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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rabex-5 (#RDB15291)

Expression vector of mouse Rabex-5 (Rabgef1).

Clone info. Expression vector of mouse Rabex-5 [Rabgef1 in NCBI], fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rabgef1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15291 pEGFP-C1-mouse Rabex-5 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rabex-5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15291).

Reference section:

Mori, Y., Matsu,i ., Fukuda, M., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15291_A7Gop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB15291_A7Goa.seq check
>D04773A9_A7Go_2_EGFP-C2_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNCGCGNNCN CNTGGTCCTG CTGGNNTTCG TGANCGCCGC CGGGNTCACT
   61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTA TGAGCCTGAA GTCCGAACGC
  121 AGGGGAATTC ATGTGGATCA ATCTGAGCTC CTGTGCAAGA AAGGATGCGG TTACTACGGC
  181 AACCCTGCCT GGCAGGGTTT CTGCTCCAAG TGCTGGAGGG AGGAGTACCA CAAGGCCCGG
  241 CAGAGGCAGA TCCAAGAGGA CTGGGAACTG GCAGAACGAC TTCAGCGGGA GGAGGAAGAG
  301 GCCTTCGCGA GCAGCCAGAG CAGCCAAGGA GCCCAGTCCC TCACCTTCTC CAAGTTCGAG
  361 GAGAAGAAGA CCAATGAGAA AACCCGAAAA GTCACCACAG TGAAGAAGTT CTTCAGCGCC
  421 TCTTCCAGAG CTGGATCCAA GAAGGAAATT CAGGAAGCCA AAGCTCCCAG TCCCTCCATA
  481 AACCGGCAAA CCAGCATTGA GACGGACCGA GTGACTAAGG AGTTCATAGA CTTTCTCAAG
  541 ACCTTCCACA AGACAGGCCA AGAAGTCTAT AAACAGACGA AGATGTTTTT GGAAGCAATG
  601 CCTTATAAAA GGGATTTAAG CATCGAGGAA CAGTCAGAAT GTACTCAGGA CTTTTACCAA
  661 AATGTGGCTG ANAGAATGCA GACCCGTGGG AAAGTGCCTC CAGAGAAAGT GGAGAAGATA
  721 ATGGATCAGA TCGAAAAGCA CATCATGACG CGTCTCTATA AATTTGTGTT CTGCCCAGAG
  781 ACTACTGATG NNGAGAAGAA AGATCTCGCC ATTCAAAAAA GANTCNNGGC CCTGCACTNN
  841 NNAACGCCTC ANATGCTCTG NGTCCNNGNC NNNGANGAAA TCCCNGAAGN GNNCNANNTG
901 GNGGNGAAAN CGATCNCAGA NNTCNNNNAN ATGGNNTNNA NNCNNNNNNN NNN
//
Primer: pAxEF R2 (Pr0059)
Region: SV40 pA,insert 3'
Sequence file: RDB15291_A7Gob.seq check
>D04773A9_A7Go_2_pAxEF_R2_F09_18_ABI24.ab1
    1 NNNNNNNNNN NGNNTNNNTT GTGANNTTGT GNNGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC
  241 GCGAATTCAC TAGTGATTTC ACCCTGCGTA CACCTGAGGC TGCAGAGGGG GAGGGAGCTT
  301 GTCGTTCTCC ACATTCTCAG AGTCGATGGC TGCTAAGGGT TGGTTCGGGG GCTTAATCTC
  361 CAGTGGGTAT TTCTCAACAA TGTCTTGAAC TTCCTTGGCA ATCCCGTCTG TCCAGTCTAT
  421 TAAGTCTTTT TCAAGTTTCT TGGCTTCGTT CATGATCCTT TCTTGCCGTT CATTCAACTG
  481 AGACAGGAGG TCCAAGTTCT TATACATTTG CTTCACACCT AAGCAGGCCT CCGGGGGCCA
  541 ACTCTCAGAC TCCTGCTTCC TGGGGGATGT CTGGCCAGAC ATGTACCGGT CAAAATCCTC
