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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rabex-5-D313A (#RDB15292)

Expression vector of mouse Rabex-5 (Rabgef1).

Clone info. Expression vector of mouse Rabex-5 [Rabgef1 in NCBI] GEF activity-deficient mutant (D313A), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rabgef1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15292 pEGFP-C1-mouse Rabex-5-D313A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rabex-5-D313A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15292).

Reference section:

Mori, Y., Matsu,i ., Fukuda, M., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15292_A7Gop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB15292_A7Goa.seq check
>D04773B1_A7Go_2_EGFP-C2_D06_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNCNNNN NNNNNTGGTC CTGCTGGNNT TCGTGNNCGC CGCCGGGNNC
   61 NCTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGAGCCT GAAGTCCGAA
  121 CGCAGGGGAA TTCATGTGGA TCAATCTGAG CTCCTGTGCA AGAAAGGATG CGGTTACTAC
  181 GGCAACCCTG CCTGGCAGGG TTTCTGCTCC AAGTGCTGGA GGGAGGAGTA CCACAAGGCC
  241 CGGCAGAGGC AGATCCAAGA GGACTGGGAA CTGGCAGAAC GACTTCAGCG GGAGGAGGAA
  301 GAGGCCTTCG CGAGCAGCCA GAGCAGCCAA GGAGCCCAGT CCCTCACCTT CTCCAAGTTC
  361 GAGGAGAAGA AGACCAATGA GAAAACCCGA AAAGTCACCA CAGTGAAGAA GTTCTTCAGC
  421 GCCTCTTCCA GAGCTGGATC CAAGAAGGAA ATTCAGGAAG CCAAAGCTCC CAGTCCCTCC
  481 ATAAACCGGC AAACCAGCAT TGAGACGGAC CGAGTGACTA AGGAGTTCAT AGACTTTCTC
  541 AAGACCTTCC ACAAGACAGG CCAAGAAGTC TATAAACAGA CGAAGATGTT TTTGGAAGCA
  601 ATGCCTTATA AAAGGGATTT AAGCATCGAG GAACAGTCAG AATGTACTCN GGANTTTTAC
  661 CNAAATGTGG CTGNNNAATG CANANCCGNN GNAAAGTGNC TCCAGANAAA GTNNNNAAGA
  721 NAATGGNTCA NATCGAAAAG CACNTCNNGN NNNNTCTCTA TAAATTTGTG NNCTGNCCAN
781 ANNCTACNNN NNANGANAAN AAANNANNNN NNNNNN
//
Primer: pAxEF R2 (Pr0059)
Region: SV40 pA,insert 3'
Sequence file: RDB15292_A7Gob.seq check
>D04773B1_A7Go_2_pAxEF_R2_E06_05_ABI08.ab1
    1 NNNNNNNNNN GNNTNNNTTG TGAANTTGTG NNGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACCTG CAGGCGGCCG
  241 CGAATTCACT AGTGATTTCA CCCTGCGTAC ACCTGAGGCT GCAGAGGGGG AGGGAGCTTG
  301 TCGTTCTCCA CATTCTCAGA GTCGATGGCT GCTAAGGGTT GGTTCGGGGG CTTAATCTCC
  361 AGTGGGTATT TCTCAACAAT GTCTTGAACT TCCTTGGCAA TCCCGTCTGT CCAGTCTATT
  421 AAGTCTTTTT CAAGTTTCTT GGCTTCGTTC ATGATCCTTT CTTGCCGTTC ATTCAACTGA
  481 GACAGGAGGT CCAAGTTCTT ATACATTTGC TTCACACCTA AGCAGGCCTC CGGGGGCCAA
  541 CTCTCAGACT CCTGCTTCCT GGGGGATGTC TGGCCAGACA TGTACCGGTC AAAATCCTCC
  601 TGACTTAAAT TCAAAGACTG GGCGTCTAAT TTCTCAATGA AAGCCACAGC ACAGCACAGG
  661 TTGGTGAAGT AGTAGCCATC CTCGCCCGTC ATGAGCCGGC TGGGGTTGCA GAANCGAGTG
  721 NTGTACTGGA TGTTGGACTG CAGGCGANGG GGGTTGCCCT TCANGACGAT GTANATCANG
  781 GTGGGCAGGA ANTCCGCGGC ANANGCTGGC TCATTCTTGG TGANCTTGAT GGCATTGAAN
  841 ANGTGCTTGC TGCACCTGNN GATGCNNGNC AGCTNNNCCC GANGCACACG CTTTGANTCC
  901 ATCTNNNNGA TGTCNGNGAT CGCTTNCNNC NNNNTNNNNN ANNNNNCNNN NNTNNNNNNN
961 NNN
//
Primer: Rabex-5-M_F (Pr0931)
Region: insert mid (D313A)
Sequence file: RDB15292_A7Goc.seq check
>15292_15292_B0L9F_Rabex-5-M_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNAAGCGNT CNCAGACNTC ATTGAGATGG NACTCAAAGC
   61 GTGTGCCTCG GGACAAGCTG GCCTGCATCA CCAGGTGCAG CAAGCACATC TTCAATGCCA
  121 TCAAGATCAC CAAGAATGAG CCAGCCTCTG CCGCGGACTT CCTGCCCACC CTGATCTACA
  181 TCGTCCTGAA GGGCAACCCC CCTCGCCTGC AGTCCAACAT CCAGTACATC ACTCGCTTCT
  241 GCAACCCCAG CCGGCTCATG ACGGGCGAGG ATGGCTACTA CTTCACCAAC CTGTGCTGTG
  301 CTGTGGCTTT CATTGAGAAA TTAGACGCCC AGTCTTTGAA TTTAAGTCAG GAGGATTTTG
  361 ACCGGTACAT GTCTGGCCAG ACATCCCCCA GGAAGCAGGA GTCTGAGAGT TGGCCCCCGG
  421 AGGCCTGCTT AGGTGTGAAG CAAATGTATA AGAACTTGGA CCTCCTGTCT CAGTTGAATG
  481 AACGGCAAGA AAGGATCATG AACGAAGCCA AGAAACTTGA AAAAGACTTA ATAGACTGGA
  541 CAGACGGGAT TGCCAAGGAA GTTCAAGACA TTGTTGAGAA ATACCCACTG GAGATTAAGC
  601 CCCCGAACCA ACCCTTANCA GCCATCGACT CTGANAANGT GNAGAANGAC AAGCTCCCTC
  661 CCCCTCNGCA GCCTCNNNGN ANNCNNNNGA ANTCNCTANN GNATTCNCGG NNGNCNNNNG
721 NCNANNNNNN NNCGGNNCNN GNNNNNNNCN NNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB15292_A7God.seq check
>D04773B1_A7Go_2_CMV-Forward_F06_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA ANNGGGCGGT AGGCGTGTAC GGTGGGNNGT
   61 CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC
  121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
  421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCNANCA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACN ACAGCCACAA CGTCTATATC ATGGNCGACA AGCANAANAN
  601 CGGCATCNAG GTGAACTTCN AGANCCGCCN CAACATCNAG GANGGCAGCG TGCAGCTCGN
  661 CNANNNCTAN NNNCAGAACA CCCCCNTCGG CGANNGCCCC NNGCTGCTGC CCGACNACCA
  721 CTNCCTGANN NCCCNNTNCN CCNNGANCNA AGACCCNNNC NANANNCNNN ATNNCNTNNN
781 NNTGNTNNNN NNNNNGNNNN NNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15292_A7Goe.seq check
>D04773B1_A7Go_2_SV40pro_F_V2_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CTTTNGCNNN NNCGATCAAG AGACAGGNNG AGGNNCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ANCTCGTCGT GACCCATGGC GATGCCTGCT TGCCNAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CNTCGACTGT GGCCGGCTGN GNGTGGCGGA NCGCTATCNN
  721 GANNTANCGT TGGNTANCCG NGANATTGCT GNANANCTTN NNGGCGAANG GGCTGANCGC
  781 TNCCNCGNGN TTTANNGNAT CGNNNCTCCC NATTNNNANC GNATCNNNTT CNANCNNNNN
  841 CNNNNNNANN NNNNCNNANC NGNNNTCNNG GGNNCNAANN GANNNANCNN NCNNNNNCCN
901 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP]