Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-JF1_886-1349

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1349, sense orientation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15329_A7Hmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB15329_A7Hma.seq
>D04779A3_A7Hm_1_pGL4-4174F_C04_07_ABI24.ab1     1 ACTGGGTCAG ACATTTCTCT GGCTAACTGG CCGCCCTGAA GGCAATTTCT AAGTTGGATA    61 GTTACAGACT GTTTATAATT GGACCTATTA TTACCTTGCA TAAGTTAGCT ATGTTGAGGT   121 TGCAAAAGGA CGGGAGGTTA CTCAGGATGG AAACTGTTTG GAATAGAACA AGGACATGCC   181 CAAACCGTTT CAATTATCTG TGTTTGTTTG TTTTGTGTCA TGATCCTGAA AGAAATTTTG   241 TGTAGGAGTT CAATCTTAAA ATGAGCGTTG AGTTGCAGTC TGGCAGCACA TTTTAAAATT   301 AAGAGGGGAA CAGGGAGGAG GGGGAGAAGA AGGAGGAAGA AAAAGAAGAA GAGGAGGAGG   361 AAGAGGAAGA AGAGGAGGAG GAAGAGGAAG AAGAGGAGGA GGAGGAGGAG GAGGAGGAGG   421 AGGAGGAGGA GGAGGAGATC TTGAACTCAG CCAATCAGGT TCAGAGGCCC ATTACTTAGG   481 TAGCTTCCCG TCCTTGGGCC TCGGCGGCCA AGCTTAGACA CTAGAGGGTA TATAATGGAA   541 GCTCGACTTC CAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA   601 AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG   661 CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA   721 CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA   781 GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC   841 TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT   901 AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC   961 GTATTCGTGA GCAAGAAAGG GGCTGCAAAA GATCCCTCAA CGTGCAAAAA GAAAGCTACC  1021 CGATCATACA AAGATCATCA TTCATGGGAA TAGCAAGACC GGACTTACTA GGCTTCGAAA 1081 AGCAATGTAC CAACCATTCG GTTGAACCTT TTC //
Primer: pGL4-136R Region: insert 3' Sequence file: RDB15329_A7Hmb.seq
>D04779A3_A7Hm_1_pGL4-136R_C05_08_ABI24.ab1     1 GTCTTTAGGC TTTTGGCATC TTCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTG    61 GAAGTCGAGC TTCCATTATA TACCCTCTAG TGTCTAAGCT TGGCCGCCGA GGCCCAAGGA   121 CGGGAAGCTA CCTAAGTAAT GGGCCTCTGA ACCTGATTGG CTGAGTTCAA GATCTCCTCC   181 TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCTCCTCTT CTTCCTCTTC CTCCTCCTCT   241 TCTTCCTCTT CCTCCTCCTC TTCTTCTTTT TCTTCCTCCT TCTTCTCCCC CTCCTCCCTG   301 TTCCCCTCTT AATTTTAAAA TGTGCTGCCA GACTGCAACT CAACGCTCAT TTTAAGATTG   361 AACTCCTACA CAAAATTTCT TTCAGGATCA TGACACAAAA CAAACAAACA CAGATAATTG   421 AAACGGTTTG GGCATGTCCT TGTTCTATTC CAAACAGTTT CCATCCTGAG TAACCTCCCG   481 TCCTTTTGCA ACCTCAACAT AGCTAACTTA TGCAAGGTAA TAATAGGTCC AATTATAAAC   541 AGTCTGTAAC TATCCAACTT AGAAATTGCC TTCAGGGCGG CCAGTTAGGC CAGAGAAATG   601 TTCTGGCACC TGCACTTGCA CTGGGGACAG CCTATTTTGC TAGTTTGTTT TGTTTCGTTT   661 TGTTTTGATG GAGAGCGTAT GTTAGTACTA TCGATTCACA CAAAAAACCA ACACACAGAT   721 GTAATGAAAA TAAAGATATT TTATTGCGGC CTGTCCAATA CCTCCCGTAC CTTAATATTA   781 CTTACTTATC CTTGAGAGAC GTACTAGTAA CCCTGATAAA TGCTTCAATA ATATTGAAAA   841 AGGACGAGTA TGAGCATCCA ACATTTTCGT GTCGCACTCA TTCCCTTCTT TGCGGCATTT   901 TGCTTGCCTG TTTTTGCACA CCCCGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAA   961 CTGGGTGCAA GAGTGGGGCT ATATCGAACT GGGATCTCAA TAGCGGGCAA GATCCTTTGA  1021 GTCTTTCCGC CCCGAAGACG ATTTCCCGAT GAATGAAGCA CTTTCAAGTA CTGCCTATGT 1081 GACGCGATGT GGTCCCGTAA TAGGACGCCC GGGCTAAGAA GC //
Primer: pAxCALNL_F1 Region: Luciferase 3' Sequence file: RDB15329_A7Hmc.seq
>D04779A3_A7Hm_1_pAxCALNLF1_F11_17_ABI24.ab1     1 ATAAGTATCT TATCATGTCT GCTCGAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC    61 GGCGATCTTG CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA   121 CTTGCCGGTC AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT   181 GGCGGTTGTA ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC   241 GTGTTCCAGC ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC   301 GACCCCGGCG TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC   361 TACCTGGTAG CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC   421 GTCCTCGTCC CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC   481 GTTTGTAGCC TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG   541 CTCGCCGCGC TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC   601 GAAGAAGGGC ACCACCTTGC CTACTGCGCC AGGCTTGTCG TCCCCTTCGG GGGTGATCAG   661 AATGGCGCTG GTTGTTTCTG TCAGGCCGTA GCCCTGGCGG ATGCCTGGTA GGTGGAAGCG   721 TTTGGCCACG GCCTCACCTA CCTCCTTGCT GAGCGGCGCC CCGCCGCTGG CGATCTCGTG   781 CAAGTTGCTT AGGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT   841 GGGCACCAGC AGGGCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAAGA ATAGCTCCTC   901 CTCGAAGCGG TACATGAGCA CGACCCGAAA GCCGCAGATC AAGTAGCCCA GCGTGGTGAA   961 CATGCCGAAA GCCCGTGGTG AAATGGCACC ACGCTGAGGA TAGCGTGTCG GGGAATGATC  1021 TGGGTTTGCC GAGATGGGGG TCGCGGCATG ACTGGATTCG ACACAGCGTT GCGCTGCGGT 1081 AAGGCTACGT CTCTTGTCAA GTCAGTACTG CCACTACGTT CATGATCAGG GAGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl