
            541 GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG CCCTTCAGCA CGCCGTCCTC
            601 CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC TTCTTCTGCA TCACGGGGCC
            661 GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG AAGAAGCAGT CGCCCCTCAT
            721 GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG TAGGTCATGC TCCTCTCCCA
            781 GTGGTAGCCC TCGGGGAAGG TCTGCTTGAA GTAGTCCTGG ATGTCGGCGG GGTACTTGGT
            841 GAAGGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG TCGTAGGCGA AGGGCAGGGG
            901 GGCGCCCTCG GTCACGTTCA GGTCCAGGAT CTGGGTGCCC TCGTAGGGTT TGCCCTTGCC
            961 CTCGCCCTCG ATCACGAAGT TGTGGCCGTT CACGGGTGCC CCTCATGCAC AGCTTGATCT
           1021 TCATCTTCGG GCTTGATCAC GCTTCACCAA TGGGTGGGCG AAATTCCAGC ACACTTGGGC
           1081 GGGCCCGTTA CCTAGTGATC CGGTCGGAAG GAAATTTTCT TTTTGACCAA AAATGTGAAT
          1141 GAGAGAACCC AGCACAC
          //
          Primer: EF1a-N_R2 ()
          Sequence file: RDB15276_A7H9c.seq check
          >D04498I7_A7H9_2_EF1a-N_R2_C06_09_ABI24.ab1
              1 TTACGGGCAA GAATTGCAAG TTTGATTTGC ATTGGAAAGG GTCGCTGGCT TTTGTGTTAC
             61 ACGCCCCTTA TTTGTGCTTG ATTAGATGAT CCCCCTGCTC GAGGGCCCAT CTGGCCTGTG
            121 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA
            181 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC
            241 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA
            301 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGCGTGTACT GGCATTAGAT TGTCTGTCTT
            361 ATAGTTTGAT AATTAAATAC AAACAGTTCT AAAGCAGGAT AAAACCTTGT ATGCATTTCA
            421 TTTAATGTTT TTTGAGATTA AAAGCTTAAA CAAGAATCTC TAGTTTTCTT TCTTGCTTTT
            481 ACTTTTACTT CCTTAATACT CAAGTACAAT TTTAATGGAG TACTTTTTTA CTTTTACTCA
            541 AGTAAGATTC TAGCCAGATA CTTTTACTTT TAATTGAGTA AAATTTTCCC TAAGTACTTG
            601 TACTTTCACT TGAGTAAAAT TTTTGAGTAC TTTTTACACC TCTGTCAAGA ACTCCTGGAC
            661 AAACCTCTGA CCTGTGTGGA ACAGAGTGGA TATGGGTGTC TGAACAGATA TTCACGTCTT
            721 TTGCAGATCA GAGGGCATTT CTGGTGTTGT GCAATGACCT GGGTCCAACA GGTAGGCGTA
            781 CTCGTATCCA AGAGCATTGT TGGACAGGAG GTAGTCTCCA TTCCTATAAA GAGGAAGGAA
            841 TGGCACCATG TAGTAGCCGT CATTGTGGCC AATTGGTGCA TTGGTACGTG GGTAGATGGA
            901 CCGGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAA
            961 CAAATATGCA AGATGAAAGT TAAGATAAGA TAAAAAAATT TAAACAAATA AATGCAATTT
           1021 ATGTTTTGTG TTTCTATTTC CCACCCAATA ATTAACCTCA CGCAGTAAAG CCCACCAGAA
           1081 TATTTTTTTG ATCCTTGCTC TGGCAGGCAG TCGCAGCTCG TAACGACTGG AATTCTCCAA
          1141 AAT
          //
          Primer: pAxEF_R2 ()
          Sequence file: RDB15276_A7H9d.seq check
          >D04498I7_A7H9_2_pAxEF_R2_D06_12_ABI24.ab1
              1 CGGGGAAAAA AGGCTTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
             61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
            121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
            181 ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG TGCTCTTGAT CCTGTGGCTG
            241 ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT ACTTCCTCCA TTTTCTTCCA
            301 CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG TGCTGTGCAT TGTGGTAATA
            361 GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG TCTGACCAAT TTCATATAAT
            421 GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA TTAAACTGGG CATCAGCGCA
            481 ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA GTGATCTCCA AAAAATAAGT
            541 ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA CTTTTTTTTC TAAAAAAATG
            601 TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT CAAGTAAAGT AAAAATCCCC
            661 AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC AAGTACTTTA CACCTCTGGT
            721 TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA GTTCTAGAGC GGCCGCCACC
            781 GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC
            841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG
            901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAA CTCACATTAA
            961 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC CTGTCGTGCC AGCTGCATTA
           1021 ATGAATCGGC CAACGCGCGG GGAGAGGCCG GTTTGCGTAT GGGCGCTCTT CGCTTTCCTC
          1081 GCTTCACTGA ACTTCGGCTG CCGCTCGGGA TCGATTCGGG CCTGGCCGGG CCGTAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl