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pEGFP-C1-mouse Ccz1 (#RDB15295)

Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yasuda, S., J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1), fused with N-terminal EGFP, CMV promoter. Recruited to the late-endosomal state of macropinosomes (Fig.5 of Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2011)..
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name mouse Ccz1 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016)。

Catalog # Resource name Shipping form Fee
RDB15295 pEGFP-C1-mouse Ccz1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Ccz1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15295).

Reference section:

Yasuda, S., Morishita, S., Fujita, A., Nanao, T., Wada, N., Waguri, S., Schiavo, G., Fukuda, M., Nakamura, T., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [PubMed] [Article] [RRC of NBRP]