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pEGFP-C1-human DENN

Expression vector of human DENN (MADD).

Catalog number RDB15288
Resource name pEGFP-C1-human DENN
Clone info. Expression vector of human DENN [MADD in NCBI], fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human MADD cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
human MADD

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Imai, A. et al., Arch. Biochem. Biophys., 536 (1): 31-37, 2013).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, A. et al., Arch. Biochem. Biophys., 536 (1): 31-37, 2013)。
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          RDB15288 pEGFP-C1-human DENN DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Imai, A., MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. Arch. Biochem. Biophys. 536 (1): 31-37 (2013). PMID 23702376.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15288_A7Gop1_2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Sequence file: RDB15288_A7Goa.seq check
          >D04773A6_A7Go_2_EGFP-C2_E07_13_ABI24.ab1
              1 GACCGAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
             61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGTGCAAA AGAAGAAGTT
            121 CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC AGGCACCCGA GCAGTGATAG
            181 CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG GAGGATCACA CTGAGTTTCC
            241 CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC TGCCTGAGCG TGCGGCAGCG
            301 GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC CTCACTGACA AGGACACTGG
            361 AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC TTCCAAAAGC GAATCTCTAA
            421 GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA GGAACCCATG CCACCTGTGC
            481 CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA TCCCTGCAGC CTCTCAGTGC
            541 TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA CGCCGGGCCA AGGCGGGGAG
            601 CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC AGCCACTACC CTTTCTTCTC
            661 CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG GACTGCTGTA GTGAGCGCCT
            721 TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG GACACCATGT GGCGGATCTT
            781 TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT CTGCATGACC TTCGAGAGAT
            841 TGAGGCCTGT ATCTATCGAT TGCTGCGCTC CCCAGTACCC GTCTCTGGGC AGAAGCGAGT
            901 AGACATCGAG GGTCCTACCC CAAGAGCTCC AGCCAGCTCT GACCTTTGCT CTTCCAGACC
            961 CATCTCGATT CACCCTAGTG GATTTCCCAC TGCACCTTCC CTTGACTTCT AGTGTGACGC
           1021 CTGTCTCCAG TGCTAACTTG CAATCCTGGT AGAGCACAGG TTGAGCTACA GTCCGAGAAC
          1081 TACATGCACT CCTCCATGGT CCT
          //
          Primer: pAxEF_R2
          Sequence file: RDB15288_A7Gob.seq check
          >D04773A6_A7Go_2_pAxEF_R2_F07_16_ABI24.ab1
              1 CAGTCAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
             61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
            121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
            181 ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC AGAATTCGAA
            241 GCTTGAGCTC GAGATCTTCC TCACTGCTAT GAACTGCAGC CACGTACGAG AAGAGACATA
            301 ATACGGAGTA GCAGATTTCG TGGGCCATTG GTGTCTTGTA CTTGTGGCTC ACCACTTCTT
            361 TAATTTCAGG AACTGATTGT GCATGAATTT GAGGTTGATC CCTTCCAGGG TCACCTGCAG
            421 CAGGCCACCC TCACCAGTCT TCAGGTCCTG CACAGGGAAC TCGCCACCCA ATTCAGGTCC
            481 GGGTTTGATG CTTTGCTGTC GGGCGGCAGC GCGCTCCATG CTGTCCTTCA CACAGTAGTA
            541 CAGCTCCCGA CACTTTTTAG TATAGAACTT GTTGAGCTGG GTCTCAGAGC CATTTCTACG
            601 CCACAAGCAC AACACCTTCG TCTTGGGACA GTAGGTCATG TTGATGAGCT TCTCGTACCA
            661 CCAGCGCTCA TACACTGTTC CGATGTTACT ACGCAACACC ACACAGTCAT CGCACACCTC
            721 CATGAAAAAG ATATCTCCGT TTGTATCTGT CCCTGCATGT ACCACAAATG TCTGCTTCTT
            781 CATGTGCCGG CTGCCACTGG ACCAGATAGA GAGATCGCGT CCATTCACGT TCGCCAGCTG
            841 ATCAAGCACC TCATTGATTT GCTGGCTGTA CACAAGCCCA ATGTGCGACT TTCCCATTAG
            901 CGCCTCACCT TCTTGCGGAT GTCATTCTTA TTTACCTTCA TCAGCAGCAT GTAGAGATGA
            961 GGTTGTGCAG AAGTGTGGCC AGCAAGCGAT CTTCATCATC TCCAGGCGCT TTCCGGTCAT
           1021 GTCTCAAGGA CAGGTACTGT CGATCATTTC TGGGGACCCT GATCATACCA ATCCATCTCT
          1081 CTCCAACATT CACAAGCCA
          //
          Primer: CMV-Forward
          Sequence file: RDB15288_A7Goc.seq check
          >D04773A6_A7Go_2_CMV-Forward_G07_19_ABI24.ab1
              1 CGTCCTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
            121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
            181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
            241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
            301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
            361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
            421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
            481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
            541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
            601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
            661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
            721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
            781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT
            841 CTATGGTGCA AAAGAAGAAG TTCTGTCCTC GGGTTACTTG ACTATCTAGT GATCGTAGGG
            901 CCCAGGGCAC CCGAGCAGTG ATAGCGTGGC CCAGACTCCT GAATTGCTAC GGCGATACCC
            961 CTTGTAGGAT CACACTGAGT TCCCCTGCCC CCAGATGTAG TGTCTCCTGG CAAGCCGAGG
           1021 GCTGCTGAGC GTGCGCAGCG ACGCAATGAT CCTTCGATGG ATACATCTTG CTTCACCGTT
          1081 CAATTGACAG A
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15288_A7God.seq check
          >D04773A6_A7Go_2_SV40pro_F_V2_A10_01_ABI24.ab1
              1 ACCAGGCTTT TTTTGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
            901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTTATGAAA GGTTGGGCTT
            961 CGGAATCGTT TTTCCGGACG CCGGCTGGAT GATCCTCCAG CGGCGGGGGA TCCTCATGCT
           1021 GAGTTCTTCG CCCACCTAGG GGGGAGGCCT ACCTGGAAAC ACGGAAAGGG AGACAATACC
           1081 GGAAGACCCG CGCCTTATTG ACCGCAAATA AAGACCGATA ACGGCAACGG TTTGGTTCGG
          1141 TTTGTTTCTC ATAAACGTCG GGGGGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Imai, A., MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. Arch. Biochem. Biophys. 536 (1): 31-37 (2013). PMID 23702376.

          2023.04.06

          GNP_filter3_RDBDEP_html_230405.pl