  601 CTGACTTAAA TTCAAAGACT GGGCGTCTAA TTTCTCAATG AAAGCCACAG CACAGCACAG
  661 GTTGGTGAAG TAGTAGCCAT CCTCGCCCGT CATGAGCCGG CTGGGGTTGC AGAAGCGAGT
  721 GANGTACTGG ATGTTGGACT GCANGCGANG GGGGTTGCCC TTCANGACGA TGTAGATCAG
  781 GGTGGGCAGG AAGTCATCGG CAGANGCTGG CTCATTCTTG GTGANCTTGA TGGCATTGAA
  841 NANGTGCTTG CTGCACCTGG TGATGCANGC CAGCTTGNCC CNANGNACAC GCTTTGNNNC
  901 CATCNNNNNG ANGNCNGNGN NCGCTTTCNN NNNCANNNCN NANNNNTCNN NNNNTTNCNC
961 NNNGNNNNGN NNNNNNNNN
//
Primer: Rabex-5-M_F (Pr0931)
Region: insert mid
Sequence file: RDB15291_A7Goc.seq check
>15291_15291_B0L7F_Rabex-5-M_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNTCN CNGACATCAT TGAGATGGAC TCAAAGCGTG
   61 TGCCTCGGGA CAAGCTGGCC TGCATCACCA GGTGCAGCAA GCACATCTTC AATGCCATCA
  121 AGATCACCAA GAATGAGCCA GCCTCTGCCG ATGACTTCCT GCCCACCCTG ATCTACATCG
  181 TCCTGAAGGG CAACCCCCCT CGCCTGCAGT CCAACATCCA GTACATCACT CGCTTCTGCA
  241 ACCCCAGCCG GCTCATGACG GGCGAGGATG GCTACTACTT CACCAACCTG TGCTGTGCTG
  301 TGGCTTTCAT TGAGAAATTA GACGCCCAGT CTTTGAATTT AAGTCAGGAG GATTTTGACC
  361 GGTACATGTC TGGCCAGACA TCCCCCAGGA AGCAGGAGTC TGAGAGTTGG CCCCCGGAGG
  421 CCTGCTTAGG TGTGAAGCAA ATGTATAAGA ACTTGGACCT CCTGTCTCAG TTGAATGAAC
  481 GGCAAGAAAG GATCATGAAC GAAGCCAAGA AACTTGAAAA AGACTTAATA GACTGGACAG
  541 ACGGGATTGC CAAGGAAGTT CAAGACATTG TTGAGAAATA CCCACTGGAG ATTAAGCCCC
  601 CGAACCAACC CTTANCAGCC NTCGACTCTG ANAANGNGNA GAANGACNAG CTCCCTCCCC
  661 CTCNGCNGCC TCNNGTGTAN GCNNNNGAAA TCACTANNGN ATTCGCNGNN GNCNNCNNNC
721 NNNNNNANNN CGGGNCNGGG NNNCNNCNNN NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB15291_A7God.seq check
>D04773A9_A7Go_2_CMV-Forward_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNTNGNNG CNNNGGGCGG TANGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC
  721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAANCGCG ATCACATGGT CCTGCTGGAN
  781 TTCGTGANCG CCGCCGGGNT CACTCTCGGC NTGGNCGANC TGTACAANNC CGGANTCAGA
  841 TCTATGANCC NGAAGTCCNA ACGCANGGGN ATTCATGNNN NTCNNTCTGA NCTCCNGNGC
901 NNNAAANGAN GCNNTTANNA NNNNNNNCNN NNCNNNNNNG NNTNNNGNNN NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15291_A7Goe.seq check
>D04773A9_A7Go_2_SV40pro_F_V2_H09_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN GCTTTTGNNN NNNCGNTCAA GAGNNAGGNN GAGGNNCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
  721 NGACATAGCG TTGGCTACCC GTGATATTGC TGAAGANCTT GGCGGCGAAT GGGCTGANCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAN TTCTTCTGAN CGGGANTCNN GGGGTNCGAA ATGANCNNNC AAGCGACGCC
  901 NNANCNNGNC NTCACNANNT TTCNATTNCN CCNNNNNCNT TCNNNNNAAN GNNNNGGNNN
961 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP]