Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-JF1_1128-1312anti

Luciferase reporter of the mouse Ccnb1ip1, nt.1128 to nt.1312, antisense orientation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15332_A7Hmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB15332_A7Hma.seq
>D04779A6_A7Hm_2_pGL4-4174F_F04_16_ABI24.ab1     1 GCTGGTCAGA CATTTCTCTG GCTACTGGCC CCTGATTGGC TGAGTTCAAG ATCTCCTCCT    61 CCTCCTCCTC CTCCTCCTCC TCCTCCTCCT CCTCCTCTTC TTCCTCTTCC TCCTCCTCTT   121 CTTCCTCTTC CTCCTCCTCT TCTTCTTTTT CTTCCTCCTT CTTCTCCCCC TCCTCCCTGT   181 TCCCCTCTTA ATTTTAAAAT GTGCTGCCAG ACTGCGGCCT CGGCGGCCAA GCTTAGACAC   241 TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA TCCGGTACTG TTGGTAAAGC   301 CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA   361 CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC   421 CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT   481 GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT   541 GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG   601 TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG   661 CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA TCCTCAACGT   721 GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA   781 GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT TCAACGAGTA   841 CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG   901 TGGCAGTACC GGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCCGC TTGTGTCCGA   961 TTCAGTCATG CCCGCGACCC CCATCTTCGG GCAACCAGAT CATCCCCCGA CACCGGCTAT  1021 CCTCAGCGTG GTGCCCAATT TTCACCCACG CCTCGGCATT GATTCAACCC ACGCTGGACT 1081 AACTGGATCC TGCGGGCCTT TCGGGATCGG TGGCCCTCCA AT //
Primer: pGL4-136R Region: insert 3' Sequence file: RDB15332_A7Hmb.seq
>D04779A6_A7Hm_2_pGL4-136R_F05_17_ABI24.ab1     1 GACTTTGCAT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTGG    61 AAGTCGAGCT TCCATTATAT ACCCTCTAGT GTCTAAGCTT GGCCGCCGAG GCCGCAGTCT   121 GGCAGCACAT TTTAAAATTA AGAGGGGAAC AGGGAGGAGG GGGAGAAGAA GGAGGAAGAA   181 AAAGAAGAAG AGGAGGAGGA AGAGGAAGAA GAGGAGGAGG AAGAGGAAGA AGAGGAGGAG   241 GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGATCT TGAACTCAGC CAATCAGGGG   301 CCAGTTAGGC CAGAGAAATG TTCTGGCACC TGCACTTGCA CTGGGGACAG CCTATTTTGC   361 TAGTTTGTTT TGTTTCGTTT TGTTTTGATG GAGAGCGTAT GTTAGTACTA TCGATTCACA   421 CAAAAAACCA ACACACAGAT GTAATGAAAA TAAAGATATT TTATTGCGGC CTGTCCAATA   481 CCTCCCGTAC CTTAATATTA CTTACTTATC CTTGAGAGAC GTACTAGTAA CCCTGATAAA   541 TGCTTCAATA ATATTGAAAA AGGACGAGTA TGAGCATCCA ACATTTTCGT GTCGCACTCA   601 TTCCCTTCTT TGCGGCATTT TGCTTGCCTG TTTTTGCACA CCCCGAAACG CTGGTGAAAG   661 TAAAAGATGC TGAAGATCAA CTGGGTGCAA GAGTGGGCTA TATCGAACTG GATCTCAATA   721 GCGGCAAGAT CCTTGAGTCT TTCCGCCCCG AAGAACGATT CCCGATGATG AGCACTTTCA   781 AAGTACTGCT ATGTGGCGCG GTGTTGTCCC GTATAGACGC CGGGCAAGAG CAGCTTGGTC   841 GCCGTATACA CTACTCACAA AACGACTTGG TTGAGTACTC GCCGGTCACG GAAAAGCATC   901 TTACGGATGG CATGACGGTA AGAGAATTGT GTAGTGCTGC CATTACCATG AGCGACAACA   961 CCGCGGCCAA CTTACTTCTG ACAACGATCG GAGGCCCTAA GGAGCTGACT GCATTTCTTT  1021 CATAATTATG GGTTGATCAT GTGACCCGGG CTTGACCGCT GGCAAACCCA GAGGTGACCG 1081 AAGTCATTAC CGGAAACCGA ACGAGCCGGT GAATATACCC AACGCGA //
Primer: pAxCALNL_F1 Region: Luciferase 3' Sequence file: RDB15332_A7Hmc.seq
>D04779A6_A7Hm_2_pAxCALNLF1_F12_18_ABI24.ab1     1 AATAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA    61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC   121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT   181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT   241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC   301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG   361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG   421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG   481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA   541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC   601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT   661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA   721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC   781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG   841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC   901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT   961 GAACATGCCG AAGCCCGTGG TGAAATGGGC ACCACGCTGA GGATAGCGGT GTCGGGGATG  1021 ATCTGGTTTG CGAGATGGGG GTCGCGGGGC ATGACTGAAT CCGGACACAG CGATGCGCTG  1081 CGTAAGGCTA CGCCTTGTCA ATCCAGTACT GCCATACTGT TACATGAATC CAAGGGATCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl