Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Va (Brain type)

Expression vector of mouse brain-type myosin Va (Myo5a).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002). Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al., J. Biol. Chem. 277 (14): 12432-12436, 2002)。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15283_A7Gop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(FLAG,mMyosin Va(Brain type 5')) Sequence file: RDB15283_A7Goc.seq
>D04773A1_A7Go_1_EF1a-C_F_A07_01_ABI24.ab1     1 NNNNNNNNNN NNTTTTGANT TTGGNNCTTG GTTCNTTCTC NAGCCTCAGA CAGTGGTTCA    61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC   121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CTATGGCCGC GTCCGAGCTC   181 TACACCAAGT TTGCCAGGGT TTGGATCCCT GATCCTGAGG AAGTGTGGAA ATCGGCAGAG   241 TTGCTCAAGG ATTATAAGCC TGGAGATAAA GTGCTCCTGC TTCACCTCGA GGAAGGGAAG   301 GATTTGGAAT ACCGTCTAGA CCCAAAGACC GGTGAGCTCC CTCACTTACG GAACCCTGAC   361 ATACTTGTTG GAGAAAATGA CCTCACAGCC CTCAGCTACC TTCACGAGCC CGCTGTGCTA   421 CATAATCTCC GAGTTCGCTT CATCGATTCC AAACTTATTT ATACGTATTG TGGAATAGTT   481 CTGGTAGCTA TAAATCCCTA TGAGCAGCTG CCTATTTATG GAGAAGATAT TATTAATGCC   541 TACAGTGGCC AGAACATGGG TGACATGGAT CCTCACATCT TCGCAGTAGC TGAAGAGGCT   601 TACAAGCAAA TGGCAAGGGA TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCAGGC   661 GCAGGGAAGA CGGTCTCTGC TAAGTATGCC ATGCGGTACT TCGCAACTGT AAGTGGCTCT   721 GCCAGTGAGG CCAATGTTGA NGANNAGGTC TTGGCCTCCA ACCCCATCAT GGAGTCAATT   781 GGAAATGCCA AAACAACCAG GAATGATAAT AGCAGCCGGT TTGGAAAATA TATTGAAATT   841 GGTTTTGACA AGANGTACAG ANTCATCGGN GCCNATATGA GAANTTACCN TTTTAGANAA   901 ATCCAGANNG GNNNTCCAGG NAGANNNNGN ANANAAACTA NNNNANCTTC NATCANCNNN 961 NNNNNNN //
Primer: hGCSF_R (Pr0031) Region: hG-CSF polyA,insert 3'(mMyosin Va(Brain type 3')) Sequence file: RDB15283_A7God.seq
>D04773A1_A7Go_1_hGCSF_R_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNAGGG TCNNNGGNGG GGNNCCTCAC TCTAGAGCGG CCGCTATGGT    61 CGACGGTATC GATAAGCTTG ATATCGAATT CGATTCAGAC CCGTGCGATG AAGCCCAGGC   121 CCAGGCTGGC TGGGATCTGG ATGGTTTCTA GGGCTAGGGA GGATGGGTTA AAGGGAAAAG   181 TGACAGGAAA GATGTGTTTT GCATCCATGA GCAGCTGTGG AGAGTCTTTC CTGTCTCGTA   241 ACCGCATCTG TATAGTGCGG ATAAATGAAA CTGAGACCCT TTCTTCAAAC TCATTAACCG   301 GTGTGTACAG ATTCAACACT TTGACAATCT GGGCCGTGGT CAGGGCGTTG CACATGGAAC   361 AGATGGCTTC TGCGTCATCG TCAGTTTTCT TTTTCACTTG CAAAAGCTGA GCGGCCTGGA   421 TAAGAGGTTC CAGAGTCTCC TTGGCCCCAC TGTTCATTAG ATTCTTGTCA CGTAGCCATT   481 CTTCCAGTTG ACTGACATTG TACCTGATCT GCATGCCCTT GCTCCAGGAG CACATGTCCT   541 TGCGCAGGAG GAGGTTGTTT AGGGTGATGG CGCCCACAAT GTAGAACATC TGTTTGACTA   601 CCTGCTTGAT TAGCTCCGGG TCCATGCCAT GCTGACACAT GACTGAGTGG AAGGAGTTGA   661 GCTGCCGAAN GATGGAGTCC AGTGTGTAGG TGCCCTCATC TGCGATACTG GANGTTCGTT   721 TTCTGAGCCC TGTGGGCTTC ACCCCAGATA CGCCCTGAAT TGTTTCATGC TCTAGCATAC   781 CTGANACTAT CATTGGCTGA AGAATGNTCT CTAACACCCT CACAAGCTGC TGATAGATCT   841 GAATGGNCNA GTCACTCAGT ACTNGCNGAT ATTCAGCAAG GNCAAAATTG GTGANGNAGN   901 GCTCATTCTG GNGANACGNG TNNNGCTTCN TAAAGCCCNN CNNNNCNCTA TATNGNTTNN 961 NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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